FastQCFastQC Report
Thu 26 May 2016
SRR1548140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548140_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2305609
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT589792.5580660033856564No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT27320.11849363877396385No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT27190.1179297964225504No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23090.10014707610874177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG351.2126475E-745.0000041
CGAATCG207.034767E-445.023
CCGACGT207.034767E-445.043
AATCGTC302.1664564E-644.99999635
CGTTTTT239000.044.1433071
CGACGGT3950.041.5822828
GACCGAT5200.041.538469
TATAGCG2400.041.2499961
CACGACG4050.040.026
TCACGAC4100.039.51219625
CTCACGA4300.039.24418324
AGGGCGA13650.039.2307666
ATTAGCG1500.039.01
CGTTAGG3600.038.7500042
GTTTTTT279200.038.609422
TAGGGAC19950.038.4586455
GTTAGCG1300.038.076921
TACGGGA6150.038.048784
GTAGGGC7500.037.84
ATAGCGG4050.037.7777752
Produced by FastQC (version 0.11.5)