FastQCFastQC Report
Thu 26 May 2016
SRR1548132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548132_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1427566
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143261.0035262817971287No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC50680.35500985593660817No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAAT302.1658725E-645.0000046
TACGGAG351.2122109E-745.0000041
TCCTTAC351.2122109E-745.00000413
GTGCGTA351.2122109E-745.00000419
ATCGCCG351.2122109E-745.00000422
CGGACCG302.1658725E-645.00000416
GTGCAAC207.033862E-445.043
CTAGACG502.1827873E-1145.01
GTCGCGA207.033862E-445.01
TAGACGG1750.045.02
CGCATCA207.033862E-445.020
GCTGCGC207.033862E-445.023
TCCTCGA207.033862E-445.027
TTGTACG406.8175723E-945.01
GCGTTCC207.033862E-445.010
ATGTACG406.8175723E-945.01
AAACGTC207.033862E-445.032
TGTTGCG1000.045.01
GCCGAAT207.033862E-445.08
GTTCGCG253.8912818E-545.01