##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548139_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2211726
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15383958049053	31.0	31.0	33.0	30.0	34.0
2	31.539066321958508	31.0	31.0	34.0	30.0	34.0
3	31.672415570463972	31.0	31.0	34.0	30.0	34.0
4	35.47400310888419	37.0	35.0	37.0	33.0	37.0
5	35.32147426941673	37.0	35.0	37.0	33.0	37.0
6	35.36893132331944	37.0	35.0	37.0	33.0	37.0
7	35.77171901040183	37.0	35.0	37.0	35.0	37.0
8	35.76743683439992	37.0	35.0	37.0	35.0	37.0
9	37.49488905949471	39.0	37.0	39.0	35.0	39.0
10	36.82100856977763	39.0	37.0	39.0	32.0	39.0
11	36.49087409561582	39.0	35.0	39.0	32.0	39.0
12	35.74583741385687	37.0	35.0	39.0	31.0	39.0
13	35.38989684979062	37.0	35.0	39.0	30.0	39.0
14	36.27682995090712	38.0	35.0	40.0	30.0	41.0
15	36.609943998488056	38.0	35.0	40.0	31.0	41.0
16	36.75748080910565	38.0	35.0	40.0	31.0	41.0
17	36.649948953893926	38.0	35.0	40.0	31.0	41.0
18	36.60266145083071	38.0	35.0	40.0	31.0	41.0
19	36.5516298130962	38.0	35.0	40.0	31.0	41.0
20	36.42661568385957	38.0	35.0	40.0	31.0	41.0
21	36.278479341473584	38.0	35.0	40.0	30.0	41.0
22	36.17554073153727	38.0	34.0	40.0	30.0	41.0
23	36.17737866263723	38.0	34.0	40.0	30.0	41.0
24	36.03052548100443	38.0	34.0	40.0	30.0	41.0
25	35.92882753107754	37.0	34.0	40.0	30.0	41.0
26	35.88826870959603	37.0	34.0	40.0	30.0	41.0
27	35.87632373992077	37.0	34.0	40.0	30.0	41.0
28	35.907998549549085	37.0	34.0	40.0	30.0	41.0
29	35.97251467858134	38.0	35.0	40.0	30.0	41.0
30	35.9314874446473	37.0	35.0	40.0	30.0	41.0
31	35.77661654291716	37.0	34.0	40.0	30.0	41.0
32	35.641896871493124	37.0	34.0	40.0	29.0	41.0
33	35.540438553419364	37.0	34.0	40.0	29.0	41.0
34	35.43566382092538	37.0	34.0	40.0	29.0	41.0
35	35.303461188230365	37.0	34.0	40.0	28.0	41.0
36	35.1335518052417	37.0	34.0	40.0	27.0	41.0
37	35.04877457695935	37.0	34.0	40.0	27.0	41.0
38	34.98414903111868	37.0	34.0	40.0	27.0	41.0
39	34.97013825401519	37.0	34.0	40.0	27.0	41.0
40	34.84321746907167	37.0	34.0	40.0	26.0	41.0
41	34.79708924161492	37.0	34.0	40.0	26.0	41.0
42	34.75200137810922	37.0	33.0	40.0	26.0	41.0
43	34.67025255388778	36.0	33.0	40.0	26.0	41.0
44	34.544955839918686	36.0	33.0	40.0	25.0	41.0
45	34.462278781368035	36.0	33.0	40.0	25.0	41.0
46	34.37096683766434	36.0	33.0	40.0	24.0	41.0
47	34.33320040547518	36.0	33.0	40.0	24.0	41.0
48	34.238672873583795	36.0	33.0	40.0	24.0	41.0
49	34.14976674325843	36.0	33.0	40.0	24.0	41.0
50	33.997955442943656	35.0	33.0	40.0	24.0	41.0
51	33.624433587162244	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	1.0
11	4.0
12	4.0
13	10.0
14	24.0
15	61.0
16	218.0
17	501.0
18	1017.0
19	1966.0
20	3386.0
21	5461.0
22	8252.0
23	12211.0
24	17485.0
25	24169.0
26	32258.0
27	39720.0
28	45823.0
29	53878.0
30	66208.0
31	81399.0
32	102002.0
33	131749.0
34	211529.0
35	272883.0
36	169226.0
37	211414.0
38	298351.0
39	420448.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.94380678257614	23.610790848414315	29.826117701740635	12.619284667268913
