##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548130_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1683118
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.115987708526674	31.0	31.0	33.0	30.0	34.0
2	31.493155560097392	31.0	31.0	34.0	30.0	34.0
3	31.499836612762742	31.0	31.0	34.0	30.0	34.0
4	35.367377688314185	37.0	35.0	37.0	33.0	37.0
5	35.24190044904754	37.0	35.0	37.0	33.0	37.0
6	35.29496505889664	37.0	35.0	37.0	32.0	37.0
7	35.749175637121105	37.0	35.0	37.0	35.0	37.0
8	35.75502846502741	37.0	35.0	37.0	35.0	37.0
9	37.48151941812755	39.0	37.0	39.0	35.0	39.0
10	36.79594122337234	39.0	37.0	39.0	32.0	39.0
11	36.43787007209239	39.0	35.0	39.0	32.0	39.0
12	35.973181915944096	37.0	35.0	39.0	31.0	39.0
13	35.7660894839221	38.0	35.0	39.0	30.0	39.0
14	36.75081426257695	39.0	35.0	41.0	31.0	41.0
15	36.98341471007974	39.0	35.0	41.0	31.0	41.0
16	37.0610444425168	39.0	35.0	41.0	32.0	41.0
17	36.921059604852424	38.0	35.0	41.0	31.0	41.0
18	36.80319621084202	38.0	35.0	40.0	31.0	41.0
19	36.68901586222713	38.0	35.0	40.0	31.0	41.0
20	36.517977943317106	38.0	35.0	40.0	31.0	41.0
21	36.378041824756195	38.0	35.0	40.0	30.0	41.0
22	36.30331919687152	38.0	35.0	40.0	30.0	41.0
23	36.24162536435354	38.0	35.0	40.0	30.0	41.0
24	36.064966330346415	38.0	34.0	40.0	30.0	41.0
25	35.93757181611747	38.0	34.0	40.0	30.0	41.0
26	35.85318914063066	38.0	34.0	40.0	30.0	41.0
27	35.80069252423181	38.0	34.0	40.0	30.0	41.0
28	35.79729585210306	38.0	34.0	40.0	30.0	41.0
29	35.79807892257109	38.0	34.0	40.0	30.0	41.0
30	35.706583852112566	37.0	34.0	40.0	29.0	41.0
31	35.545034275671696	38.0	34.0	40.0	29.0	41.0
32	35.3323641004374	37.0	34.0	40.0	27.0	41.0
33	35.13146315350439	37.0	34.0	40.0	26.0	41.0
34	34.91505289587539	37.0	34.0	40.0	25.0	41.0
35	34.6823003497081	37.0	33.0	40.0	24.0	41.0
36	34.496063258785185	37.0	33.0	40.0	23.0	41.0
37	34.41593162214414	37.0	33.0	40.0	23.0	41.0
38	34.32489997730403	37.0	33.0	40.0	23.0	41.0
39	34.288109924556686	37.0	33.0	40.0	23.0	41.0
40	34.15392206607024	37.0	33.0	40.0	23.0	41.0
41	34.08401728221075	37.0	33.0	40.0	22.0	41.0
42	34.036442483533534	37.0	33.0	40.0	22.0	41.0
43	33.946184402994916	37.0	33.0	40.0	22.0	41.0
44	33.79452361628834	36.0	33.0	40.0	21.0	41.0
45	33.70670386746502	36.0	33.0	40.0	21.0	41.0
46	33.605494683082235	36.0	33.0	40.0	21.0	41.0
47	33.575654232204755	36.0	32.0	40.0	21.0	41.0
48	33.457682705550056	36.0	32.0	40.0	20.0	41.0
49	33.335265263635705	36.0	32.0	40.0	20.0	41.0
50	33.20319728028576	35.0	32.0	40.0	20.0	41.0
51	32.861947290683126	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	4.0
13	12.0
14	26.0
15	71.0
16	193.0
17	517.0
18	1103.0
19	2198.0
20	3788.0
21	5857.0
22	8610.0
23	12450.0
24	17962.0
25	25096.0
26	33590.0
27	39443.0
28	41921.0
29	45817.0
30	51459.0
31	60936.0
32	73489.0
33	92665.0
34	138573.0
35	177258.0
36	137097.0
37	161250.0
38	228027.0
39	323664.0
40	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.53860870123188	23.637142493871494	27.91230323720619	16.91194556769044
2	33.589861198085934	24.69191108407135	29.028683669237687	12.689544048605029
3	27.408773478745996	24.74140256357546	34.69905259167806	13.150771366000482
4	25.058433217397713	27.013198123958038	33.972543814515674	13.955824844128575
5	22.758297398043393	30.924747997466607	32.76288412339479	13.554070481095208
6	21.490471850458494	39.15387988245625	29.21666811239616	10.138980154689095
7	85.19420504088245	3.3256135339292907	9.805610777140997	1.6745706480472553
8	86.30250523136228	2.596549974511591	9.487035371257392	1.6139094228687472
9	81.85480756548263	5.3431785531376885	10.711191966338664	2.0908219150410132
10	41.515984024887146	34.310666275329474	15.604966496704332	8.568383203079048
11	31.51264498389299	22.881818149410798	30.19009956521171	15.415437301484507
12	29.940384453139945	22.468715800080567	31.173750147048512	16.417149599730976
13	22.87350025369582	28.954238502588648	31.456677428439363	16.715583815276172
14	16.764184091667964	33.04438547980593	32.733177352984164	17.45825307554194
15	16.217995410898105	27.200469604626655	41.057905625155215	15.523629359320024
16	20.892296321470035	25.088437055512447	38.267845748188776	15.751420874828739
17	20.004123299732996	24.70331848390903	31.982665505329987	23.309892711027985
18	21.021699013378743	26.431777213481173	34.64866990906164	17.897853864078456
19	24.431263880488473	27.16006839686819	30.728089177348235	17.6805785452951
20	25.81868888574657	27.61184896127307	29.974309584948887	16.595152568031473
