##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548134_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1475582
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.169072948843237	31.0	31.0	33.0	30.0	34.0
2	31.54268010859444	31.0	31.0	34.0	30.0	34.0
3	31.579132843854154	31.0	31.0	34.0	30.0	34.0
4	35.4134693971599	37.0	35.0	37.0	33.0	37.0
5	35.27131735139084	37.0	35.0	37.0	33.0	37.0
6	35.324068062635625	37.0	35.0	37.0	33.0	37.0
7	35.7891455710357	37.0	35.0	37.0	35.0	37.0
8	35.81099051086283	37.0	35.0	37.0	35.0	37.0
9	37.59353597427998	39.0	37.0	39.0	35.0	39.0
10	36.868704687370816	39.0	37.0	39.0	32.0	39.0
11	36.45598346957336	38.0	35.0	39.0	32.0	39.0
12	35.2971003983513	37.0	35.0	39.0	30.0	39.0
13	34.70898398055818	37.0	33.0	39.0	27.0	39.0
14	35.54903353388697	37.0	33.0	40.0	27.0	41.0
15	36.0191517652018	37.0	34.0	40.0	30.0	41.0
16	36.28922553948205	37.0	35.0	40.0	31.0	41.0
17	36.21395964439794	37.0	35.0	40.0	31.0	41.0
18	36.16478650457921	36.0	35.0	40.0	31.0	41.0
19	36.030516094666375	36.0	35.0	40.0	31.0	41.0
20	35.81189523862449	36.0	34.0	40.0	31.0	41.0
21	35.61818658671629	35.0	34.0	40.0	30.0	41.0
22	35.505071897054854	35.0	34.0	40.0	30.0	41.0
23	35.531250720054864	35.0	34.0	40.0	30.0	41.0
24	35.349464821338294	35.0	34.0	40.0	30.0	41.0
25	35.25546462345027	35.0	34.0	40.0	30.0	41.0
26	35.169582578264034	35.0	34.0	40.0	30.0	41.0
27	35.10236571061452	35.0	34.0	40.0	29.0	41.0
28	35.21065111935494	35.0	34.0	40.0	29.0	41.0
29	35.323806470938244	36.0	34.0	40.0	30.0	41.0
30	35.275899949985835	36.0	34.0	40.0	30.0	41.0
31	35.018397486551066	35.0	34.0	40.0	29.0	41.0
32	34.74175139029888	35.0	34.0	40.0	28.0	41.0
33	34.56474123430619	35.0	33.0	40.0	27.0	41.0
34	34.422246950694706	35.0	34.0	40.0	26.0	41.0
35	34.23604584496152	35.0	33.0	40.0	25.0	41.0
36	33.942472190633936	35.0	33.0	40.0	23.0	41.0
37	33.82148060900716	35.0	33.0	40.0	23.0	41.0
38	33.79906843536991	35.0	33.0	39.0	23.0	41.0
39	33.835914235874384	35.0	33.0	39.0	23.0	41.0
40	33.67617387579951	35.0	33.0	39.0	23.0	41.0
41	33.684458742380976	35.0	33.0	39.0	23.0	41.0
42	33.615030543880316	35.0	33.0	39.0	23.0	41.0
43	33.485587381792406	35.0	33.0	39.0	23.0	41.0
44	33.34091429686727	35.0	33.0	39.0	23.0	41.0
45	33.23893894070272	35.0	32.0	39.0	23.0	41.0
46	33.15872245663067	35.0	32.0	39.0	23.0	41.0
47	33.10639395167466	35.0	32.0	39.0	23.0	41.0
48	33.06315203085969	35.0	32.0	39.0	23.0	40.0
49	33.02516701884409	35.0	32.0	38.0	22.0	40.0
50	32.817512005432434	35.0	32.0	38.0	21.0	40.0
51	32.38903090441602	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	2.0
13	4.0
14	15.0
15	51.0
16	154.0
17	352.0
18	729.0
19	1585.0
20	2836.0
21	4544.0
22	7056.0
23	10734.0
24	15836.0
25	22150.0
26	29692.0
27	33992.0
28	36364.0
29	40729.0
30	48358.0
31	59080.0
32	74932.0
33	98102.0
34	171634.0
35	246999.0
36	101848.0
37	112690.0
38	149767.0
39	205305.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.43802377638112	23.951566229460646	30.779583920107456	14.830826074050782
2	29.830737973220057	25.646829522181758	31.379686117071092	13.14274638752709
3	27.0485137389857	24.52069759593164	34.80545303480254	13.62533563028012
4	24.296243787197184	27.20594314650084	34.28843669819773	14.209376368104246
5	22.427760707300575	31.33590678118871	33.09053647984321	13.145796031667507
6	20.342414044085654	39.16705408442228	29.911248578526983	10.57928329296508
7	86.44738144000131	3.975448331573576	8.253353592006409	1.323816636418715
8	88.09988194488683	2.746848362205557	7.480167147606842	1.6731025453007693
9	84.84950345016406	4.245104643455938	8.8563021235011	2.0490897828788914
10	55.749934602075655	21.620892637616887	13.965133757391998	8.664039002915459
