##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548135_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2132857
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09834649017726	31.0	31.0	33.0	30.0	34.0
2	31.460531578066416	31.0	31.0	34.0	30.0	34.0
3	31.443087839456652	31.0	31.0	34.0	30.0	34.0
4	35.301176778377545	37.0	35.0	37.0	33.0	37.0
5	35.172603695418864	37.0	35.0	37.0	32.0	37.0
6	35.2281156214411	37.0	35.0	37.0	32.0	37.0
7	35.750549614906205	37.0	35.0	37.0	35.0	37.0
8	35.774968504686434	37.0	35.0	37.0	35.0	37.0
9	37.54637183833703	39.0	37.0	39.0	35.0	39.0
10	36.74253501289585	39.0	37.0	39.0	32.0	39.0
11	36.369226816425105	38.0	35.0	39.0	32.0	39.0
12	35.43187611733932	37.0	35.0	39.0	30.0	39.0
13	34.958426186096865	37.0	34.0	39.0	28.0	39.0
14	35.843646339159164	38.0	34.0	40.0	28.0	41.0
15	36.268401960375215	38.0	35.0	40.0	30.0	41.0
16	36.484058706233	38.0	35.0	40.0	31.0	41.0
17	36.40335662447131	38.0	35.0	40.0	31.0	41.0
18	36.28517617449271	37.0	35.0	40.0	31.0	41.0
19	36.14043182454333	37.0	35.0	40.0	31.0	41.0
20	35.90253073694111	36.0	34.0	40.0	30.0	41.0
21	35.71484633053224	36.0	34.0	40.0	30.0	41.0
22	35.611156772348075	36.0	34.0	40.0	30.0	41.0
23	35.63320372626951	36.0	34.0	40.0	30.0	41.0
24	35.424340215963845	35.0	34.0	40.0	30.0	41.0
25	35.311389840012716	35.0	34.0	40.0	29.0	41.0
26	35.19062412529298	35.0	34.0	40.0	29.0	41.0
27	35.11055921705018	35.0	34.0	40.0	29.0	41.0
28	35.1588099905432	36.0	34.0	40.0	29.0	41.0
29	35.23287871620085	36.0	34.0	40.0	29.0	41.0
30	35.14729773257185	36.0	34.0	40.0	29.0	41.0
31	34.89142825796572	35.0	34.0	40.0	28.0	41.0
32	34.60678704667026	35.0	33.0	40.0	27.0	41.0
33	34.392843964691494	35.0	33.0	40.0	25.0	41.0
34	34.21749184310059	35.0	33.0	40.0	24.0	41.0
35	34.002040924450164	35.0	33.0	40.0	23.0	41.0
36	33.7407950931544	35.0	33.0	40.0	22.0	41.0
37	33.61797110636109	35.0	33.0	40.0	22.0	41.0
38	33.591645384571024	35.0	33.0	40.0	23.0	41.0
39	33.62732710162941	35.0	33.0	40.0	23.0	41.0
40	33.484134660692206	35.0	33.0	40.0	22.0	41.0
41	33.47227826338099	35.0	33.0	40.0	22.0	41.0
42	33.41466446179936	35.0	33.0	40.0	21.0	41.0
43	33.29601093744213	35.0	33.0	39.0	21.0	41.0
44	33.15803403603711	35.0	32.0	39.0	21.0	41.0
45	33.06619149807043	35.0	32.0	39.0	20.0	41.0
46	32.976555390258234	35.0	32.0	39.0	20.0	41.0
47	32.954408570288585	35.0	32.0	39.0	20.0	41.0
48	32.87145645488657	35.0	32.0	39.0	20.0	41.0
49	32.80793508425553	35.0	32.0	39.0	20.0	40.0
50	32.60715791072725	35.0	31.0	39.0	18.0	40.0
51	32.22833832741717	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	3.0
13	7.0
14	25.0
15	89.0
16	204.0
17	644.0
18	1484.0
19	2849.0
20	4953.0
21	8080.0
22	12272.0
23	18155.0
24	25769.0
25	36452.0
26	47421.0
27	53182.0
28	55480.0
29	61316.0
30	71113.0
31	85094.0
32	104134.0
33	134784.0
34	222909.0
35	318317.0
36	160948.0
37	169789.0
38	225852.0
39	311475.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.827222359492456	24.34063793306349	29.312607455633454	16.519532251810602