2	29.774935954996234	25.555425943358262	30.737623014785736	13.932015086859764
3	28.999387808435586	25.172195832576005	32.11265771619088	13.715758642797526
4	27.03612472792742	27.59455737283913	30.426327673500243	14.942990225733205
5	23.889803709862793	32.437652765306375	29.42855489332765	14.243988631503179
6	22.641683463503163	40.85352344729862	25.909628950421528	10.595164138776685
7	88.95658865519508	3.787901394657385	5.691075657653796	1.5644342924937358
8	90.28197887080044	2.787144519709946	5.352154832922342	1.57872177656726
9	85.21186620765863	5.81016816730463	6.554157250943381	2.423808374093355
10	46.39946358635744	32.58563673800462	12.078575736777521	8.936323938860419
11	39.94468573412801	20.925150764606464	24.061208305187893	15.068955196077633
12	37.13665255099411	21.22279161161916	26.481535235377258	15.159020602009472
13	24.407408512627697	33.4223136138925	26.21391619034184	15.956361683137965
14	17.10596158837035	37.32713726745537	28.901455243551872	16.665445900622412
15	15.773924979857359	26.804224393075813	43.07807567483495	14.343774952231877
16	20.81867283741295	22.92865391101791	40.08638502237619	16.166288229192947
17	20.80931363107365	22.766111172902974	28.726117068750828	27.698458127272545
18	23.13537029451207	25.85519182755911	32.47364275683335	18.535795121095468
19	27.088617667830462	26.32532239526958	28.01671635636602	18.569343580533936
20	29.709511937735506	26.650995647742988	26.803003627031558	16.83648878748995
21	24.883055134315914	26.50215261745804	30.05684248410517	18.557949764120874
22	23.342357959349393	23.40167814638884	29.716022689971545	23.539941204290223
23	20.209465367771596	27.176422395902566	29.131727890344468	23.482384345981373
24	21.18137599322882	24.595632551229222	36.58382638717454	17.639165068367422
25	18.987388130356113	26.462816822698652	33.52481274805288	21.024982298892358
26	18.282599200805162	33.39812436079333	28.278186357622957	20.04109008077854
27	18.129686950372694	32.628680044453965	31.13803427730198	18.103598727871358
28	16.383629798627858	28.662818088678254	37.17738996602653	17.776162146667353
29	18.028046873799013	26.245430039706548	36.18820776172094	19.538315324773503
30	20.035574026800788	28.81383136970854	32.15493239216793	18.995662211322742
31	26.229424440459624	27.186776300500153	27.940395871821373	18.64340338721885
32	26.97133370046742	27.583480051326433	27.77663236766218	17.668553880543975
33	26.00177417998432	28.436840729819156	26.8512464925583	18.710138597638224
34	20.184281416414148	28.608833101387788	29.2593205487479	21.947564933450163
35	19.892608758951155	27.634933079413997	31.159465503412264	21.312992658222583
36	26.280696614318412	26.429630071717746	29.11129136249246	18.178381951471383
37	20.73453040747362	31.348412958928908	29.664072312754836	18.252984320842636
38	20.27344255120209	31.734808018714794	26.625178706584812	21.3665707234983
