##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548133_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2283616
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.108698222468227	31.0	31.0	33.0	30.0	34.0
2	31.48992387511736	31.0	31.0	34.0	30.0	34.0
3	31.37870815408545	31.0	31.0	34.0	30.0	34.0
4	35.3091110764682	37.0	35.0	37.0	33.0	37.0
5	35.20977913975029	37.0	35.0	37.0	32.0	37.0
6	35.27182065636254	37.0	35.0	37.0	32.0	37.0
7	35.74074494135616	37.0	35.0	37.0	35.0	37.0
8	35.7490685824587	37.0	35.0	37.0	35.0	37.0
9	37.472346926978965	39.0	37.0	39.0	35.0	39.0
10	36.81781394069756	39.0	37.0	39.0	32.0	39.0
11	36.46243326373611	39.0	35.0	39.0	32.0	39.0
12	36.0839637662374	38.0	35.0	39.0	31.0	39.0
13	35.93603083881003	38.0	35.0	39.0	31.0	39.0
14	36.92215678993316	39.0	35.0	41.0	31.0	41.0
15	37.103344432689205	39.0	35.0	41.0	31.0	41.0
16	37.13975729719883	39.0	35.0	41.0	32.0	41.0
17	36.966374381682385	39.0	35.0	41.0	31.0	41.0
18	36.770989518377874	39.0	35.0	40.0	31.0	41.0
19	36.588569181508625	38.0	35.0	40.0	31.0	41.0
20	36.35466076608326	38.0	35.0	40.0	31.0	41.0
21	36.232057403696594	38.0	35.0	40.0	30.0	41.0
22	36.18296508694982	38.0	35.0	40.0	30.0	41.0
23	36.10789554811317	37.0	35.0	40.0	30.0	41.0
24	35.939999106679934	37.0	34.0	40.0	30.0	41.0
25	35.823821518153665	37.0	34.0	40.0	30.0	41.0
26	35.751430187912504	37.0	34.0	40.0	30.0	41.0
27	35.69531479898547	37.0	34.0	40.0	30.0	41.0
28	35.643965973263455	37.0	34.0	40.0	29.0	41.0
29	35.59937353740734	37.0	34.0	40.0	29.0	41.0
30	35.42959411739977	37.0	34.0	40.0	29.0	41.0
31	35.21127369925592	37.0	34.0	40.0	27.0	41.0
32	34.89144059246388	37.0	34.0	40.0	25.0	41.0
33	34.56545277314575	37.0	33.0	40.0	23.0	41.0
34	34.18130631419643	37.0	33.0	40.0	22.0	41.0
35	33.86709367949778	37.0	33.0	40.0	20.0	41.0
36	33.693700692235474	37.0	33.0	40.0	18.0	41.0
37	33.6256240103415	37.0	33.0	40.0	18.0	41.0
38	33.520400978097896	37.0	33.0	40.0	18.0	41.0
39	33.46674834998529	37.0	33.0	40.0	18.0	41.0
40	33.34936653097544	36.0	32.0	40.0	18.0	41.0
41	33.248201098608526	36.0	32.0	40.0	17.0	41.0
42	33.20113626809411	36.0	32.0	40.0	17.0	41.0
43	33.090097021565796	36.0	32.0	40.0	16.0	41.0
44	32.939344443198856	36.0	31.0	40.0	15.0	41.0
45	32.874008151983524	36.0	31.0	40.0	15.0	41.0
46	32.765457940389275	36.0	31.0	40.0	15.0	41.0
47	32.73851908552051	35.0	31.0	40.0	15.0	41.0
48	32.61099195311296	35.0	31.0	40.0	15.0	41.0
49	32.50237167719967	35.0	31.0	40.0	15.0	41.0
50	32.39188199767386	35.0	31.0	39.0	15.0	41.0
51	32.08160128497961	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	2.0
12	4.0
13	7.0
14	47.0
15	111.0
16	338.0
17	810.0
18	1911.0
19	3584.0
20	6062.0
21	9379.0
22	14120.0
23	20740.0
24	29373.0
25	43212.0
26	59382.0
27	68065.0
28	68643.0
29	68377.0
30	73291.0
31	84292.0
32	99540.0
33	122539.0
34	178829.0
35	219903.0
36	181653.0
37	213346.0
38	298846.0
39	417157.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.805105586928804	22.435733503356083	26.86336056499867	21.89580034471645