2	31.28296927548354	25.859258262508927	30.2986088612598	12.55916360074773
3	26.203397602370902	24.39483753481832	36.19590999302813	13.205854869782643
4	23.60748985984527	26.49966687874527	35.94399436999293	13.948848891416537
5	21.923832680765752	30.37381315296806	34.57798624099037	13.124367925275815
6	20.070637647062135	39.59852910907763	30.508702646262737	9.822130597597495
7	84.9106620837684	3.7113130416150732	9.95617615245654	1.421848722159995
8	86.5966635362802	2.3690758452160643	9.454736065287078	1.579524553216648
9	83.57142555736273	3.724581629241904	10.665647064008512	2.0383457493868553
10	50.26281649449541	20.893571392737535	17.43403331775173	11.409578795015324
11	43.05595733797437	23.762071249971285	21.76470340018107	11.417268011873276
12	38.79027051508845	22.789244661034473	25.231836921087535	13.188647902789544
13	19.737422621394686	41.58867659669635	25.61742301523262	13.056477766676341
14	13.664394753140973	44.07660710493015	29.338582005263365	12.920416136665514
15	11.915191688894286	26.208883202202493	50.30187208987756	11.574053019025655
16	13.469679401853945	22.171903695371984	48.58276949650164	15.77564740627243
17	14.809900523101174	22.528842768174332	30.049600137280652	32.611656571443845
18	19.299699886115196	26.585654828242117	36.45748402260442	17.65716126303826
19	26.16265413011749	28.692078278103033	27.543946921898655	17.60132066988082
20	27.688869905483582	27.01062471604988	27.96221218769003	17.3382931907765
21	18.7138190699142	31.246773693688795	31.71731625702051	18.32209097937649
22	19.725466826889942	26.477724479418917	28.944650297699283	24.852158395991854
23	16.534863800057856	32.75329757222355	27.733692413509203	22.978146214209392
24	17.610416450798155	26.578575122476565	40.383813823430266	15.427194603295016
25	14.838266231632034	27.49007551842435	38.08820750758255	19.583450742361066
26	13.7636981757333	38.302520984763625	29.841522427429503	18.09225841207357
27	15.127784000521366	37.66290942149427	31.556733526907806	15.65257305107656
28	12.581996823978352	31.858629059519693	40.88234701154367	14.67702710495828
29	12.944046412863122	26.917885259068004	40.771697305539	19.366371022529876
30	16.02714106008982	33.72373300225941	33.982915872934754	16.266210064716013
31	26.1886286797474	29.716338226144558	26.979727192212138	17.11530590189591
32	25.793149751718005	30.272259227880728	28.972265838731804	14.96232518166947
33	24.001234025534764	31.180571411960578	27.09211166055671	17.72608290194795
34	16.57668563809013	32.13970744405274	29.218742747404068	22.064864170453056
35	16.276337325943558	29.580464138008317	32.987256060767315	21.15594247528081
36	27.143966988879235	27.112835037698265	28.56084585136275	17.182352122059754
37	16.546022541595615	34.45852206688025	31.519881548552014	17.475573842972125
38	16.318112278507186	36.42105401346644	26.067148430485492	21.193685277540876
39	18.18438835796305	34.20205855338637	29.032748093285203	18.580804995365373
