##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548131_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2013024
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07120183365921	31.0	31.0	33.0	30.0	34.0
2	31.426293476878566	31.0	31.0	34.0	30.0	34.0
3	31.43718554771329	31.0	31.0	34.0	30.0	34.0
4	35.28538656270367	37.0	35.0	37.0	33.0	37.0
5	35.13654183954091	37.0	35.0	37.0	32.0	37.0
6	35.19380394868615	37.0	35.0	37.0	32.0	37.0
7	35.7189859634063	37.0	35.0	37.0	35.0	37.0
8	35.736014076334904	37.0	35.0	37.0	35.0	37.0
9	37.488603712623394	39.0	37.0	39.0	35.0	39.0
10	36.74813911806317	39.0	37.0	39.0	32.0	39.0
11	36.307709694469615	38.0	35.0	39.0	32.0	39.0
12	35.229000250369594	37.0	35.0	39.0	30.0	39.0
13	34.710640807064394	37.0	33.0	39.0	27.0	39.0
14	35.53502293067544	37.0	33.0	40.0	27.0	41.0
15	36.02315123913078	37.0	34.0	40.0	30.0	41.0
16	36.2869255408778	37.0	35.0	40.0	31.0	41.0
17	36.19524109002178	37.0	34.0	40.0	31.0	41.0
18	36.11306223870157	37.0	35.0	40.0	31.0	41.0
19	35.98109610218259	37.0	35.0	40.0	30.0	41.0
20	35.7737364283784	36.0	34.0	40.0	30.0	41.0
21	35.543480355922235	35.0	34.0	40.0	30.0	41.0
22	35.43853227780692	35.0	34.0	40.0	30.0	41.0
23	35.4363932074332	35.0	34.0	40.0	30.0	41.0
24	35.2466691902332	35.0	34.0	40.0	29.0	41.0
25	35.11771295324844	35.0	34.0	40.0	29.0	41.0
26	34.9969458883749	35.0	34.0	39.0	29.0	41.0
27	34.91234580412355	35.0	34.0	39.0	29.0	41.0
28	34.96705354729998	35.0	34.0	40.0	29.0	41.0
29	35.04577640405678	35.0	34.0	40.0	29.0	41.0
30	34.948372200232086	36.0	34.0	40.0	29.0	41.0
31	34.7034868933505	35.0	34.0	39.0	27.0	41.0
32	34.43994855500978	35.0	33.0	39.0	27.0	41.0
33	34.2674811626687	35.0	33.0	39.0	25.0	41.0
34	34.10266693293274	35.0	33.0	39.0	24.0	41.0
35	33.903531204794376	35.0	33.0	39.0	24.0	41.0
36	33.64140914365651	35.0	33.0	39.0	23.0	41.0
37	33.50673911488388	35.0	33.0	39.0	23.0	41.0
38	33.4846117085539	35.0	33.0	39.0	23.0	41.0
39	33.509235359339975	35.0	33.0	39.0	23.0	41.0
40	33.37228865627037	35.0	33.0	39.0	23.0	41.0
41	33.36446212265726	35.0	33.0	39.0	23.0	41.0
42	33.31690531260432	35.0	33.0	39.0	23.0	41.0
43	33.197705044748595	35.0	32.0	39.0	22.0	41.0
44	33.06548506128094	35.0	32.0	39.0	22.0	41.0
45	32.948321530195365	35.0	32.0	39.0	22.0	40.0
46	32.83345603430461	35.0	32.0	38.0	21.0	40.0
47	32.78679439490041	35.0	32.0	38.0	21.0	40.0
48	32.699420871286186	35.0	32.0	38.0	20.0	40.0
49	32.64578117300142	35.0	32.0	38.0	20.0	40.0
50	32.445303185655014	35.0	31.0	38.0	20.0	40.0
51	32.04735562020125	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	6.0
14	30.0
15	70.0
16	240.0
17	593.0
18	1300.0
19	2660.0
20	4716.0
21	7688.0
22	11539.0
23	16649.0
24	23895.0
25	33080.0
26	43265.0
27	49212.0
28	52889.0
29	59200.0
30	70282.0
31	85248.0
32	105262.0
33	134171.0
34	225447.0
35	323846.0
36	157778.0
37	153329.0
38	194539.0
39	256030.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.22542205159998	24.760459885227398	29.660699524695183	15.353418538477435
2	31.387752952771553	25.423591571685183	30.921091849873623	12.26756362566964
3	26.742651851145343	24.852609804950166	34.83753795285104	13.567200391053461
4	24.18018861176022	27.896438393183587	33.5192724974963	14.404100497559892
5	22.256962659163527	31.55814337037214	32.33871031840654	13.8461836520578
6	20.58082765034098	38.654382660117314	30.327407919627387	10.437381769914317
7	84.64081898675822	4.012321760694358	9.35214880696902	1.9947104455783935
8	85.51572162080532	3.0415931454369147	9.017676888104663	2.425008345653107
9	81.918546425676	5.127410304099703	10.453029869489882	2.5010134007344176
10	53.988328008011834	21.883842418172858	15.214075937495034	8.913753636320282
11	46.358364331473446	19.89941500945841	22.03351773252579	11.708702926542356
12	40.59931724609344	22.642203967761933	23.88088765956094	12.877591126583688