2	37.237871866373325	23.20849915222174	27.29513193111276	12.258497050292167
3	25.887627341899865	23.308603548057118	38.19350538794614	12.610263722096885
4	23.688133206283368	25.411277552793464	37.568312711068764	13.332276529854406
5	21.918133346412006	28.920273811358825	35.93104094558805	13.230551896641115
6	20.491448649860573	36.78065839440606	32.89826310553088	9.829629850202485
7	80.29778211398063	3.1827154828132227	14.778141333744376	1.7413610694617658
8	81.26059722825553	2.4803644745876716	14.424053781371299	1.834984515785491
9	76.60758201028544	4.949737609125177	15.699837450779816	2.7428429298095653
10	38.71022974090215	31.92949252413716	20.45681060213276	8.903467132827936
11	27.71621848857251	22.79126613230946	34.76587131987164	14.726644059246388
12	27.663976780684667	21.004056724072697	35.05422978294074	16.277736712301895
13	22.1318295194989	24.72079368860614	35.66032993287838	17.487046859016576
14	16.304010831943724	28.52703782071942	37.28223133836862	17.886720008968233
15	16.60738933340807	25.943722587335174	41.941202023457535	15.507686055799224
16	21.33738772192873	24.46554061628575	39.58362526799602	14.613446393789499
17	20.453964239171558	24.212258102938495	35.68432696215125	19.649450695738686
18	21.047803133276346	25.306969297815396	36.982881535249355	16.6623460336589
19	22.36943514145986	27.151981769264182	33.626537911803034	16.85204517747292
20	23.90152284797444	27.094572817846785	33.37461289463728	15.629291439541499
21	22.26652817286269	26.217936815997085	35.478600605355716	16.03693440578451
22	20.783573070078333	23.355896963412413	37.00420736235864	18.85632260415061
23	18.98296386082424	25.88653258691479	36.356550313187505	18.773953239073467
24	19.67931561173157	25.04812542914395	38.30753506719169	16.965023891932795
25	19.397175356977705	26.367130025363284	35.58658723708364	18.64910738057537
26	17.730958269691577	29.3474472065356	34.12806706556619	18.793527458206633
27	17.465896192704903	28.19015981671174	36.22474181298432	18.119202177599036
28	16.666900214396815	27.712146000028028	37.849971273629194	17.770982511945967
29	18.63036517523086	25.907332931631238	37.006002760534166	18.45629913260373
30	19.42349326681894	25.98322134719673	36.79348892283116	17.799796463153175
31	22.516745372251727	25.346643218474558	34.15110071045219	17.985510698821518
32	23.36360403850735	25.946919271891595	33.537862758011855	17.1516139315892
33	22.37990099911719	26.38915649566302	33.26434917254039	17.966593332679402
34	19.097475232263218	26.946693314462678	35.01267288370724	18.94315856956686
35	18.460415411347615	28.452901013130056	34.198131384611074	18.888552190911255
36	20.58165646063086	29.01341556548912	32.02902764738029	18.37590032649973
37	19.80184058966131	30.205122052043777	31.80788714039488	18.18515021790003
38	19.286867844681417	30.58307526309152	30.542175216849067	19.587881675377997
