##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548136_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1466515
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.175246758471616	31.0	31.0	33.0	30.0	34.0
2	31.552617600229116	31.0	31.0	34.0	30.0	34.0
3	31.64619864099583	31.0	31.0	34.0	30.0	34.0
4	35.44264054578371	37.0	35.0	37.0	33.0	37.0
5	35.290725290910764	37.0	35.0	37.0	33.0	37.0
6	35.33531876591784	37.0	35.0	37.0	33.0	37.0
7	35.80563171873455	37.0	35.0	37.0	35.0	37.0
8	35.81599642690324	37.0	35.0	37.0	35.0	37.0
9	37.58733118992987	39.0	37.0	39.0	35.0	39.0
10	36.84879936447974	39.0	37.0	39.0	32.0	39.0
11	36.4232537682874	38.0	35.0	39.0	32.0	39.0
12	35.37305175876142	37.0	35.0	39.0	30.0	39.0
13	34.90514996437132	37.0	34.0	39.0	29.0	39.0
14	35.700166721786005	37.0	34.0	40.0	27.0	41.0
15	36.15330630781138	37.0	35.0	40.0	30.0	41.0
16	36.3808157434462	37.0	35.0	40.0	31.0	41.0
17	36.28093609680092	37.0	35.0	40.0	31.0	41.0
18	36.22524420138901	37.0	35.0	40.0	31.0	41.0
19	36.119611459821414	37.0	35.0	40.0	31.0	41.0
20	35.96092436831536	36.0	34.0	40.0	30.0	41.0
21	35.79423326730378	36.0	34.0	40.0	30.0	41.0
22	35.70151277007054	36.0	34.0	40.0	30.0	41.0
23	35.721710995114265	36.0	34.0	40.0	30.0	41.0
24	35.56283570232831	36.0	34.0	40.0	30.0	41.0
25	35.45682314875743	35.0	34.0	40.0	30.0	41.0
26	35.41019559977225	35.0	34.0	40.0	30.0	41.0
27	35.384080626519335	36.0	34.0	40.0	29.0	41.0
28	35.471140765692816	36.0	34.0	40.0	30.0	41.0
29	35.592870171801856	36.0	34.0	40.0	30.0	41.0
30	35.53678346283536	36.0	34.0	40.0	30.0	41.0
31	35.313427411243666	36.0	34.0	40.0	29.0	41.0
32	35.09822743033654	35.0	34.0	40.0	29.0	41.0
33	34.990273539650126	35.0	34.0	40.0	28.0	41.0
34	34.92031380517758	35.0	34.0	40.0	27.0	41.0
35	34.795232234242405	35.0	34.0	40.0	27.0	41.0
36	34.56528300085577	35.0	33.0	40.0	25.0	41.0
37	34.44251916959595	35.0	33.0	40.0	25.0	41.0
38	34.432847260341696	35.0	33.0	40.0	25.0	41.0
39	34.4751543625534	35.0	33.0	40.0	25.0	41.0
40	34.35498511777922	35.0	33.0	40.0	24.0	41.0
41	34.3630695901508	35.0	33.0	40.0	25.0	41.0
42	34.29681046562769	35.0	33.0	40.0	25.0	41.0
43	34.1810039447261	35.0	33.0	40.0	24.0	41.0
44	34.0657504355564	35.0	33.0	40.0	24.0	41.0
45	33.968471512395034	35.0	33.0	40.0	24.0	41.0
46	33.868834618125284	35.0	33.0	39.0	23.0	41.0
47	33.85241473834226	35.0	33.0	39.0	23.0	41.0
48	33.79531542466323	35.0	33.0	39.0	23.0	41.0
49	33.75139974701929	35.0	33.0	39.0	24.0	41.0
50	33.56987483932998	35.0	33.0	39.0	24.0	41.0
51	33.16645994074387	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	0.0
13	3.0
14	15.0
15	50.0
16	120.0
17	322.0
18	696.0
19	1395.0
20	2494.0
21	3977.0
22	6150.0
23	9053.0
24	13074.0
25	18500.0
26	24519.0
27	29313.0
28	33467.0
29	38899.0
30	46561.0
31	56908.0
32	71859.0
33	93401.0
34	160103.0
35	230465.0
36	98902.0
37	117822.0
38	165041.0
39	243348.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.330739883328846	24.22477778952142	30.775546107608854	13.668936219540884
2	29.279141365754867	25.262476006041535	32.194761049153946	13.263621579049653
3	27.691840860816292	25.27352260290553	33.18213587996031	13.85250065631787
4	25.108232783162805	27.3189159333522	32.74627262591927	14.826578657565726
5	23.299659396596695	31.63506680804492	31.580583901289792	13.484689894068591
6	21.906697169820973	40.00197747721639	27.43851921050927	10.652806142453368
7	88.80447864495078	3.5000664841477924	6.161614439675011	1.533840431226411
8	89.88118089484254	2.517055740991398	5.6665632468812115	1.9352001172848556
9	86.45073524648572	4.2006389297075035	7.05822988513585	2.290395938670931
10	53.11858385355759	25.107619083337028	12.302840407360307	9.470956655745082
11	46.106790588572224	20.024207048683444	21.82016549438635	12.048836868357977
12	42.16097346430142	21.17271217819115	22.870546840639204	13.79576751686822