39	20.50439340135261	30.246377715865346	29.227444990925637	20.02178389185641
40	23.559111752540776	27.014783928931525	28.013958329377147	21.412145989150556
41	19.114664293859185	26.138590404055474	29.130235842957035	25.616509459128302
42	21.864778910226672	25.783166630947957	27.66038831211461	24.69166614671076
43	21.94616331317713	26.142252702188244	27.711479631744616	24.200104352890005
44	19.480577612235876	30.39956124764098	29.146377082875546	20.9734840572476
45	18.898769558254504	34.11082566285336	26.121092757421128	20.869312021471014
46	21.860393195178787	30.8974077259118	27.453852782849232	19.78834629606018
47	21.14479822545831	28.608064470915473	28.1354471575593	22.111690146066916
48	22.31420166874197	26.042692449245518	31.083190232424812	20.559915649587698
49	21.420872205689133	25.114819828495932	31.753616858507787	21.710691107307145
50	19.60988838581271	31.145584941353494	29.006305482686372	20.238221190147424
51	19.37699335270282	32.13200911867021	26.972237971611314	21.518759557015652
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	835.0
1	1798.0
2	2761.0
3	8766.5
4	14772.0
5	10849.0
6	6926.0
7	7106.5
8	7287.0
9	7897.5
10	8508.0
11	8407.5
12	8307.0
13	8077.5
14	7848.0
15	7600.5
16	7353.0
17	7187.0
18	7021.0
19	6897.0
20	6773.0
21	7241.5
22	7710.0
23	10186.5
24	12663.0
25	15507.0
26	21919.5
27	25488.0
28	31178.0
29	36868.0
30	40028.5
31	43189.0
32	52945.5
33	62702.0
34	70318.5
35	77935.0
36	86310.0
37	94685.0
38	103764.0
39	112843.0
40	134961.0
41	157079.0
42	181048.5
43	205018.0
44	208815.5
45	212613.0
46	201186.0
47	189759.0
48	180094.0
49	170429.0
50	162979.0
51	155529.0
52	144580.5
53	133632.0
54	118548.5
55	103465.0
56	95180.5
57	86896.0
58	77683.0
59	68470.0
60	60635.5
61	52801.0
62	45401.0
63	38001.0
64	31194.0
65	24387.0
66	20481.5
67	16576.0
68	13593.5
69	10611.0
70	8479.0
71	6347.0
72	5618.5
73	4890.0
74	3474.0
75	1679.0
76	1300.0
77	926.5
78	553.0
79	381.5
80	210.0
81	176.5
82	143.0
83	107.5
84	72.0
85	47.0
86	22.0
87	19.5
88	17.0
89	14.0
90	11.0
91	11.5
92	12.0
93	6.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2211726.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.007089889202515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.57462466632049	14.869286889335923
2	7.017006419901114	2.52711730712541
3	2.0658768982592046	1.1160129302094113
4	0.9817777204502793	0.7071583865345806
5	0.5694026842460628	0.5126642659186024
6	0.38052529160513876	0.411129187862924
7	0.2612830832762434	0.3293463576958311
8	0.2208932176290705	0.31821152206094766
9	0.18391057964936044	0.2980524905389235
>10	2.602810212216933	12.131981440315538
>50	1.534228013412615	20.287099714709782
>100	1.6023612310135569	45.45344585997803
>500	0.0047952218276232075	0.5037269087244719
>1k	2.5238009619069513E-4	0.1461553354797818
>5k	2.5238009619069513E-4	0.38861140350983026
