FastQCFastQC Report
Thu 26 May 2016
SRR1548135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548135_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2132857
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311271.4594039825454777No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC58320.27343605314374103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCG351.2125747E-745.0000041
CCGATTC207.034645E-445.00000412
CTATCGT351.2125747E-745.00000413
GCGCGTC351.2125747E-745.00000438
TAGAACG351.2125747E-745.0000041
TATGCGT406.8193913E-945.00000420
CCGCTCG900.045.00000419
CCTCGCT207.034645E-445.00000443
CTAAACG351.2125747E-745.0000041
GTCCGTT406.8193913E-945.00000422
TCGTACT207.034645E-445.00000423
ACCGTAA351.2125747E-745.00000425
GCTACCG351.2125747E-745.0000041
TCGGTCA207.034645E-445.00000412
CGTATCA253.8919337E-545.020
ATCGTCC253.8919337E-545.037
TGCGCAC253.8919337E-545.012
GTATCCG253.8919337E-545.019
CCCGGTC253.8919337E-545.043
GTCGATC302.16638E-644.99999623