11	48.86424475224013	19.0479417612847	20.996935446488234	11.090878039986935
12	44.23380062917548	20.257362857503004	23.42770513600735	12.081131377314172
13	20.394732383561198	44.38350427153489	23.05774941684027	12.164013928063639
14	13.384210433578072	46.77801708071798	27.097036965753173	12.740735519950771
15	11.428914150484351	25.216490849034486	51.532480065492805	11.822114934988363
16	12.861908047129877	19.83495325912081	50.766544997160445	16.536593696588874
17	13.515141822006505	21.15341607582635	28.54385591583524	36.7875861863319
18	20.029317245669844	24.81054932901052	36.35928060927824	18.8008528160414
19	27.60700523590014	27.5317806804366	25.83590745888741	19.02530662477585
20	30.754712377895636	24.333246136100875	26.529667615896642	18.382373870106846
21	20.06299887095397	31.02321660199162	29.174454554203024	19.73932997285139
22	21.51564602983772	24.561359517803822	26.749174224136645	27.173820228221814
23	16.352056341158946	32.855578341291775	25.388151929204884	25.40421338834439
24	18.57253612472909	24.026316395835675	41.52571663248806	15.87543084694717
25	13.849043970446914	27.273170857329514	38.28753671432696	20.590248457896614
26	13.998815382676124	38.91400139063773	28.314929295694853	18.772253930991297
27	14.932413108861454	38.99092019284594	30.84938688598804	15.22727981230457
28	12.492155637572159	30.77768636375342	41.49976077235965	15.230397226314768
29	12.364206123414355	26.025798634030505	41.402511009215345	20.207484233339795
30	15.881462365358212	33.858030255180665	33.31783662310871	16.94267075635241
31	28.450469035268792	28.904798242320656	25.631987920698407	17.012744801712138
32	28.408722795480017	28.90188413791982	27.551637252284184	15.137755814315978
33	26.894947214048422	30.59497879480774	24.49758807033428	18.012485920809553
34	17.220730532088353	31.166346566981705	27.625370870612407	23.98755203031753
35	17.66069252674538	28.5955643264827	31.843909725111853	21.899833421660063
36	30.16999394137364	25.07424189235163	27.770466161826317	16.985298004448417
37	17.681633416509555	34.22127675723884	31.72280496780254	16.374284858449073
38	17.91428737948823	35.22847256201282	24.248330489257796	22.608909569241153
39	17.976364580213094	33.13980517517834	29.950690642742998	18.93313960186557
40	23.395785527337686	28.440439094540327	25.489129035187474	22.67464634293452
41	15.732233112087298	25.6554362956447	28.997371884449663	29.61495870781834
42	21.71929448854757	25.5732314435931	25.660519035878725	27.046955031980602
43	21.71909117893821	27.370556160213393	25.828994932169135	25.08135772867926
44	17.094543034545012	33.568517371450724	28.83980693719495	20.497132656809313
45	15.407005506979617	42.15211353892905	21.8803834690312	20.560497485060132
46	22.445245333705614	35.49324944327052	24.562443835720412	17.49906138730345
47	19.841255857011	31.041717776443466	26.069781279522246	23.047245087023292
48	22.19266702900957	26.85001579037966	31.14838755148816	19.80892962912261
49	20.320117756925743	24.951646197906996	31.83503187216976	22.893204172997503
50	18.383525957893223	34.22812151408732	27.734073741750713	19.65427878626874
51	16.94578817036261	36.79409209383145	23.579848493679105	22.680271242126835
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	816.0
1	1205.5
2	1595.0
3	9110.5
4	16626.0
5	12061.0
6	7496.0
7	8191.0
8	8886.0
9	10464.5
10	12043.0
11	12984.0
12	13925.0
13	13875.0
14	13825.0
15	12756.5
16	11688.0
17	10603.5
18	9519.0
19	9032.5
20	8546.0
21	8224.0
22	7902.0
23	8082.0
24	8262.0
25	8654.5
26	10869.5
27	12692.0
28	14561.5
29	16431.0
30	17628.5
31	18826.0
32	24161.0
33	29496.0
34	33609.5
35	37723.0
36	42438.0
37	47153.0
38	57465.0
39	67777.0
40	94132.0
41	120487.0
42	145004.0
43	169521.0
44	170171.0