39	19.646122640584057	29.599766335495985	30.79694659697602	19.957164426943937
40	20.92702976332273	28.128941117946276	30.643856059862955	20.30017305886804
41	18.168378571528663	27.95351757913765	31.622698387119375	22.255405462214313
42	19.583283704440678	28.600955677311774	30.165929823578047	21.649830794669505
43	19.81274434931267	28.205573966901614	30.405374633913933	21.576307049871783
44	19.437462340428514	29.68165400837969	30.44264009360593	20.438243557585864
45	19.33652593080448	29.93467378053053	29.79007854210165	20.938721746563346
46	20.15527128904334	30.053564171909812	29.786487745750602	20.004676793296248
47	19.080922536888863	28.891897762145653	31.48186910583916	20.545310595126328
48	19.810686209940727	27.166826646861818	33.02144493645166	20.001042206745794
49	19.828070919103737	26.562083993105674	32.51431063716492	21.095534450625674
50	18.242165057522804	28.73692424645825	32.48457709177025	20.5363336042487
51	17.679986477586425	28.940198352087215	32.221222832560294	21.15859233776607
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1606.0
1	2732.5
2	3859.0
3	41259.0
4	78659.0
5	54145.0
6	29631.0
7	28770.5
8	27910.0
9	28803.5
10	29697.0
11	29889.0
12	30081.0
13	29472.5
14	28864.0
15	26852.5
16	24841.0
17	23347.5
18	21854.0
19	20583.5
20	19313.0
21	19299.0
22	19285.0
23	19476.5
24	19668.0
25	20919.5
26	25146.0
27	28121.0
28	33192.0
29	38263.0
30	43189.0
31	48115.0
32	54507.5
33	60900.0
34	68855.5
35	76811.0
36	81438.5
37	86066.0
38	94071.0
39	102076.0
40	114256.0
41	126436.0
42	137900.5
43	149365.0
44	156680.0
45	163995.0
46	168570.5
47	173146.0
48	167576.0
49	162006.0
50	152632.5
51	143259.0
52	132041.5
53	120824.0
54	111451.0
55	102078.0
56	95585.0
57	89092.0
58	83303.0
59	77514.0
60	71575.0
61	65636.0
62	56615.5
63	47595.0
64	37709.5
65	27824.0
66	21975.5
67	16127.0
68	12475.5
69	8824.0
70	7093.0
71	5362.0
72	4259.5
73	3157.0
74	2388.0
75	1252.5
76	886.0
77	720.5
78	555.0
79	404.5
80	254.0
81	180.0
82	106.0
83	80.5
84	55.0
85	42.0
86	29.0
87	28.5
88	28.0
89	34.0
90	40.0
91	24.5
92	9.0
93	5.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2283616.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.690713132624843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.80154473959448	21.543802566591882
2	6.102444211461525	3.3796206413485623
3	2.5793807122138013	2.1427467408521377
4	1.6680150821574444	1.8475410856365382
5	1.1853990578703728	1.6412272629586124
6	0.8911442540298109	1.4805851938875874
7	0.7216581637934318	1.39882604353945
8	0.6198025154514429	1.3730218923396147
9	0.5006092439719844	1.2476004269731582
>10	7.169535389001378	46.546253818463434
>50	0.7178397956607182	12.380562055120247
>100	0.03926179100999863	1.6989910975731746
>500	0.0017626419819442316	0.34632071744744736
>1k	0.001442161621590735	0.7054648413449026
>5k	0.0	0.0
>10k+	1.6024018017674833E-4	2.2674356159233833
