FastQCFastQC Report
Thu 26 May 2016
SRR1548138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548138_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences279203
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39371.4100851351883754No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG8910.3191226455303131No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC7620.27291970358484685No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGC5410.19376582629842803No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3160.11317929964935908No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG207.024102E-445.0000041
TTGTGCG207.024102E-445.0000041
GGCACCG406.7830115E-945.0000048
GCACCGA406.7830115E-945.0000049
CGTTAGG351.2075179E-745.0000042
TACGAAG207.024102E-445.0000041
GAACGCT207.024102E-445.00000430
TTAACGG207.024102E-445.0000042
ATTGCGG453.8198777E-1045.0000042
AACACGT207.024102E-445.00000441
CGGGCAA253.8831953E-545.06
TATTGCG302.1595788E-644.9999961
TTGCGAG302.1595788E-644.9999961
CGTTTTT17250.043.434781
CGGGATC800.042.1875046
CGGGCGA650.041.5384646
TCACGAC556.002665E-1140.90909225
GACCGAT556.002665E-1140.9090929
CGACGGT501.0750227E-940.528
ATGATCG403.4456934E-739.37500427