##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548140_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2305609
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1059667966251	31.0	31.0	33.0	30.0	34.0
2	31.47164588618452	31.0	31.0	34.0	30.0	34.0
3	31.377259110282793	31.0	31.0	34.0	30.0	34.0
4	35.29709677573257	37.0	35.0	37.0	33.0	37.0
5	35.19519007776253	37.0	35.0	37.0	32.0	37.0
6	35.27027913232469	37.0	35.0	37.0	32.0	37.0
7	35.73966661302936	37.0	35.0	37.0	35.0	37.0
8	35.74890495309482	37.0	35.0	37.0	35.0	37.0
9	37.48352647825369	39.0	37.0	39.0	35.0	39.0
10	36.79091337689955	39.0	37.0	39.0	32.0	39.0
11	36.472954434164684	39.0	35.0	39.0	32.0	39.0
12	35.92046960260825	37.0	35.0	39.0	31.0	39.0
13	35.67282830696792	37.0	35.0	39.0	30.0	39.0
14	36.63442804048735	39.0	35.0	41.0	31.0	41.0
15	36.87635544448343	39.0	35.0	41.0	31.0	41.0
16	36.979833961439255	39.0	35.0	41.0	31.0	41.0
17	36.838391071512994	38.0	35.0	41.0	31.0	41.0
18	36.66824340120116	38.0	35.0	40.0	31.0	41.0
19	36.49087681389169	38.0	35.0	40.0	31.0	41.0
20	36.24260661716709	37.0	35.0	40.0	31.0	41.0
21	36.10298667293544	37.0	34.0	40.0	30.0	41.0
22	36.041760766894996	37.0	34.0	40.0	30.0	41.0
23	35.97539825703318	37.0	34.0	40.0	30.0	41.0
24	35.78569263045035	37.0	34.0	40.0	30.0	41.0
25	35.62649477860296	37.0	34.0	40.0	30.0	41.0
26	35.534079282306756	36.0	34.0	40.0	30.0	41.0
27	35.44996267797359	36.0	34.0	40.0	29.0	41.0
28	35.41865728317334	36.0	34.0	40.0	29.0	41.0
29	35.37912239239177	36.0	34.0	40.0	29.0	41.0
30	35.229183699404366	36.0	34.0	40.0	28.0	41.0
31	34.99926917356759	36.0	34.0	40.0	27.0	41.0
32	34.68287554394522	36.0	33.0	40.0	25.0	41.0
33	34.368580275319886	36.0	33.0	40.0	23.0	41.0
34	34.04723610985211	36.0	33.0	40.0	22.0	41.0
35	33.76386282322805	36.0	33.0	40.0	21.0	41.0
36	33.531031497534926	36.0	33.0	40.0	19.0	41.0
37	33.41242378911602	35.0	33.0	40.0	18.0	41.0
38	33.30752699178395	35.0	32.0	40.0	18.0	41.0
39	33.25902614016513	35.0	32.0	40.0	18.0	41.0
40	33.14527484929145	35.0	32.0	40.0	18.0	41.0
41	33.04806235575937	35.0	32.0	40.0	18.0	41.0
42	32.98529889499911	35.0	32.0	40.0	18.0	41.0
43	32.87187766876344	35.0	32.0	40.0	17.0	41.0
44	32.73363783711809	35.0	31.0	40.0	17.0	41.0
45	32.65602667234557	35.0	31.0	40.0	17.0	41.0
46	32.55266916463286	35.0	31.0	39.0	17.0	41.0
47	32.52150342924581	35.0	31.0	39.0	17.0	41.0
48	32.397123276323086	35.0	31.0	39.0	17.0	41.0
49	32.28330736044143	35.0	31.0	39.0	15.0	41.0
50	32.13565136152747	35.0	31.0	39.0	15.0	41.0
51	31.816175682867303	35.0	30.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	4.0
12	11.0
13	17.0
14	36.0
15	99.0
16	329.0
17	874.0
18	1849.0
19	3644.0
20	6307.0
21	9928.0
22	14630.0
23	21520.0
24	30775.0
25	44179.0
26	59007.0
27	66614.0
28	67176.0
29	69729.0
30	75910.0
31	87844.0
32	105241.0
33	131390.0
34	197618.0
35	259622.0
36	196205.0
37	206617.0
38	275243.0
39	373114.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.35740231756555	22.62369725309018	27.39254574387938	19.626354685464882
2	36.72990520075173	23.77736207657066	27.4558262047034	12.03690651797421
3	26.506272312434586	23.972538275136852	36.91237326016684	12.608816152261722
4	23.954538692380194	25.217025089683464	37.16675290563144	13.661683312304906
5	21.61641457853435	29.954515271236364	35.569647759008575	12.859422391220715
6	20.175450390764436	37.88452421898075	32.29493812697643	9.645087263278379
7	80.91150754529497	3.536722835485115	13.807588363855277	1.7441812553646348
8	81.89805817031423	2.8609360910718165	13.407824136703145	1.8331816019108185
9	77.4243160917571	5.1999276546890645	14.747340073707207	2.628416179846626
10	41.24684627792484	28.252578819739167	20.4478296189857	10.0527452833503
11	33.75476934727441	21.52017102639693	30.219998273774955	14.505061352553708
