##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548132_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1427566
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.06026201240433	31.0	31.0	33.0	30.0	34.0
2	31.4148964040892	31.0	31.0	34.0	30.0	34.0
3	31.453077475927557	31.0	31.0	34.0	30.0	34.0
4	35.28797477664781	37.0	35.0	37.0	33.0	37.0
5	35.12687749638195	37.0	35.0	37.0	32.0	37.0
6	35.18816152808346	37.0	35.0	37.0	32.0	37.0
7	35.71240419006897	37.0	35.0	37.0	35.0	37.0
8	35.734369549288786	37.0	35.0	37.0	35.0	37.0
9	37.497474722709846	39.0	37.0	39.0	35.0	39.0
10	36.70409073906215	39.0	37.0	39.0	32.0	39.0
11	36.28744520393453	38.0	35.0	39.0	32.0	39.0
12	35.07690012230608	35.0	34.0	39.0	30.0	39.0
13	34.458301052280596	35.0	33.0	39.0	26.0	39.0
14	35.24830095421157	36.0	33.0	40.0	26.0	41.0
15	35.76713651067622	36.0	33.0	40.0	30.0	41.0
16	36.069188394792256	36.0	34.0	40.0	31.0	41.0
17	35.98574286582897	36.0	34.0	40.0	31.0	41.0
18	35.924914855075	36.0	34.0	40.0	30.0	41.0
19	35.77982594149763	36.0	34.0	40.0	30.0	41.0
20	35.57086187258592	36.0	34.0	40.0	30.0	41.0
21	35.342422697094214	35.0	34.0	40.0	29.0	41.0
22	35.22288006298833	35.0	34.0	40.0	29.0	41.0
23	35.26432963519725	35.0	34.0	40.0	29.0	41.0
24	35.092484690725335	35.0	34.0	40.0	29.0	41.0
25	34.99572629216443	35.0	34.0	39.0	29.0	41.0
26	34.87164306238731	35.0	34.0	39.0	29.0	41.0
27	34.78367234859894	35.0	34.0	39.0	29.0	41.0
28	34.824237198140054	35.0	34.0	39.0	28.0	41.0
29	34.93910754388939	35.0	34.0	39.0	29.0	41.0
30	34.865591503300024	36.0	34.0	39.0	29.0	41.0
31	34.619275045777215	35.0	34.0	39.0	27.0	41.0
32	34.33909675629708	35.0	33.0	39.0	26.0	41.0
33	34.14515335893402	35.0	33.0	39.0	25.0	41.0
34	34.01998786746112	35.0	33.0	39.0	24.0	41.0
35	33.85975009211483	35.0	33.0	39.0	23.0	41.0
36	33.569578569397144	35.0	33.0	39.0	23.0	41.0
37	33.443290187634055	35.0	33.0	39.0	23.0	41.0
38	33.42441190109599	35.0	33.0	39.0	23.0	41.0
39	33.468590594060096	35.0	33.0	39.0	23.0	41.0
40	33.33378631881118	35.0	32.0	39.0	23.0	41.0
41	33.3357855258531	35.0	33.0	39.0	23.0	41.0
42	33.26663005423217	35.0	32.0	39.0	22.0	41.0
43	33.12821823999731	35.0	32.0	39.0	22.0	41.0
44	33.01044715270607	35.0	32.0	39.0	22.0	41.0
45	32.920657258578586	35.0	32.0	39.0	22.0	41.0
46	32.81241077470324	35.0	32.0	38.0	21.0	40.0
47	32.78061399613048	35.0	32.0	38.0	21.0	40.0
48	32.72121569160375	35.0	32.0	38.0	20.0	40.0
49	32.6775644698739	35.0	32.0	38.0	20.0	40.0
50	32.45422208150096	35.0	31.0	38.0	20.0	40.0
51	32.05204663041849	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	3.0
13	7.0
14	20.0
15	55.0
16	201.0
17	500.0
18	1092.0
19	2135.0
20	3691.0
21	5694.0
22	8681.0
23	12554.0
24	17748.0
25	24526.0
26	31301.0
27	35856.0
28	38638.0
29	43359.0
30	50714.0
31	60957.0
32	74987.0
33	96562.0
34	164476.0
35	233177.0
36	97173.0
37	104227.0
38	136799.0
39	182388.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.79647455879448	23.339026006503378	30.647829942713685	15.21666949198846