13	19.736128332300062	44.306376873797824	24.160864450697062	11.796630343205049
14	12.71827856995247	46.863574403484506	27.75466164337832	12.6634853831847
15	10.391083265773284	27.19783768102119	50.25508886133498	12.155990191870538
16	12.837899597819003	22.201225618770565	48.58903818335003	16.37183660006041
17	13.277039916066574	23.492119318994707	29.060557648592365	34.17028311634635
18	18.92838833516143	27.34274405074157	35.139670465925896	18.58919714817111
19	26.35562218830973	29.747136646160204	25.47922925906497	18.418011906465097
20	28.833039248414327	27.315720031157102	25.51589052092772	18.335350199500848
21	19.175479278935573	32.73021086683517	29.06636979986329	19.02794005436597
22	19.940994245473476	27.933248684566102	26.07872534058213	26.04703172937829
23	15.297830527604242	34.13054191107508	25.993430778768655	24.578196782552023
24	17.191101546727708	27.977460775430394	39.27663058165228	15.554807096189613
25	13.717422146978874	29.523592366509295	36.67447581350247	20.08450967300936
26	13.194477562115503	40.413874846996364	27.458291605067796	18.933355985820338
27	14.970959114248018	40.18342553292956	29.26100235267935	15.584613000143069
28	11.583915541990558	34.07555001828095	38.81935833849969	15.521176101228798
29	12.130456467483745	28.84749511183175	39.020349484159155	20.001698936525347
30	16.30228949083568	35.802404740330964	31.605485081151542	16.289820687681818
31	27.272551146931185	31.83469248255361	24.04884392833866	16.843912442176546
32	27.672347671960196	31.835735689191978	25.7587092851352	14.733207353712624
33	25.6425656127299	33.06816014116076	23.13628650229704	18.152987743812293
34	17.51171372025371	33.55886467324781	27.035097445435323	21.894324161063157
35	16.89746371627959	30.929934268046484	30.336796779372726	21.835805236301205
36	28.188635604940625	27.74477601856709	27.903740839652187	16.162847536840097
37	17.69223814519847	34.77633649673327	31.672995453606116	15.858429904462144
38	17.19601952088003	37.203083520117	23.95127926939768	21.64961768960529
39	17.354338547379463	35.76211709348721	28.7343320298218	18.149212329311524
40	21.784539081501265	30.27902300220961	25.993281749248894	21.943156167040236
41	14.973939704643364	26.644590427138475	29.746292145548193	28.635177722669976
42	20.070749280684186	28.06980940117952	26.13605202918594	25.723389288950354
43	20.8230006199628	29.069847155324524	26.40703737262944	23.700114852083235
44	17.27465743081056	35.399230212853894	27.333901632568715	19.99221072376683
45	15.310746419317406	42.259108684248176	22.548116664282194	19.882028232152223
46	21.536603637115107	37.1552450442717	24.097725610822323	17.210425707790865
47	18.865150142273514	32.22614335447565	26.28721763873655	22.621488864514284
48	21.066961943821834	28.38167850954584	30.761431557696284	19.78992798893605
49	20.012727120988124	26.194223218401767	31.579504268205444	22.21354539240466
50	17.250564325114855	35.78909143656509	27.96320361803933	18.99714062028073
51	15.821222201026913	37.2631920930898	24.095589521038992	22.819996184844292
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2550.0
1	2843.0
2	3136.0
3	20500.5
4	37865.0
5	24700.5
6	11536.0
7	13258.0
8	14980.0
9	19256.0
10	23532.0
11	26692.0
12	29852.0
13	30123.5
14	30395.0
15	27363.0
16	24331.0
17	21800.0
18	19269.0
19	16760.0
20	14251.0
21	13690.5
22	13130.0
23	12484.0
24	11838.0
25	12261.0
26	14448.5
27	16213.0
28	18629.0
29	21045.0
30	25157.5
31	29270.0
32	33796.0
33	38322.0
34	43181.5
35	48041.0
36	50820.0
37	53599.0
38	67204.5
39	80810.0
40	115570.0
41	150330.0
42	179770.0
43	209210.0
44	221340.0
45	233470.0
46	229885.0
47	226300.0
48	207823.5
49	189347.0
50	166108.5
51	142870.0
52	123094.0
53	103318.0
54	85494.0
55	67670.0
56	58185.0
57	48700.0
58	42410.0
59	36120.0
60	31098.5
61	26077.0
62	21095.5
63	16114.0
64	13464.5
