FastQCFastQC Report
Thu 26 May 2016
SRR1548134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548134_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1475582
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122540.8304519843695571No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC60600.4106854109090514No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA302.1659216E-645.00000440
TCGTTGA2300.045.00000424
CGACGGT2050.045.00000428
GCGAACG600.045.0000041
TAGCGAT302.1659216E-645.00000412
TATTCGA302.1659216E-645.00000418
ATGCGTC302.1659216E-645.00000437
TCGAAGT302.1659216E-645.00000420
GCATCGA302.1659216E-645.0000049
AATCCGT207.0339383E-445.042
CGAACTA453.8562575E-1045.034
ACACGAT253.8913455E-545.015
ACACGAG453.8562575E-1045.01
GATCGTC406.8175723E-945.09
TTTACCG253.8913455E-545.01
ACGCATC253.8913455E-545.010
TCTTCGC253.8913455E-545.01
CGCATCG551.8189894E-1245.021
CCAATCG850.045.024
CCTAACG207.0339383E-445.011
Produced by FastQC (version 0.11.5)