2	30.78876913571772	25.431818914151783	30.479081177332606	13.300330772797894
3	26.551837183009404	24.737280097732782	34.64295170941308	14.067931009844727
4	24.36896087466359	27.41862722984437	33.98904148739883	14.223370408093217
5	22.11974787855693	32.04671447764937	32.81214318637457	13.021394457419131
6	20.370686889432783	39.36343398483853	29.251186985400324	11.014692140328364
7	86.25632720308553	4.093190787676367	8.280387736889223	1.3700942723488791
8	87.97162442927332	2.7644956520399058	7.647982650189203	1.6158972684975685
9	84.81212077059834	4.247649495715084	8.92351036659601	2.0167193670905585
10	55.53067248729656	20.540416345023626	14.808141970318712	9.120769197361103
11	50.0031522185314	18.497708687374175	19.772535910774003	11.726603183320421
12	44.37756292878928	21.18557040445065	22.09460017960641	12.34226648715366
13	21.03167209081752	44.98685174625901	22.63061742854621	11.350858734377255
14	13.304043385734879	47.60655549375651	26.95749268335054	12.131908437158073
15	11.367320320041244	25.931550625330107	51.28645540731567	11.414673647312979
16	12.966195608469242	20.436813429291536	50.494828260129474	16.102162702109744
17	14.12404049970369	20.35625673348903	29.31269027141302	36.20701249539426
18	20.324454350972214	24.50772853934599	36.14950201952134	19.018315090160453
19	27.79570261550079	27.22199884278555	25.93309170994546	19.0492068317682
20	30.557816591316968	25.367723804013266	26.20082013721257	17.8736394674572
21	20.013575554475242	30.520200116842233	29.66279667630078	19.803427652381746
22	20.776132241871828	25.712856708551477	27.0525495843975	26.458461465179195
23	15.768097587081789	32.72339072239042	25.526875815198736	25.981635875329058
24	17.616278336693362	26.05967079630644	39.727480200565154	16.59657066643504
25	14.217416217533902	26.98733368544782	37.897021924030135	20.898228172988148
26	13.620596175588378	38.90412072016285	28.190850720737252	19.284432383511515
27	15.32818797869941	38.80598164988519	30.40223709446709	15.463593276948318
28	12.579033123512328	31.29410479095187	40.901926776065	15.224935309470805
29	12.559419319316936	26.460282746997336	40.790478338654744	20.189819595030983
30	16.018733984978628	34.38208811361436	32.67967995875497	16.91949794265204
31	29.025067842747728	28.947943562679413	24.892859594582667	17.134128999990192
32	28.489050593807924	29.47646553644455	26.769200163074775	15.265283706672756
33	26.623777814826077	31.598609101085344	24.25358967641426	17.52402340767432
34	18.084557911858365	31.908367108771152	26.47373221273132	23.533342766639162
35	17.854726156268782	29.290834889595295	30.9992672843147	21.855171669821218
36	29.414191708124175	25.84756151379341	27.777769994522146	16.960476783560267
37	18.210716702415162	34.28184756431577	30.689088980824703	16.81834675244437
38	17.580763341239564	35.982574535958406	24.12161679389955	22.31504532890248
