FastQCFastQC Report
Thu 26 May 2016
SRR1548136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548136_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1466515
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71290.486118450885262No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC47250.3221924085331551No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA302.1659125E-645.00000421
GCGCGTC351.2122291E-745.00000438
TTAGCCG207.033923E-445.00000415
GCGCGAA207.033923E-445.00000436
CGACAAC406.8175723E-945.00000431
CGTTCCA207.033923E-445.00000413
CGTTCAA207.033923E-445.00000436
TAGCCGT207.033923E-445.00000444
CCGTACT207.033923E-445.00000421
TACGCAA453.8562575E-1045.00000412
CGTAGGA207.033923E-445.00000438
TCGCTAG207.033923E-445.00000433
AACGCGA207.033923E-445.00000427
TCGTCGT351.2122291E-745.00000412
TTGCGTA207.033923E-445.00000423
TATTACG406.8175723E-945.0000041
GAACGAC207.033923E-445.0000049
CGTCGTG351.2122291E-745.00000413
ACGTATT302.1659125E-645.00000428
CCGAAAT207.033923E-445.00000415