##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548138_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	279203
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.032270426893692	31.0	31.0	33.0	30.0	34.0
2	31.403992793773707	31.0	31.0	34.0	30.0	34.0
3	31.431420865821643	31.0	31.0	34.0	30.0	34.0
4	35.290738280032805	37.0	35.0	37.0	33.0	37.0
5	35.17155976117735	37.0	35.0	37.0	32.0	37.0
6	35.21838590559557	37.0	35.0	37.0	32.0	37.0
7	35.62599255738656	37.0	35.0	37.0	35.0	37.0
8	35.600631082044245	37.0	35.0	37.0	35.0	37.0
9	37.32780450066797	39.0	37.0	39.0	35.0	39.0
10	36.672313692904446	39.0	35.0	39.0	32.0	39.0
11	36.33855295251126	38.0	35.0	39.0	32.0	39.0
12	35.82739440478792	37.0	35.0	39.0	31.0	39.0
13	35.57993646200077	37.0	35.0	39.0	30.0	39.0
14	36.56752613689681	38.0	35.0	40.0	31.0	41.0
15	36.78943277830109	38.0	35.0	41.0	31.0	41.0
16	36.9143705475944	38.0	35.0	41.0	31.0	41.0
17	36.73041478780672	38.0	35.0	40.0	31.0	41.0
18	36.6253084673159	38.0	35.0	40.0	31.0	41.0
19	36.512566125722145	38.0	35.0	40.0	31.0	41.0
20	36.325372578374875	38.0	35.0	40.0	30.0	41.0
21	36.24179897780468	38.0	35.0	40.0	30.0	41.0
22	36.14244116288149	38.0	34.0	40.0	30.0	41.0
23	36.09164299810532	38.0	34.0	40.0	30.0	41.0
24	35.90908765306963	38.0	34.0	40.0	30.0	41.0
25	35.84140571555463	37.0	34.0	40.0	30.0	41.0
26	35.724648374122054	37.0	34.0	40.0	29.0	41.0
27	35.64721367607081	37.0	34.0	40.0	29.0	41.0
28	35.6273141764236	37.0	34.0	40.0	29.0	41.0
29	35.618582178558256	37.0	34.0	40.0	29.0	41.0
30	35.53941755640162	37.0	34.0	40.0	29.0	41.0
31	35.399863181985864	37.0	34.0	40.0	28.0	41.0
32	35.16575036801181	37.0	34.0	40.0	27.0	41.0
33	34.9928116818229	37.0	34.0	40.0	25.0	41.0
34	34.79302156495453	37.0	34.0	40.0	25.0	41.0
35	34.57405901799049	37.0	33.0	40.0	24.0	41.0
36	34.39851649158498	37.0	33.0	40.0	23.0	41.0
37	34.3336031489633	37.0	33.0	40.0	23.0	41.0
38	34.22646246637751	37.0	33.0	40.0	23.0	41.0
39	34.14210449028126	37.0	33.0	40.0	23.0	41.0
40	33.981826842834785	36.0	33.0	40.0	22.0	41.0
41	33.89035934427638	36.0	33.0	40.0	22.0	41.0
42	33.79640619907379	36.0	33.0	40.0	21.0	41.0
43	33.65968130714928	36.0	33.0	40.0	21.0	41.0
44	33.47933940537888	36.0	32.0	40.0	20.0	41.0
45	33.39382098329889	35.0	32.0	40.0	20.0	41.0
46	33.239542555058506	35.0	32.0	40.0	20.0	41.0
47	33.17405615269177	35.0	32.0	40.0	19.0	41.0
48	33.087438172225944	35.0	32.0	40.0	19.0	41.0
49	32.966458096796956	35.0	31.0	39.0	18.0	41.0
50	32.849428551985476	35.0	31.0	39.0	18.0	41.0
51	32.51522369028986	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	6.0
15	13.0
16	40.0
17	77.0
18	196.0
19	414.0
20	625.0
21	1180.0
22	1637.0
23	2266.0
24	3247.0
25	4451.0
26	5713.0
27	6590.0
28	7201.0
29	7865.0
30	9087.0
31	10881.0
32	12905.0
33	15841.0
34	23532.0
35	29830.0
36	23403.0
37	26401.0
38	35485.0
39	50309.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.04549019888755	23.223604330899022	27.717109056851108	16.01379641336232
2	32.10603037932974	26.88044182906344	28.274051496581343	12.739476295025485
3	27.54877275673972	26.688466814468327	32.95881491244722	12.80394551634474
4	24.067792967840603	26.824210341579423	34.834152928156215	14.273843762423757
5	23.885488336443377	30.859625433824135	30.957045590484345	14.297840639248147
6	21.116535280781367	39.97628965304814	28.790879754157366	10.116295312013122
7	83.82861215674616	3.33699852795278	10.850528110371307	1.9838612049297464
8	83.73405729881127	4.472014985512334	9.534997833117838	2.258929882558568
9	78.79284964703102	5.984892712470854	10.532838114203644	4.689419526294488
10	40.79289978975871	33.27829572031819	16.0961737517147	9.832630738208401
11	33.15902766087757	21.970394300920834	29.593521559582097	15.2770564786195