39	17.633300316762938	34.88833440975759	28.36022992982461	19.118135343654867
40	23.06828545930626	28.757129267578524	24.702885891090148	23.47169938202507
41	15.17632109478651	25.881885671135347	28.53009948401685	30.411693750061293
42	21.042809929628472	26.59575809454694	25.436512217298535	26.924919758526052
43	21.251907092211496	27.008068278454378	26.370549592803417	25.36947503653071
44	17.27226622096632	33.54871158321227	28.720283335411462	20.458738860409955
45	15.257508234295297	42.74009047567678	21.90616756072924	20.096233729298678
46	22.07897918555079	36.73630501146707	23.73193253411751	17.45278326886463
47	19.74843895133395	31.738707702481005	25.15554447219953	23.35730887398551
48	22.010050673664125	27.47326568438867	30.16512021160493	20.351563430342274
49	20.9902029048044	24.709540574656444	32.03655732904818	22.26369919149097
50	18.558091184575705	35.28096074016893	26.449495154689874	19.711452920565495
51	15.983639285329016	38.095401543606386	22.94268706315505	22.978272107909547
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1341.0
1	1548.5
2	1756.0
3	10235.0
4	18714.0
5	13204.0
6	7694.0
7	8276.5
8	8859.0
9	10693.0
10	12527.0
11	13990.5
12	15454.0
13	15432.0
14	15410.0
15	14143.0
16	12876.0
17	11697.0
18	10518.0
19	9461.0
20	8404.0
21	8292.0
22	8180.0
23	8362.0
24	8544.0
25	8618.5
26	8976.5
27	9260.0
28	11820.0
29	14380.0
30	17178.5
31	19977.0
32	22918.0
33	25859.0
34	28925.0
35	31991.0
36	34230.0
37	36469.0
38	46577.0
39	56685.0
40	81874.5
41	107064.0
42	137270.0
43	167476.0
44	168617.0
45	169758.0
46	156218.5
47	142679.0
48	135041.0
49	127403.0
50	118353.5
51	109304.0
52	97403.0
53	85502.0
54	71930.5
55	58359.0
56	50419.0
57	42479.0
58	35931.5
59	29384.0
60	24841.5
61	20299.0
62	17640.0
63	14981.0
64	11151.5
65	7322.0
66	6220.5
67	5119.0
68	4123.0
69	3127.0
70	2337.0
71	1547.0
72	1175.0
73	803.0
74	667.0
75	465.5
76	400.0
77	295.5
78	191.0
79	151.0
80	111.0
81	81.0
82	51.0
83	52.0
84	53.0
85	34.0
86	15.0
87	11.0
88	7.0
89	6.0
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1427566.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.498235774496656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.92220839325691	20.63373549004217
2	8.35048624538979	4.258453342332805
3	2.94061638468586	2.249415896972041
4	1.4852655341857683	1.514866031136098
5	0.9066619345511095	1.1559139887472727
6	0.5480582623726399	0.8384711275283113
7	0.4571986342277719	0.8160430999922311
8	0.32165023801464815	0.6561210884656374
9	0.23740976852535972	0.5448177227725468
>10	2.1527951830127314	11.968659151604166
>50	0.7230584328676447	13.817598995485492
>100	0.9504465996671364	39.598180028458486
>500	0.0033155113941691697	0.5087880417887624
>1k	2.762926161807642E-4	0.07263368472503653
>5k	2.762926161807642E-4	0.3570392960101699
>10k+	2.762926161807642E-4	1.0092630139387717