40	21.337764322690177	29.323156686078818	27.108896658331993	22.23018233289902
41	15.246357350727216	27.805427180537656	28.668260460030844	28.279955008704288
42	19.80029603484903	28.182573890326452	26.669017191494788	25.348112883329733
43	20.312238466995208	29.131535775722423	27.55402729765756	23.00219845962481
44	17.276732570444246	34.452848925174074	28.81332410002171	19.457094404359975
45	15.507978265772154	40.77150976366442	23.916324441816776	19.804187528746652
46	21.232834643860325	35.24080611123953	25.65193072015611	17.87442852474404
47	19.110141936379232	30.80431552607606	28.08463014632486	22.000912391219853
48	20.868956521698358	28.060952984658606	31.061576092536914	20.008514401106122
49	19.813799049819092	26.603049337109802	31.986485732517462	21.59666588055364
50	17.590818324904106	34.98893737367297	28.155474089449033	19.264770211973893
51	16.35552688248673	36.055581785370514	25.2814886323837	22.307402699759056
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2198.0
1	2584.0
2	2970.0
3	21495.5
4	40021.0
5	27337.5
6	14654.0
7	15928.0
8	17202.0
9	20367.0
10	23532.0
11	25770.5
12	28009.0
13	27912.5
14	27816.0
15	25882.5
16	23949.0
17	21465.0
18	18981.0
19	17458.5
20	15936.0
21	15502.0
22	15068.0
23	14654.5
24	14241.0
25	15930.5
26	19093.5
27	20567.0
28	23694.5
29	26822.0
30	29867.5
31	32913.0
32	40045.5
33	47178.0
34	54853.5
35	62529.0
36	63830.5
37	65132.0
38	80682.5
39	96233.0
40	127320.5
41	158408.0
42	194707.0
43	231006.0
44	232751.0
45	234496.0
46	232486.0
47	230476.0
48	210613.0
49	190750.0
50	171805.0
51	152860.0
52	128988.5
53	105117.0
54	89964.5
55	74812.0
56	61616.5
57	48421.0
58	41404.0
59	34387.0
60	29448.0
61	24509.0
62	20013.5
63	15518.0
64	12219.0
65	8920.0
66	6699.5
67	4479.0
68	3270.0
69	2061.0
70	1785.0
71	1509.0
72	1133.0
73	757.0
74	559.5
75	285.0
76	208.0
77	139.5
78	71.0
79	61.0
80	51.0
81	35.0
82	19.0
83	18.5
84	18.0
85	25.0
86	32.0
87	25.0
88	18.0
89	14.0
90	10.0
91	8.5
92	7.0
93	4.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2132857.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.829240687840816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.43420072412253	19.722908886254977
2	8.557896811647366	4.249721594361959
3	3.217925571289184	2.3969594557529046
4	1.71309913004802	1.7013980248837202
5	1.1036201398369705	1.3701025039980341
6	0.7612417446673148	1.1340632699985973
7	0.5362488748248374	0.9320256667126797
8	0.4067843078206626	0.8080116389532768
9	0.3236993590069708	0.7233488365755484
>10	2.4061324404571667	12.569013239333868
>50	0.6002508311122584	10.939886476430653
>100	0.9301448660453969	40.12108884782394
>500	0.007042225380175708	1.1346549042451672
>1k	0.0013323129097629715	0.4502231267281844
>5k	1.9033041568042452E-4	0.2756063057708378
>10k+	1.9033041568042452E-4	1.4709872221757319
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31127	1.4594039825454777	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5832	0.27343605314374103	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.377093729209225E-5	0.0	0.0	0.06671802188332364	0.0