12	30.899381453637677	21.60291973940108	30.499672281458295	16.998026525502947
13	23.113648492315626	29.88721467892537	30.38720930649026	16.611927522268743
14	16.686067126785886	34.1489883704688	30.864997868934076	18.299946633811242
15	15.588657714995898	27.84174955140167	40.158236122104704	16.411356611497727
16	18.94141538593783	26.567766105665054	37.58018359401583	16.91063491438129
17	19.712538905384257	26.372209467663314	30.63398315920674	23.28126846774569
18	20.77126678438269	27.559517626959597	33.19591838196581	18.473297206691903
19	23.40841609868089	29.642231637912197	27.980000214897405	18.969352048509506
20	25.201376776037502	28.45814693968188	29.381489453909882	16.958986830370733
21	22.17096521169185	30.051611193289467	30.50468655422757	17.272737040791107
22	21.400916179267416	24.633331303746736	31.618929596028693	22.346822920957152
23	18.47795331712052	29.531201312306816	31.089207494188813	20.901637876383848
24	19.205381031006112	28.181287450349746	34.88680279223361	17.726528726410532
25	17.97401890380834	30.29945953302794	32.31483902393599	19.41168253922773
26	17.76879188260871	32.97672302948034	29.903331984255182	19.351153103655765
27	18.479385966483168	32.46061109658564	30.356765507533943	18.703237429397248
28	15.924972152878015	30.41156434565531	35.37282908851266	18.290634412954017
29	17.382334717033842	26.72141774980928	35.24603961991812	20.650207913238756
30	18.2515947178218	30.400461313094773	32.83274176853401	18.51520220054942
31	22.867232801939807	28.599262901902918	28.556999745704736	19.976504550452535
32	23.440292546999853	29.255058147656005	28.563804830177325	18.740844475166814
33	22.239016056417732	28.564521154858653	28.498261121836084	20.698201666887535
34	18.65954162383642	29.171248159940976	29.718520216473323	22.450689999749287
35	18.388412731954887	29.07311167859944	31.35209865223511	21.18637693721056
36	22.36616368735293	28.776553260530868	28.78443283202544	20.072850220090757
37	18.245505958030535	30.97495370751747	31.116069669738504	19.66347066471349
38	18.464701310516006	31.57236849174257	27.997192007249204	21.965738190492225
39	18.847935015024913	30.393656228622184	28.98786904152176	21.770539714831145
40	20.838601304427247	29.107137100962383	28.41695827050569	21.637303324104685
41	17.960408734863165	27.92376872741339	29.47103003907551	24.644792498647938
42	20.061030862848895	28.47820403075898	28.48679992693488	22.973965179457238
43	20.481513450786704	29.047682152412403	27.68200914746618	22.788795249334715
44	19.218633037610626	30.834195907637096	28.814518468641097	21.13265258611118
45	17.960766897203825	32.908672184754465	27.08853414898837	22.04202676905334
46	20.912382746603726	31.596365368566957	27.342829410858766	20.14842247397055
47	19.29169815510578	29.596745020648058	29.961354283442514	21.150202540803644
48	20.42384931394004	28.022263371095583	30.5713047495908	20.98258256537358
49	19.008391743641724	27.17771657181334	31.898654384086132	21.915237300458806
50	18.231179464404036	30.348885936039373	30.451678527809516	20.968256071747078
51	17.91026600717041	31.34063745733391	28.277991282328628	22.47110525316705
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	193.0
1	297.0
2	401.0
3	2934.0
4	5467.0
5	3682.0
6	1897.0
7	1938.0
8	1979.0
9	2258.0
10	2537.0
11	2746.5
12	2956.0
13	2993.5
14	3031.0
15	2788.0
16	2545.0
17	2234.0
18	1923.0
19	1767.0
20	1611.0
21	1648.5
22	1686.0
23	1815.0
24	1944.0
25	2184.5
26	3086.5
27	3748.0
28	4146.0
29	4544.0
30	5438.5
31	6333.0
32	7152.0
33	7971.0
34	8678.5
35	9386.0
36	10213.5
37	11041.0
38	11806.5
39	12572.0
40	15024.0
41	17476.0
42	19852.0
43	22228.0
44	21893.0
45	21558.0
46	21254.0
47	20950.0
48	22052.0
49	23154.0
50	21436.0
51	19718.0
52	18464.5
