FastQCFastQC Report
Thu 26 May 2016
SRR1548131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548131_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2013024
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318911.5842334716327278No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC79380.39433210930421103No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT22650.112517287424293No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20800.10332713370531102No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAGT302.1663182E-645.00000418
TAGCGCA302.1663182E-645.0000041
CCGCTCG551.8189894E-1245.00000419
TCTAGCG600.045.0000041
TACGCTT302.1663182E-645.00000433
TCGCTGA551.8189894E-1245.00000413
ACTACGG650.045.0000042
CCCGCCA650.045.00000438
CGAAGTA302.1663182E-645.00000419
GCCCCGT253.891855E-545.09
CTTAACG502.1827873E-1145.01
TCGCACA453.8562575E-1045.018
GTCGAAT207.034553E-445.029
TAGACCG207.034553E-445.011
GCAACCG253.891855E-545.027
ATGTCGC406.8193913E-945.013
AATGCGT253.891855E-545.02
CTAACGT351.2125383E-745.034
GCCGACC502.1827873E-1145.014
CTACGCC207.034553E-445.032