2	9.377093729209225E-5	0.0	0.0	0.2216744957585061	0.0
3	9.377093729209225E-5	0.0	0.0	0.3575954693633938	0.0
4	9.377093729209225E-5	0.0	0.0	0.533697289597943	0.0
5	9.377093729209225E-5	0.0	0.0	0.9784059597056906	0.0
6	9.377093729209225E-5	0.0	0.0	1.5498460515637007	0.0
7	9.377093729209225E-5	0.0	0.0	1.9712526437543634	0.0
8	9.377093729209225E-5	0.0	0.0	2.759584913568983	0.0
9	9.377093729209225E-5	0.0	0.0	3.064715543517451	0.0
10	9.377093729209225E-5	0.0	0.0	3.5359613888788606	0.0
11	9.377093729209225E-5	0.0	0.0	4.1109647763539705	0.0
12	9.377093729209225E-5	0.0	0.0	4.517743102327066	0.0
13	9.377093729209225E-5	0.0	0.0	4.702893818010303	0.0
14	9.377093729209225E-5	0.0	0.0	4.773315791916664	0.0
15	9.377093729209225E-5	0.0	0.0	4.876182510126089	0.0
16	9.377093729209225E-5	0.0	0.0	5.123128273484814	0.0
17	9.377093729209225E-5	0.0	0.0	5.416584421740417	0.0
18	9.377093729209225E-5	0.0	0.0	5.783932068582188	0.0
19	9.377093729209225E-5	0.0	0.0	5.985492698291541	0.0
20	9.377093729209225E-5	0.0	0.0	6.177207379585223	0.0
21	9.377093729209225E-5	0.0	0.0	6.470663527840825	0.0
22	9.377093729209225E-5	0.0	0.0	6.76777674265082	0.0
23	9.377093729209225E-5	0.0	0.0	7.12541909748286	0.0
24	9.377093729209225E-5	0.0	0.0	7.367254344759166	0.0
25	9.377093729209225E-5	0.0	0.0	7.576363534920532	0.0
26	9.377093729209225E-5	0.0	0.0	7.782987795243657	0.0
27	9.377093729209225E-5	0.0	0.0	7.970013929672735	0.0
28	9.377093729209225E-5	0.0	0.0	8.189859892154045	0.0
29	9.377093729209225E-5	0.0	0.0	8.424849861008028	0.0
30	9.377093729209225E-5	0.0	0.0	8.695519671501652	0.0
31	9.377093729209225E-5	0.0	0.0	8.963141926533284	0.0
32	9.377093729209225E-5	0.0	0.0	9.198460093667789	0.0
33	9.377093729209225E-5	0.0	0.0	9.446156024524852	0.0
34	9.377093729209225E-5	0.0	0.0	9.693429986164098	0.0
35	9.377093729209225E-5	0.0	0.0	9.982244473023743	0.0
36	1.4065640593813837E-4	0.0	0.0	10.247803767434947	0.0
37	1.4065640593813837E-4	0.0	0.0	10.518098494179403	0.0
38	1.4065640593813837E-4	0.0	0.0	10.785017467181344	0.0
39	1.4065640593813837E-4	0.0	0.0	11.047060351444095	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCG	35	1.2125747E-7	45.000004	1
CCGATTC	20	7.034645E-4	45.000004	12
CTATCGT	35	1.2125747E-7	45.000004	13
GCGCGTC	35	1.2125747E-7	45.000004	38
TAGAACG	35	1.2125747E-7	45.000004	1
TATGCGT	40	6.8193913E-9	45.000004	20
CCGCTCG	90	0.0	45.000004	19
CCTCGCT	20	7.034645E-4	45.000004	43
CTAAACG	35	1.2125747E-7	45.000004	1
GTCCGTT	40	6.8193913E-9	45.000004	22
TCGTACT	20	7.034645E-4	45.000004	23
ACCGTAA	35	1.2125747E-7	45.000004	25
GCTACCG	35	1.2125747E-7	45.000004	1
TCGGTCA	20	7.034645E-4	45.000004	12
CGTATCA	25	3.8919337E-5	45.0	20
ATCGTCC	25	3.8919337E-5	45.0	37
TGCGCAC	25	3.8919337E-5	45.0	12
GTATCCG	25	3.8919337E-5	45.0	19
CCCGGTC	25	3.8919337E-5	45.0	43
GTCGATC	30	2.16638E-6	44.999996	23
>>END_MODULE