FastQCFastQC Report
Thu 26 May 2016
SRR1548133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548133_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2283616
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT511012.23772297969536No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT37660.16491389095189382No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT28000.12261255832854559No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT262800.043.92981
CGACGGT1500.042.00000428
CTACGCG403.4602272E-739.3751
GTTTTTT303400.038.666782
ATAGCGG2800.038.5714262
GGGCGAT26600.038.4022567
CAACGCG659.094947E-1238.076921
TAGGGAT32400.037.777785
GTAGGGA25850.037.6015474
TAAGGGA28150.037.406754
AGGGCGA13500.037.3333326
GCGTAAG1450.037.241381
GGCGATT8850.037.1186458
AGGGATC26600.036.87976
AGGGATT40300.036.8486336
AAGGGAT33950.036.7820325
TACGGGA9300.036.7741934
CTCGTCC3800.036.71052637
ACGGGAC5850.036.538465
GCGATAT1850.036.4864859
Produced by FastQC (version 0.11.5)