12	31.351456383107458	21.124527185658973	32.00846283996983	15.515553591263739
13	22.123829322317878	29.184219874228457	32.63879521636149	16.053155587092174
14	16.5267397897909	32.87912217552933	34.124259577404494	16.469878457275282
15	15.894325533947864	27.007181182932577	42.959105381701754	14.139387901417802
16	19.708979276191236	24.690266215997596	40.619549975733094	14.981204532078078
17	19.9106179755544	24.773584766541074	32.318792995690075	22.997004262214453
18	21.35301345544713	26.67555513532433	35.471278954931215	16.50015245429732
19	23.68250644406749	28.381525228258564	30.921504903910417	17.014463423763527
20	24.908256343551745	28.257089558550476	30.648561833337745	16.18609226456004
21	21.54133680081922	28.907373279684457	33.0792862102811	16.472003709215223
22	21.001999905447974	25.061794953090484	33.86103194427156	20.075173197189983
23	18.795511294412886	28.748369736585865	32.632939930404504	19.823179038596745
24	19.564245281832264	26.566560071547258	36.99014013217332	16.87905451444716
25	18.161275394049902	28.19302839293219	35.109509027766634	18.536187185251272
26	17.39874367249607	32.496880433759586	31.470643981698544	18.633731912045796
27	17.115608067109385	31.832977751214536	34.058637002197685	16.992777179478395
28	15.907207163053233	29.327956301350316	37.63591311449599	17.128923421100456
29	17.504702662073232	27.635084699964303	36.738492953488645	18.12171968447382
30	18.60432536479516	29.663832852838446	34.78248046394683	16.94936131841956
31	24.04588115330917	27.77630552274909	31.205507959068512	16.97230536487323
32	24.806374367900194	28.42689285130306	30.860653302446337	15.906079478350405
33	23.611245445346544	29.530722685416304	30.23231606052891	16.625715808708243
34	18.84890282784288	30.480753675059386	31.6641286532105	19.006214843887236
35	18.9439753227889	30.488430605536326	31.903414672652648	18.664179399022125
36	23.782696892664802	29.771960466844117	29.596258515645975	16.849084124845106
37	19.738168960999026	32.69092894762295	30.240209853448697	17.33069223792933
38	19.183738439605328	33.2359476389969	28.204999199777586	19.37531472162019
39	19.603540756476924	31.71405038755487	30.20455766784394	18.477851188124266
40	21.720725413545836	29.290525843714178	28.659326017551113	20.329422725188877
41	18.151993681495863	28.37393504275877	30.58129110356526	22.892780172180103
42	20.366939927802154	29.484444240111827	28.36074980623341	21.787866025852605
43	20.303789584443848	30.30800105308402	28.72755094207214	20.66065842039999
44	19.3119908883076	31.991590941915998	29.37280345453197	19.32361471524443
45	18.63564030154289	34.417631090093764	27.19220821917333	19.754520389190013
46	20.646432244148944	32.497617766065275	27.974951520400896	18.880998469384878
47	19.59994083992559	30.581421221030975	29.109402331444752	20.709235607598686
48	20.54619842306306	29.16643715391465	30.822528884993076	19.464835538029217
49	19.87817535410384	28.11257242663435	31.16521491718674	20.84403730207507
50	18.46553340137031	31.593778476749527	30.126790795837454	19.813897326042706
51	17.68001426087424	33.13848965717951	28.78024851568501	20.401247566261237
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1738.0
1	2780.0
2	3822.0
3	41548.0
4	79274.0
5	52487.5
6	25701.0
7	26093.0
8	26485.0
9	29825.5
10	33166.0
11	35801.0
12	38436.0
13	38607.5
14	38779.0
15	35708.5
16	32638.0
17	28842.0
18	25046.0
19	23085.5
20	21125.0
21	19529.0
22	17933.0
23	17283.0
24	16633.0
25	18147.0
26	21877.5
27	24094.0
28	27899.0
29	31704.0
30	36758.5
31	41813.0
32	46741.0
33	51669.0
34	57361.0
35	63053.0
36	69163.0
37	75273.0
38	85548.0
39	95823.0
40	111976.0
41	128129.0
42	147556.5
43	166984.0
44	171332.0
45	175680.0
46	177560.5
47	179441.0