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8551	0.3866211275718602	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3216	0.14540679993814784	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.521355719469772E-5	0.0	0.0	0.023511049741242813	0.0
2	4.521355719469772E-5	0.0	0.0	0.08201739275118165	0.0
3	4.521355719469772E-5	0.0	0.0	0.11520414373208979	0.0
4	4.521355719469772E-5	0.0	0.0	0.1724897206977718	0.0
5	4.521355719469772E-5	0.0	0.0	0.3078591109386967	0.0
6	4.521355719469772E-5	0.0	0.0	0.432558101681673	0.0
7	4.521355719469772E-5	0.0	0.0	0.5157058333627221	0.0
8	4.521355719469772E-5	0.0	0.0	0.7165896679787641	0.0
9	4.521355719469772E-5	0.0	0.0	0.7792556582506152	0.0
10	4.521355719469772E-5	0.0	0.0	0.9064866081964945	0.0
11	4.521355719469772E-5	0.0	0.0	1.0877477589900377	0.0
12	4.521355719469772E-5	0.0	0.0	1.237585487533266	0.0
13	4.521355719469772E-5	0.0	0.0	1.2988046439748866	0.0
14	4.521355719469772E-5	0.0	0.0	1.3222704801589347	0.0
15	4.521355719469772E-5	0.0	0.0	1.367257969567659	0.0
16	4.521355719469772E-5	0.0	0.0	1.4757252932777387	0.0
17	4.521355719469772E-5	0.0	0.0	1.6018259042937506	0.0
18	4.521355719469772E-5	0.0	0.0	1.7635547983791844	0.0
19	4.521355719469772E-5	0.0	0.0	1.850681323093367	0.0
20	4.521355719469772E-5	0.0	0.0	1.9492016642206131	0.0
21	4.521355719469772E-5	0.0	0.0	2.070373997502403	0.0
22	4.521355719469772E-5	0.0	0.0	2.2077779978170895	0.0
23	4.521355719469772E-5	0.0	0.0	2.35725401790276	0.0
24	4.521355719469772E-5	0.0	0.0	2.469428853302805	0.0
25	4.521355719469772E-5	0.0	0.0	2.564422536968865	0.0
26	4.521355719469772E-5	0.0	0.0	2.6620386069522173	0.0
27	4.521355719469772E-5	0.0	0.0	2.7661654291716062	0.0
28	4.521355719469772E-5	0.0	0.0	2.877662061213731	0.0
29	4.521355719469772E-5	0.0	0.0	2.9954433777059184	0.0
30	4.521355719469772E-5	0.0	0.0	3.1458688824926777	0.0
31	4.521355719469772E-5	0.0	0.0	3.2893767130286484	0.0
32	4.521355719469772E-5	0.0	0.0	3.4133522868565094	0.0
33	4.521355719469772E-5	0.0	0.0	3.5428891282193185	0.0
34	4.521355719469772E-5	0.0	0.0	3.686939521441625	0.0
35	4.521355719469772E-5	0.0	0.0	3.853415839032502	0.0
36	4.521355719469772E-5	0.0	0.0	4.010623377398466	0.0
37	9.042711438939544E-5	0.0	0.0	4.16859954623674	0.0
38	9.042711438939544E-5	0.0	0.0	4.3428978092223	0.0
39	9.042711438939544E-5	0.0	0.0	4.5820323132250556	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	25	3.89198E-5	45.0	22
GTCGATC	20	7.034704E-4	45.0	29
CGACATA	35	1.2126111E-7	45.0	15
ACTCGCG	20	7.034704E-4	45.0	39
CGACTCG	25	3.89198E-5	45.0	36
TATCCGC	20	7.034704E-4	45.0	28
TGCCCCG	30	2.1664164E-6	44.999996	26
ATTCGAC	30	2.1664164E-6	44.999996	12
CGACGGT	370	0.0	42.567566	28
CGTTTTT	5505	0.0	41.77112	1
TATCGCT	65	0.0	41.53846	11
AGGGCGA	1330	0.0	40.770676	6
GCGATAC	105	0.0	40.714287	9
TACGGGA	970	0.0	40.360825	4
TCTAGCG	145	0.0	40.34483	1
TGATCGG	45	1.9297659E-8	40.000004	2
ATTACGC	45	1.9297659E-8	40.000004	42
TACGTCA	45	1.9297659E-8	40.000004	19
CACGACG	395	0.0	39.873417	26
CGCATGG	215	0.0	39.76744	2
>>END_MODULE