13	21.100022843271294	41.37734697565316	24.001663808416552	13.520966372658991
14	13.756627105757527	44.683347937116224	27.73316331575197	13.826861641374277
15	12.7091778808945	25.61869466047057	48.876076957958155	12.796050500676776
16	15.145020678274685	21.23817349294075	47.102416272591825	16.514389556192743
17	16.153056736548894	20.50002898026955	28.348090541180966	34.998823742000596
18	20.752123230925015	24.22750534430265	35.54508477581205	19.475286648960292
19	27.774758526165773	25.62946850185644	26.72294521365278	19.87282775832501
20	30.130411212977705	24.65818624425935	26.527106780360242	18.6842957624027
21	21.220648953471326	29.04927668656645	29.990828597048104	19.73924576291412
22	22.359403074636127	23.43644626887553	28.19746132838737	26.006689328100975
23	17.293311012843375	30.704697872166324	26.999246512991686	25.002744601998618
24	19.313815405911296	24.192456265363806	39.31790673808314	17.17582159064176
25	14.661561593301126	26.657688465511775	37.20166517219394	21.479084768993157
26	15.028895033463687	36.19812957930877	28.579182620020934	20.19379276720661
27	16.389331169473206	36.310436647426044	30.24244552561685	17.0577866574839
28	13.625295343041156	30.78188767247522	39.69451386450189	15.898303119981724
29	14.297978540962758	24.768447646290696	40.152947634357645	20.780626178388903
30	17.600911003296932	31.202749375219486	33.428161321227535	17.76817830025605
31	28.434349461137458	28.174959001442197	25.740207225974505	17.650484311445844
32	28.040626928466466	28.276424039304064	27.973665458587192	15.709283573642274
33	26.241054472678428	29.566830206305422	26.33808723402079	17.85402808699536
34	18.061322250369074	30.535316720251753	28.751973215412047	22.651387813967126
35	17.700193997333816	28.25467178992373	32.408669532872146	21.636464679870304
36	28.56152170281245	24.184955489715414	29.440271664456212	17.813251143015925
37	17.81700153084012	32.95397592251017	30.88730766476988	18.34171488187983
38	18.172811052051973	33.035666188208104	25.80184996403037	22.989672795709556
39	18.115191457298423	31.509940232455858	30.141935131928417	20.232933178317303
40	22.733487212882242	27.19508494628422	26.84323037950515	23.22819746132839
41	16.550120523826894	24.710282540580902	29.39649441021742	29.343102525374782
42	21.902401271040528	24.93735147611855	26.65073320081963	26.509514052021288
43	22.33335492647535	25.84794563983321	27.22147403879265	24.597225394898793
44	18.246591408884328	31.656205357599482	28.821934995550674	21.275268237965516
45	16.674837966198776	38.52602939622166	24.07469408768407	20.724438549895503
46	22.345901678469023	33.494031769194315	26.227007565555077	17.93305898678159
47	20.491982693664912	29.43365734411172	26.63191307282912	23.442446889394244
48	22.49087121509156	25.30441216080299	32.02735737445577	20.17735924964968
49	21.79268537996543	22.978898954323686	32.756500956348894	22.47191470936199
50	18.955960218613516	32.92833690756658	28.326815613887348	19.78888725993256
51	17.187822831679185	34.20265050135866	25.088730766476985	23.520795900485165
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	753.0
1	1238.5
2	1724.0
3	6300.0
4	10876.0
5	7963.0
6	5050.0
7	5359.5
8	5669.0
9	6364.0
10	7059.0
11	7327.0
12	7595.0
13	7619.5
14	7644.0
15	7285.5
16	6927.0
17	6384.5
18	5842.0
19	5364.0
20	4886.0
21	5250.0
22	5614.0
23	6600.0
24	7586.0
25	8740.0
26	11579.5
27	13265.0
28	16218.0
29	19171.0
30	21968.5
31	24766.0
32	27484.5
33	30203.0
34	35326.5
35	40450.0
36	43350.0
37	46250.0
38	56910.5
39	67571.0
40	92891.5
41	118212.0
42	144229.5
43	170247.0
44	180540.5
45	190834.0
46	176339.5
47	161845.0
48	145749.0
49	129653.0
50	115108.5
51	100564.0
52	87928.0
53	75292.0
54	65783.5
55	56275.0
56	49327.5
57	42380.0
58	36349.5
59	30319.0
60	27277.0
61	24235.0
62	19666.0
63	15097.0
64	12415.0
65	9733.0