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14326	1.0035262817971287	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5068	0.35500985593660817	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.004930069783113E-5	0.0	0.0	0.0562495884603584	0.0
2	7.004930069783113E-5	0.0	0.0	0.19473705593997054	0.0
3	7.004930069783113E-5	0.0	0.0	0.28447021013389223	0.0
4	7.004930069783113E-5	0.0	0.0	0.4420811367040123	0.0
5	7.004930069783113E-5	0.0	0.0	0.8043761199131949	0.0
6	7.004930069783113E-5	0.0	0.0	1.238681784239748	0.0
7	7.004930069783113E-5	0.0	0.0	1.5502610737437008	0.0
8	7.004930069783113E-5	0.0	0.0	2.2120168174361114	0.0
9	7.004930069783113E-5	0.0	0.0	2.442198819529185	0.0
10	7.004930069783113E-5	0.0	0.0	2.8492553058842813	0.0
11	7.004930069783113E-5	0.0	0.0	3.3751854555235976	0.0
12	7.004930069783113E-5	0.0	0.0	3.7658504055154016	0.0
13	7.004930069783113E-5	0.0	0.0	3.931306853763679	0.0
14	7.004930069783113E-5	0.0	0.0	3.9882569352310155	0.0
15	7.004930069783113E-5	0.0	0.0	4.081422505159131	0.0
16	7.004930069783113E-5	0.0	0.0	4.309362929629874	0.0
17	7.004930069783113E-5	0.0	0.0	4.57884258941443	0.0
18	7.004930069783113E-5	0.0	0.0	4.914448789057738	0.0
19	7.004930069783113E-5	0.0	0.0	5.097417562480474	0.0
20	7.004930069783113E-5	0.0	0.0	5.278424955483669	0.0
21	7.004930069783113E-5	0.0	0.0	5.550216242191254	0.0
22	7.004930069783113E-5	0.0	0.0	5.818995408968832	0.0
23	7.004930069783113E-5	0.0	0.0	6.145705347423517	0.0
24	7.004930069783113E-5	0.0	0.0	6.368672271544713	0.0
25	7.004930069783113E-5	0.0	0.0	6.568452877134928	0.0
26	7.004930069783113E-5	0.0	0.0	6.755834756501626	0.0
27	7.004930069783113E-5	0.0	0.0	6.941115156847389	0.0
28	7.004930069783113E-5	0.0	0.0	7.147760593905991	0.0
29	7.004930069783113E-5	0.0	0.0	7.35804859460088	0.0
30	7.004930069783113E-5	0.0	0.0	7.6163904155744815	0.0
31	7.004930069783113E-5	0.0	0.0	7.861352820114797	0.0
32	7.004930069783113E-5	0.0	0.0	8.07472299004039	0.0
33	7.004930069783113E-5	0.0	0.0	8.298740653672054	0.0
34	7.004930069783113E-5	0.0	0.0	8.533055564506299	0.0
35	7.004930069783113E-5	0.0	0.0	8.807298576738308	0.0
36	7.004930069783113E-5	0.0	0.0	9.042103832677439	0.0
37	7.004930069783113E-5	0.0	0.0	9.31662704211224	0.0
38	7.004930069783113E-5	0.0	0.0	9.568734475323733	0.0
39	7.004930069783113E-5	0.0	0.0	9.833240634758743	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAAAT	30	2.1658725E-6	45.000004	6
TACGGAG	35	1.2122109E-7	45.000004	1
TCCTTAC	35	1.2122109E-7	45.000004	13
GTGCGTA	35	1.2122109E-7	45.000004	19
ATCGCCG	35	1.2122109E-7	45.000004	22
CGGACCG	30	2.1658725E-6	45.000004	16
GTGCAAC	20	7.033862E-4	45.0	43
CTAGACG	50	2.1827873E-11	45.0	1
GTCGCGA	20	7.033862E-4	45.0	1
TAGACGG	175	0.0	45.0	2
CGCATCA	20	7.033862E-4	45.0	20
GCTGCGC	20	7.033862E-4	45.0	23
TCCTCGA	20	7.033862E-4	45.0	27
TTGTACG	40	6.8175723E-9	45.0	1
GCGTTCC	20	7.033862E-4	45.0	10
ATGTACG	40	6.8175723E-9	45.0	1
AAACGTC	20	7.033862E-4	45.0	32
TGTTGCG	100	0.0	45.0	1
GCCGAAT	20	7.033862E-4	45.0	8
GTTCGCG	25	3.8912818E-5	45.0	1
>>END_MODULE