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51101	2.23772297969536	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT	3766	0.16491389095189382	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT	2800	0.12261255832854559	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.75160797612208E-4	0.0	0.0	0.03389361433796225	0.0
2	1.75160797612208E-4	0.0	0.0	0.11858385998346482	0.0
3	1.75160797612208E-4	0.0	0.0	0.2436048792791783	0.0
4	1.75160797612208E-4	0.0	0.0	0.35868552331039893	0.0
5	1.75160797612208E-4	0.0	0.0	0.683740173479254	0.0
6	1.75160797612208E-4	0.0	0.0	1.2602819388198365	0.0
7	1.75160797612208E-4	0.0	0.0	1.701862309600213	0.0
8	1.75160797612208E-4	0.0	0.0	2.2950443507139555	0.0
9	1.75160797612208E-4	0.0	0.0	2.6915646063085914	0.0
10	1.75160797612208E-4	0.0	0.0	3.0967991115844344	0.0
11	1.75160797612208E-4	0.0	0.0	3.370838179448734	0.0
12	1.75160797612208E-4	0.0	0.0	3.5879937782884688	0.0
13	1.75160797612208E-4	0.0	0.0	3.7081102952510405	0.0
14	1.75160797612208E-4	0.0	0.0	3.789209744545493	0.0
15	1.75160797612208E-4	0.0	0.0	3.853581337667979	0.0
16	1.75160797612208E-4	0.0	0.0	3.942519232655578	0.0
17	1.75160797612208E-4	0.0	0.0	4.036799531970349	0.0
18	1.75160797612208E-4	0.0	0.0	4.168345290977117	0.0
19	1.75160797612208E-4	0.0	0.0	4.255925689783221	0.0
20	1.75160797612208E-4	0.0	0.0	4.346483822148732	0.0
21	1.75160797612208E-4	0.0	0.0	4.4482522455614255	0.0
22	1.75160797612208E-4	0.0	0.0	4.564077322982498	0.0
23	1.75160797612208E-4	0.0	0.0	4.690017936465676	0.0
24	1.75160797612208E-4	0.0	0.0	4.7941948208455365	0.0
25	1.75160797612208E-4	0.0	0.0	4.889613665344786	0.0
26	1.75160797612208E-4	0.0	0.0	4.9817482448888075	0.0
27	1.75160797612208E-4	0.0	0.0	5.083823199697322	0.0
28	1.75160797612208E-4	0.0	0.0	5.188262825273601	0.0
29	1.75160797612208E-4	0.0	0.0	5.3087734540308	0.0
30	2.1895099701526002E-4	0.0	0.0	5.461907781343273	0.0
31	2.1895099701526002E-4	0.0	0.0	5.588154926222272	0.0
32	2.1895099701526002E-4	0.0	0.0	5.717336014461275	0.0
33	2.1895099701526002E-4	0.0	0.0	5.842926306349229	0.0
34	2.1895099701526002E-4	0.0	0.0	5.9810843854658575	0.0
35	2.1895099701526002E-4	0.0	0.0	6.138116040525202	0.0
36	2.1895099701526002E-4	0.0	0.0	6.280915880778554	0.0
37	2.1895099701526002E-4	0.0	0.0	6.442326555778204	0.0
38	2.1895099701526002E-4	0.0	0.0	6.641440592463882	0.0
39	2.1895099701526002E-4	0.0	0.0	6.901641957316817	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	26280	0.0	43.9298	1
CGACGGT	150	0.0	42.000004	28
CTACGCG	40	3.4602272E-7	39.375	1
GTTTTTT	30340	0.0	38.66678	2
ATAGCGG	280	0.0	38.571426	2
GGGCGAT	2660	0.0	38.402256	7
CAACGCG	65	9.094947E-12	38.07692	1
TAGGGAT	3240	0.0	37.77778	5
GTAGGGA	2585	0.0	37.601547	4
TAAGGGA	2815	0.0	37.40675	4
AGGGCGA	1350	0.0	37.333332	6
GCGTAAG	145	0.0	37.24138	1
GGCGATT	885	0.0	37.118645	8
AGGGATC	2660	0.0	36.8797	6
AGGGATT	4030	0.0	36.848633	6
AAGGGAT	3395	0.0	36.782032	5
TACGGGA	930	0.0	36.774193	4
CTCGTCC	380	0.0	36.710526	37
ACGGGAC	585	0.0	36.53846	5
GCGATAT	185	0.0	36.486485	9
>>END_MODULE