48	173150.5
49	166860.0
50	158223.5
51	149587.0
52	136808.0
53	124029.0
54	114889.0
55	105749.0
56	96555.0
57	87361.0
58	80680.0
59	73999.0
60	66164.5
61	58330.0
62	51123.5
63	43917.0
64	37730.5
65	31544.0
66	26067.5
67	20591.0
68	16335.0
69	12079.0
70	9707.5
71	7336.0
72	5900.5
73	4465.0
74	3579.5
75	2000.0
76	1306.0
77	1120.5
78	935.0
79	665.5
80	396.0
81	270.5
82	145.0
83	103.0
84	61.0
85	42.0
86	23.0
87	30.5
88	38.0
89	33.5
90	29.0
91	30.5
92	32.0
93	17.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2305609.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.167908067569513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.89501164271326	20.906853197239165
2	6.997963559788833	3.662441341855114
3	2.8808793794888135	2.2615976026865994
4	1.681755245920961	1.7603206666964986
5	1.0584543703653688	1.3848768328719074
6	0.7628245112911076	1.197691300989261
7	0.5685338698245359	1.0414139427207592
8	0.4148129115271478	0.86838289072666
9	0.3441224227208224	0.8104467529572995
>10	3.6254734166678904	22.381607659488132
>50	1.3087487349786944	24.4427862177825
>100	0.4578886864751431	15.738055312882892
>500	0.002186010813662487	0.3747698166665319
>1k	0.0011770827458182623	0.5735293871588092
>5k	0.0	0.0
>10k+	1.6815467797403748E-4	2.5952270772778427
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58979	2.5580660033856564	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT	2732	0.11849363877396385	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT	2719	0.1179297964225504	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2309	0.10014707610874177	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02997038960205308	0.0
2	0.0	0.0	0.0	0.09971335122303912	0.0
3	0.0	0.0	0.0	0.19053534228917393	0.0
4	0.0	0.0	0.0	0.2795356888353576	0.0
5	0.0	0.0	0.0	0.5372983884084422	0.0
6	0.0	0.0	0.0	1.0170848569727131	0.0
7	0.0	0.0	0.0	1.4234417023875254	0.0
8	0.0	0.0	0.0	1.9989946257149414	0.0
9	0.0	0.0	0.0	2.348446766125566	0.0
10	0.0	0.0	0.0	2.7136864923757673	0.0
11	0.0	0.0	0.0	2.995390805639638	0.0
12	0.0	0.0	0.0	3.2123399934681034	0.0
13	0.0	0.0	0.0	3.3221157620394437	0.0
14	0.0	0.0	0.0	3.3814493264035663	0.0
15	0.0	0.0	0.0	3.4403491658819862	0.0
16	0.0	0.0	0.0	3.537243305347958	0.0
17	0.0	0.0	0.0	3.650705735447771	0.0
18	0.0	0.0	0.0	3.8075840266064196	0.0
19	0.0	0.0	0.0	3.8979722927868514	0.0
20	0.0	0.0	0.0	3.995126667184245	0.0
21	0.0	0.0	0.0	4.1093264295897525	0.0
22	0.0	0.0	0.0	4.235670488794934	0.0
23	0.0	0.0	0.0	4.379233425962512	0.0
24	0.0	0.0	0.0	4.486536962685347	0.0
25	0.0	0.0	0.0	4.585816589022683	0.0
26	0.0	0.0	0.0	4.684835980428598	0.0
27	0.0	0.0	0.0	4.7866312111030105	0.0
28	0.0	0.0	0.0	4.89952979885141	0.0
29	0.0	0.0	0.0	5.019845082145324	0.0
30	0.0	0.0	0.0	5.177807685518229	0.0
31	0.0	0.0	0.0	5.30874922851186	0.0
32	0.0	0.0	0.0	5.440428103811184	0.0
33	0.0	0.0	0.0	5.573451526256187	0.0
34	0.0	0.0	0.0	5.717578305775177	0.0
35	0.0	0.0	0.0	5.879748040539398	0.0
36	0.0	0.0	0.0	6.023310977706974	0.0
37	0.0	0.0	0.0	6.185957809845468	0.0
38	0.0	0.0	0.0	6.377013621997485	0.0
39	0.0	0.0	0.0	6.627533115979336	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACG	35	1.2126475E-7	45.000004	1
CGAATCG	20	7.034767E-4	45.0	23
CCGACGT	20	7.034767E-4	45.0	43
AATCGTC	30	2.1664564E-6	44.999996	35
CGTTTTT	23900	0.0	44.143307	1
CGACGGT	395	0.0	41.58228	28
GACCGAT	520	0.0	41.53846	9
TATAGCG	240	0.0	41.249996	1
CACGACG	405	0.0	40.0	26
TCACGAC	410	0.0	39.512196	25
CTCACGA	430	0.0	39.244183	24
AGGGCGA	1365	0.0	39.230766	6
ATTAGCG	150	0.0	39.0	1
CGTTAGG	360	0.0	38.750004	2
GTTTTTT	27920	0.0	38.60942	2
TAGGGAC	1995	0.0	38.458645	5
GTTAGCG	130	0.0	38.07692	1
TACGGGA	615	0.0	38.04878	4
GTAGGGC	750	0.0	37.8	4
ATAGCGG	405	0.0	37.777775	2
>>END_MODULE