FastQCFastQC Report
Thu 26 May 2016
SRR1548130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548130_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1683118
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225711.3410230298766932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG600.045.0000041
CGTTTTT116450.043.1644481
GCGTTAG1350.041.6666641
GCGCGAC1250.041.3999989
TACGGGA7750.040.9354864
CGTTAGG3250.040.846152
TCGTTAG950.040.263161
TACGCGG1400.040.178572
TCATACG451.9292202E-840.01
TAATCGG451.9292202E-840.02
ACGGGAT7300.038.8356175
CGACAGG1050.038.571432
ACGTCGT356.2500185E-638.57142629
CGTCGTA356.2500185E-638.57142630
TAAGGGA23600.038.326274
GTTTGCG1000.038.251
TAGGGAC17900.038.212295
CTACGCG659.094947E-1238.0769271
CGGTCTA1250.037.831
CGAGGGA11750.037.7234044
Produced by FastQC (version 0.11.5)