66	7536.5
67	5340.0
68	4199.5
69	3059.0
70	2580.5
71	2102.0
72	1646.5
73	1191.0
74	862.5
75	462.0
76	390.0
77	258.5
78	127.0
79	129.0
80	131.0
81	112.5
82	94.0
83	65.0
84	36.0
85	26.0
86	16.0
87	12.0
88	8.0
89	4.5
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1466515.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.317635100747356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.58500556031251	16.37296737566126
2	8.445087576536224	3.431684155478356
3	2.686805350587386	1.6376859209991022
4	1.2321226258086853	1.0013527164222216
5	0.7168280063129358	0.7282124931131225
6	0.4688112840254328	0.5715081959964944
7	0.3074768462020417	0.4373041654143189
8	0.2550377049230883	0.4145410420447509
9	0.2043566410187081	0.3736839296369277
>10	2.418799954548755	12.149588608832083
>50	1.3487833529379332	20.249589957807775
>100	1.3258195610209123	41.236358821109754
>500	0.004390130996757967	0.5821824774743263
>1k	3.377023843659974E-4	0.3241970779099814
>5k	3.377023843659974E-4	0.4891430620995254
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7129	0.486118450885262	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4725	0.3221924085331551	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07555326744015574	0.0
2	0.0	0.0	0.0	0.277051376903748	0.0
3	0.0	0.0	0.0	0.4044963740568627	0.0
4	0.0	0.0	0.0	0.6152681697766473	0.0
5	0.0	0.0	0.0	1.0473810359934947	0.0
6	0.0	0.0	0.0	1.4344210594504658	0.0
7	0.0	0.0	0.0	1.687401765409832	0.0
8	0.0	0.0	0.0	2.3467881337729244	0.0
9	0.0	0.0	0.0	2.530829892636625	0.0
10	0.0	0.0	0.0	2.895435778017954	0.0
11	0.0	0.0	0.0	3.3861228831617813	0.0
12	0.0	0.0	0.0	3.7567293890618236	0.0
13	0.0	0.0	0.0	3.913768355591317	0.0
14	0.0	0.0	0.0	3.965523707565214	0.0
15	0.0	0.0	0.0	4.058942458822447	0.0
16	0.0	0.0	0.0	4.30176302322172	0.0
17	0.0	0.0	0.0	4.583314865514502	0.0
18	0.0	0.0	0.0	4.943147530028673	0.0
19	6.818886953082648E-5	0.0	0.0	5.114369781420579	0.0
20	6.818886953082648E-5	0.0	0.0	5.305298616106893	0.0
21	6.818886953082648E-5	0.0	0.0	5.559711288326406	0.0
22	6.818886953082648E-5	0.0	0.0	5.820329147673226	0.0
23	6.818886953082648E-5	0.0	0.0	6.114086797612026	0.0
24	6.818886953082648E-5	0.0	0.0	6.308834208992066	0.0
25	6.818886953082648E-5	0.0	0.0	6.481011104557403	0.0
26	6.818886953082648E-5	0.0	0.0	6.655029099600072	0.0
27	6.818886953082648E-5	0.0	0.0	6.813840976737367	0.0
28	6.818886953082648E-5	0.0	0.0	6.985063228129272	0.0
29	6.818886953082648E-5	0.0	0.0	7.16310436647426	0.0
30	6.818886953082648E-5	0.0	0.0	7.42126742651797	0.0
31	1.3637773906165297E-4	0.0	0.0	7.624674824328425	0.0
32	1.3637773906165297E-4	0.0	0.0	7.819831368925651	0.0
33	1.3637773906165297E-4	0.0	0.0	8.013828702740852	0.0
34	1.3637773906165297E-4	0.0	0.0	8.21907720002864	0.0
35	1.3637773906165297E-4	0.0	0.0	8.478876792941088	0.0
36	1.3637773906165297E-4	0.0	0.0	8.687057411618701	0.0
37	1.3637773906165297E-4	0.0	0.0	8.904170772204854	0.0
38	1.3637773906165297E-4	0.0	0.0	9.118556578009771	0.0
39	1.3637773906165297E-4	0.0	0.0	9.351762511805198	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	30	2.1659125E-6	45.000004	21
GCGCGTC	35	1.2122291E-7	45.000004	38
TTAGCCG	20	7.033923E-4	45.000004	15
GCGCGAA	20	7.033923E-4	45.000004	36
CGACAAC	40	6.8175723E-9	45.000004	31
CGTTCCA	20	7.033923E-4	45.000004	13
CGTTCAA	20	7.033923E-4	45.000004	36
TAGCCGT	20	7.033923E-4	45.000004	44
CCGTACT	20	7.033923E-4	45.000004	21
TACGCAA	45	3.8562575E-10	45.000004	12
CGTAGGA	20	7.033923E-4	45.000004	38
TCGCTAG	20	7.033923E-4	45.000004	33
AACGCGA	20	7.033923E-4	45.000004	27
TCGTCGT	35	1.2122291E-7	45.000004	12
TTGCGTA	20	7.033923E-4	45.000004	23
TATTACG	40	6.8175723E-9	45.000004	1
GAACGAC	20	7.033923E-4	45.000004	9
CGTCGTG	35	1.2122291E-7	45.000004	13
ACGTATT	30	2.1659125E-6	45.000004	28
CCGAAAT	20	7.033923E-4	45.000004	15
>>END_MODULE