FastQCFastQC Report
Thu 26 May 2016
SRR1548139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548139_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2211726
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85510.3866211275718602No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32160.14540679993814784No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA253.89198E-545.022
GTCGATC207.034704E-445.029
CGACATA351.2126111E-745.015
ACTCGCG207.034704E-445.039
CGACTCG253.89198E-545.036
TATCCGC207.034704E-445.028
TGCCCCG302.1664164E-644.99999626
ATTCGAC302.1664164E-644.99999612
CGACGGT3700.042.56756628
CGTTTTT55050.041.771121
TATCGCT650.041.5384611
AGGGCGA13300.040.7706766
GCGATAC1050.040.7142879
TACGGGA9700.040.3608254
TCTAGCG1450.040.344831
TGATCGG451.9297659E-840.0000042
ATTACGC451.9297659E-840.00000442
TACGTCA451.9297659E-840.00000419
CACGACG3950.039.87341726
CGCATGG2150.039.767442