65	10815.0
66	8230.5
67	5646.0
68	4680.5
69	3715.0
70	3101.0
71	2487.0
72	2119.0
73	1751.0
74	1474.0
75	864.5
76	532.0
77	424.5
78	317.0
79	245.0
80	173.0
81	133.5
82	94.0
83	72.0
84	50.0
85	48.5
86	47.0
87	30.0
88	13.0
89	9.0
90	5.0
91	5.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2013024.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.636315931983926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.23879079535385	18.492767773753798
2	9.200095590666432	4.349127319708882
3	3.413663018608024	2.4205925277944758
4	1.9348299028122968	1.829290034300848
5	1.2128121835676222	1.43332059684818
6	0.839615467387839	1.1907249869135585
7	0.6050570966911201	1.0010924485996182
8	0.44031515382461234	0.832594246835091
9	0.33753900630553474	0.718036073316498
>10	2.2705372388467917	10.863427527830611
>50	0.5122910982430469	8.83331753449047
>100	0.9802771966156186	43.56321653435689
>500	0.011848806870478722	1.7393359388844798
>1k	0.0019042725327555086	0.7412644781663004
>5k	2.1158583697283428E-4	0.39698808714641015
>10k+	2.1158583697283428E-4	1.5949038910539388
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31891	1.5842334716327278	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7938	0.39433210930421103	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2265	0.112517287424293	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2080	0.10332713370531102	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1015387794681037	0.0
2	0.0	0.0	0.0	0.3432646605306246	0.0
3	0.0	0.0	0.0	0.5435106585912538	0.0
4	0.0	0.0	0.0	0.8151417966204079	0.0
5	0.0	0.0	0.0	1.4850791644809003	0.0
6	0.0	0.0	0.0	2.2965945761203046	0.0
7	0.0	0.0	0.0	2.9271384742556474	0.0
8	0.0	0.0	0.0	4.130204110830273	0.0
9	0.0	0.0	0.0	4.577541052665045	0.0
10	0.0	0.0	0.0	5.206544979096126	0.0
11	0.0	0.0	0.0	6.016371389511501	0.0
12	0.0	0.0	0.0	6.594009808129461	0.0
13	0.0	0.0	0.0	6.83037062648036	0.0
14	0.0	0.0	0.0	6.915019393708173	0.0
15	0.0	0.0	0.0	7.0397571017533815	0.0
16	0.0	0.0	0.0	7.345019234743352	0.0
17	0.0	0.0	0.0	7.711184764811547	0.0
18	0.0	0.0	0.0	8.15027540655253	0.0
19	0.0	0.0	0.0	8.377048659131734	0.0
20	0.0	0.0	0.0	8.590558284451651	0.0
21	0.0	0.0	0.0	8.891597914381547	0.0
22	0.0	0.0	0.0	9.197555518463764	0.0
23	0.0	0.0	0.0	9.551947716470345	0.0
24	0.0	0.0	0.0	9.807831401910757	0.0
25	0.0	0.0	0.0	10.008872224076812	0.0
26	0.0	0.0	0.0	10.19982871540528	0.0
27	0.0	0.0	0.0	10.38035314035004	0.0
28	0.0	0.0	0.0	10.582039757101754	0.0
29	4.967650658909183E-5	0.0	0.0	10.794903587836012	0.0
30	4.967650658909183E-5	0.0	0.0	11.049942772664409	0.0
31	4.967650658909183E-5	0.0	0.0	11.298722717662582	0.0
32	4.967650658909183E-5	0.0	0.0	11.515709698443734	0.0
33	4.967650658909183E-5	0.0	0.0	11.73647209372566	0.0
34	4.967650658909183E-5	0.0	0.0	11.961655698094011	0.0
35	9.935301317818366E-5	0.0	0.0	12.234081660228592	0.0
36	9.935301317818366E-5	0.0	0.0	12.477297836488786	0.0
37	9.935301317818366E-5	0.0	0.0	12.707995533088528	0.0
38	1.490295197672755E-4	0.0	0.0	12.949473031618103	0.0
39	1.490295197672755E-4	0.0	0.0	13.198252976616274	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAAGT	30	2.1663182E-6	45.000004	18
TAGCGCA	30	2.1663182E-6	45.000004	1
CCGCTCG	55	1.8189894E-12	45.000004	19
TCTAGCG	60	0.0	45.000004	1
TACGCTT	30	2.1663182E-6	45.000004	33
TCGCTGA	55	1.8189894E-12	45.000004	13
ACTACGG	65	0.0	45.000004	2
CCCGCCA	65	0.0	45.000004	38
CGAAGTA	30	2.1663182E-6	45.000004	19
GCCCCGT	25	3.891855E-5	45.0	9
CTTAACG	50	2.1827873E-11	45.0	1
TCGCACA	45	3.8562575E-10	45.0	18
GTCGAAT	20	7.034553E-4	45.0	29
TAGACCG	20	7.034553E-4	45.0	11
GCAACCG	25	3.891855E-5	45.0	27
ATGTCGC	40	6.8193913E-9	45.0	13
AATGCGT	25	3.891855E-5	45.0	2
CTAACGT	35	1.2125383E-7	45.0	34
GCCGACC	50	2.1827873E-11	45.0	14
CTACGCC	20	7.034553E-4	45.0	32
>>END_MODULE