45	170821.0
46	160793.5
47	150766.0
48	138055.0
49	125344.0
50	120372.0
51	115400.0
52	101640.5
53	87881.0
54	71889.5
55	55898.0
56	47894.0
57	39890.0
58	32990.0
59	26090.0
60	22912.5
61	19735.0
62	16372.5
63	13010.0
64	10505.0
65	8000.0
66	6591.5
67	5183.0
68	4049.5
69	2916.0
70	2249.5
71	1583.0
72	1439.5
73	1296.0
74	1018.0
75	509.5
76	279.0
77	207.5
78	136.0
79	149.5
80	163.0
81	142.0
82	121.0
83	68.5
84	16.0
85	13.0
86	10.0
87	8.0
88	6.0
89	5.5
90	5.0
91	3.0
92	1.0
93	4.0
94	7.0
95	4.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1475582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.348429971880954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.3768417998303	19.57049903924122
2	8.798482592590075	4.28458474528986
3	3.12190027069565	2.2804011036068763
4	1.5154648092407408	1.475967551305924
5	0.9286543038276069	1.1305637142416174
6	0.592812220514653	0.8660428102605765
7	0.4282260419890575	0.7298642256854423
8	0.3035495574845215	0.5912764114725859
9	0.2505790377912853	0.5491085538674179
>10	1.9665823082071425	10.271127210137031
>50	0.6761794792724978	12.223580389012918
>100	1.0314878663982088	43.42207556991738
>500	0.008119747048168322	1.1886727996351436
>1k	5.599825550460912E-4	0.16770676113228108
>5k	2.799912775230456E-4	0.41313128961854606
>10k+	2.799912775230456E-4	0.8353978255751922
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12254	0.8304519843695571	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6060	0.4106854109090514	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06316151864145808	0.0
2	0.0	0.0	0.0	0.21855783006298532	0.0
3	0.0	0.0	0.0	0.319196086696639	0.0
4	0.0	0.0	0.0	0.484961188195573	0.0
5	0.0	0.0	0.0	0.831129683067427	0.0
6	0.0	0.0	0.0	1.2163336229365769	0.0
7	0.0	0.0	0.0	1.4752145255228106	0.0
8	0.0	0.0	0.0	2.132243413107506	0.0
9	0.0	0.0	0.0	2.349310306035178	0.0
10	0.0	0.0	0.0	2.7723976031152455	0.0
11	0.0	0.0	0.0	3.318283904249306	0.0
12	0.0	0.0	0.0	3.719007144299673	0.0
13	0.0	0.0	0.0	3.8882962790275295	0.0
14	0.0	0.0	0.0	3.9517288771481356	0.0
15	0.0	0.0	0.0	4.042337193053317	0.0
16	0.0	0.0	0.0	4.282852460927281	0.0
17	0.0	0.0	0.0	4.58192089629719	0.0
18	0.0	0.0	0.0	4.959602380619986	0.0
19	0.0	0.0	0.0	5.158778027923897	0.0
20	0.0	0.0	0.0	5.357411516269512	0.0
21	0.0	0.0	0.0	5.667187591065763	0.0
22	0.0	0.0	0.0	5.970864377581185	0.0
23	0.0	0.0	0.0	6.339532469222314	0.0
24	0.0	0.0	0.0	6.584385008762644	0.0
25	0.0	0.0	0.0	6.805585863747321	0.0
26	0.0	0.0	0.0	7.013436054383965	0.0
27	0.0	0.0	0.0	7.215119186869995	0.0
28	0.0	0.0	0.0	7.448247538937179	0.0
29	0.0	0.0	0.0	7.695946413008562	0.0
30	0.0	0.0	0.0	7.978546770020236	0.0
31	0.0	0.0	0.0	8.258097482891497	0.0
32	0.0	0.0	0.0	8.513793201597743	0.0
33	0.0	0.0	0.0	8.765829347335492	0.0
34	0.0	0.0	0.0	9.016916714896224	0.0
35	0.0	0.0	0.0	9.331436680577562	0.0
36	0.0	0.0	0.0	9.614240347198598	0.0
37	0.0	0.0	0.0	9.90266891301195	0.0
38	0.0	0.0	0.0	10.176120337602383	0.0
39	0.0	0.0	0.0	10.444624561698367	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	30	2.1659216E-6	45.000004	40
TCGTTGA	230	0.0	45.000004	24
CGACGGT	205	0.0	45.000004	28
GCGAACG	60	0.0	45.000004	1
TAGCGAT	30	2.1659216E-6	45.000004	12
TATTCGA	30	2.1659216E-6	45.000004	18
ATGCGTC	30	2.1659216E-6	45.000004	37
TCGAAGT	30	2.1659216E-6	45.000004	20
GCATCGA	30	2.1659216E-6	45.000004	9
AATCCGT	20	7.0339383E-4	45.0	42
CGAACTA	45	3.8562575E-10	45.0	34
ACACGAT	25	3.8913455E-5	45.0	15
ACACGAG	45	3.8562575E-10	45.0	1
GATCGTC	40	6.8175723E-9	45.0	9
TTTACCG	25	3.8913455E-5	45.0	1
ACGCATC	25	3.8913455E-5	45.0	10
TCTTCGC	25	3.8913455E-5	45.0	1
CGCATCG	55	1.8189894E-12	45.0	21
CCAATCG	85	0.0	45.0	24
CCTAACG	20	7.0339383E-4	45.0	11
>>END_MODULE