FastQCFastQC Report
Thu 26 May 2016
SRR1548137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548137_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4590882
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298700.6506375027717985No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT191250.042.4117661
CGGTCTA8350.040.6886231
ACGTTAG950.040.263161
CTCACGA9100.039.8076924
CGACGGT8500.039.44117428
AGGGCGA27150.039.281776
ACGGGAC20750.039.253015
CGTTAGG7350.039.1836782
AATGCGG8450.039.142012
TAAGGGA70050.038.2227024
GGCGATA12700.038.090558
AGGGATT109300.038.02156
TACGGGA25550.037.954994
GGGCGAT48450.037.9411777
TCTCACG9550.037.9319423
TATCGCG950.037.8947371
CGAGGGA39250.037.662424
TAGGGAC53300.037.3592875
GCGGGAT33900.037.3008845
CGGGATC14250.037.2631576
Produced by FastQC (version 0.11.5)