53	17211.0
54	15168.5
55	13126.0
56	11727.0
57	10328.0
58	9266.0
59	8204.0
60	7115.0
61	6026.0
62	5354.5
63	4683.0
64	3975.0
65	3267.0
66	2642.5
67	2018.0
68	1640.0
69	1262.0
70	1035.5
71	809.0
72	638.0
73	467.0
74	346.0
75	195.5
76	166.0
77	127.5
78	89.0
79	53.0
80	17.0
81	16.0
82	15.0
83	12.0
84	9.0
85	5.0
86	1.0
87	1.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	279203.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.80690984449645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.35437228662053	26.263339477642784
2	7.130431311185316	5.24898284846345
3	3.7373915204774373	4.126854982433955
4	2.6895942680904863	3.9598261497552385
5	2.2357076865667938	4.114474562905586
6	1.9588581406674608	4.325970898902311
7	1.6340467751276837	4.210094863367037
8	1.3152327059639557	3.8727721306358744
9	1.2262845933124564	4.062217182277192
>10	6.687098786657532	36.50661572706049
>50	0.012992420299821655	0.29796214041249725
>100	0.013991837245961784	0.7555731313669992
>500	0.002998250838420382	0.8070727605740975
>1k	9.994169461401273E-4	1.4482431442024712
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3937	1.4100851351883754	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG	891	0.3191226455303131	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC	762	0.27291970358484685	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGC	541	0.19376582629842803	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	316	0.11317929964935908	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.037248883428902986	0.0
2	0.0	0.0	0.0	0.18660257948517744	0.0
3	0.0	0.0	0.0	0.2997818791345365	0.0
4	0.0	0.0	0.0	0.505367062674828	0.0
5	0.0	0.0	0.0	1.2596569521101135	0.0
6	0.0	0.0	0.0	1.772187261598192	0.0
7	0.0	0.0	0.0	2.211652453591115	0.0
8	0.0	0.0	0.0	2.8212447573987385	0.0
9	0.0	0.0	0.0	3.076256343950459	0.0
10	0.0	0.0	0.0	3.7750310705830525	0.0
11	0.0	0.0	0.0	4.052606884596512	0.0
12	0.0	0.0	0.0	4.562988220040616	0.0
13	0.0	0.0	0.0	4.683688928843888	0.0
14	0.0	0.0	0.0	4.750665286547781	0.0
15	0.0	0.0	0.0	4.866351722581777	0.0
16	0.0	0.0	0.0	4.998155463945588	0.0
17	0.0	0.0	0.0	5.135331640419336	0.0
18	0.0	0.0	0.0	5.289341446904224	0.0
19	0.0	0.0	0.0	5.426517623377972	0.0
20	0.0	0.0	0.0	5.522146968334868	0.0
21	0.0	0.0	0.0	5.655025196720666	0.0
22	0.0	0.0	0.0	5.791485048513089	0.0
23	0.0	0.0	0.0	5.932959173074788	0.0
24	0.0	0.0	0.0	6.038975225910896	0.0
25	0.0	0.0	0.0	6.137828031933754	0.0
26	0.0	0.0	0.0	6.236680837956612	0.0
27	0.0	0.0	0.0	6.33983159206742	0.0
28	0.0	0.0	0.0	6.4462058072441915	0.0
29	0.0	0.0	0.0	6.559026944552888	0.0
30	0.0	0.0	0.0	6.708738802949825	0.0
31	0.0	0.0	0.0	6.834453784522372	0.0
32	0.0	0.0	0.0	6.948349408853057	0.0
33	0.0	0.0	0.0	7.060096059139766	0.0
34	0.0	0.0	0.0	7.190467151140926	0.0
35	0.0	0.0	0.0	7.338746360175213	0.0
36	0.0	0.0	0.0	7.468759289835711	0.0
37	0.0	0.0	0.0	7.6088007650347596	0.0
38	0.0	0.0	0.0	7.77033198067356	0.0
39	0.0	0.0	0.0	7.960516183565363	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	7.024102E-4	45.000004	1
TTGTGCG	20	7.024102E-4	45.000004	1
GGCACCG	40	6.7830115E-9	45.000004	8
GCACCGA	40	6.7830115E-9	45.000004	9
CGTTAGG	35	1.2075179E-7	45.000004	2
TACGAAG	20	7.024102E-4	45.000004	1
GAACGCT	20	7.024102E-4	45.000004	30
TTAACGG	20	7.024102E-4	45.000004	2
ATTGCGG	45	3.8198777E-10	45.000004	2
AACACGT	20	7.024102E-4	45.000004	41
CGGGCAA	25	3.8831953E-5	45.0	6
TATTGCG	30	2.1595788E-6	44.999996	1
TTGCGAG	30	2.1595788E-6	44.999996	1
CGTTTTT	1725	0.0	43.43478	1
CGGGATC	80	0.0	42.187504	6
CGGGCGA	65	0.0	41.538464	6
TCACGAC	55	6.002665E-11	40.909092	25
GACCGAT	55	6.002665E-11	40.909092	9
CGACGGT	50	1.0750227E-9	40.5	28
ATGATCG	40	3.4456934E-7	39.375004	27
>>END_MODULE