21	22.570728849670672	27.082830793800554	32.72889957804503	17.617540778483743
22	21.956689905282932	23.920723324211373	32.593674359135846	21.52891241136985
23	18.936343144093286	27.63383197137693	32.89745579335495	20.532369091174832
24	19.45609279919768	26.7166057281783	35.979176742212964	17.848124730411058
25	19.256641542660706	27.617493247651087	34.028570783510126	19.09729442617808
26	18.88156385945608	32.249729371321564	29.807416948781963	19.061289820440397
27	17.811228921561057	31.21118067776591	32.95490868732911	18.022681713343925
28	17.06267772075398	29.102594114019336	36.53855522904514	17.296172936181538
29	18.085838307236926	26.688265469206556	36.16217044794245	19.063725775614067
30	19.343088244555638	28.50869635997001	33.67719910309319	18.471016292381165
31	23.911335984761614	27.390414694632227	30.151718417841174	18.54653090276499
32	24.789646358722322	27.683264037340223	30.408919636056414	17.118169967881038
33	23.444107899743212	28.35564707881444	29.445766725802947	18.7544782956394
34	19.57622697873827	29.29117269258602	31.275763196638618	19.85683713203709
35	19.56535430076798	28.81176483169926	32.02746331510922	19.595417552423537
36	23.531742872454576	28.239553020049694	29.961535673672312	18.267168433823418
37	20.10269036395547	31.09348245339899	30.507902595064635	18.295924587580906
38	19.774846445703748	31.840667142767177	28.173009854329877	20.211476557199198
39	19.890049301356175	30.57111860249846	29.735467150847416	19.80336494529795
40	21.881056467817466	28.71717847471181	29.058152785485035	20.343612271985684
41	18.505832627302425	28.125835502917802	30.205071777498667	23.163260092281114
42	20.866094949967856	28.46781984388498	28.56490156958692	22.10118363656024
43	21.219367863691076	28.368836884876757	28.863751679917865	21.5480435715143
44	19.449260242003234	30.723930229490744	29.69090699523147	20.135902533274553
45	18.746932775955102	32.63193667942473	28.280607776757183	20.34052276786298
46	21.207782223230932	30.932531171314192	28.37329290043835	19.48639370501652
47	20.24171804947722	29.592280517468172	29.318799989067905	20.847201443986695
48	21.061803153433093	27.94729781274991	30.74407141982915	20.24682761398785
49	20.278792098949687	27.415784276562903	31.73443573177876	20.57098789270865
50	19.127476504915283	30.691787503906443	30.129141272329097	20.051594718849184
51	18.702847928665726	31.517041585913763	29.183634183699535	20.596476301720973
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1301.0
1	1944.0
2	2587.0
3	18121.5
4	33656.0
5	23296.0
6	12936.0
7	12968.0
8	13000.0
9	14054.5
10	15109.0
11	15806.5
12	16504.0
13	16399.5
14	16295.0
15	15159.0
16	14023.0
17	12970.5
18	11918.0
19	11053.0
20	10188.0
21	10416.0
22	10644.0
23	11988.0
24	13332.0
25	14109.5
26	18974.0
27	23061.0
28	25012.5
29	26964.0
30	31935.5
31	36907.0
32	42273.0
33	47639.0
34	53615.0
35	59591.0
36	63079.5
37	66568.0
38	74791.0
39	83014.0
40	94255.5
41	105497.0
42	119493.5
43	133490.0
44	138967.0
45	144444.0
46	140303.0
47	136162.0
48	130253.5
49	124345.0
50	118487.5
51	112630.0
52	102902.0
53	93174.0
54	83348.5
55	73523.0
56	66732.5
57	59942.0
58	55067.0
59	50192.0
60	45320.0
61	40448.0
62	34559.0
63	28670.0
64	24169.5
65	19669.0
66	15806.0
67	11943.0
68	9871.0
69	7799.0
70	6225.0
71	4651.0
72	3713.5
73	2776.0
74	2177.5
75	1308.5
76	1038.0
77	744.0
78	450.0
79	379.5
80	309.0
81	215.5
82	122.0
83	107.5
84	93.0
85	58.5
86	24.0
87	18.0
88	12.0
89	9.0
90	6.0
91	4.0
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1683118.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.85799251812815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.58118542734476	19.782285119367053
2	6.105148996596976	3.035234961589304
3	2.282519285468696	1.7021654196199216
4	1.268325416932084	1.2611209489859803
5	0.9175853503190947	1.1404664886488025
6	0.6514277571474654	0.9715911787963617
7	0.5303766285021853	0.9228868784168146
8	0.4519653845997459	0.8987961719066717
9	0.3816462792520039	0.8538264318795985
>10	6.226847780103157	38.382293606831084
>50	1.4399203017466082	24.29686565011272
>100	0.1611274818202215	5.029707488287087
>500	9.619550834890735E-4	0.1492125789532356
>1k	7.214663126168051E-4	0.22423733319454597
>5k	0.0	0.0
>10k+	2.4048877087226838E-4	1.3493097434108496
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22571	1.3410230298766932	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1882708164252299E-4	0.0	0.0	0.02435955173671721	0.0
2	1.1882708164252299E-4	0.0	0.0	0.08276306236401726	0.0
3	1.1882708164252299E-4	0.0	0.0	0.15239573220653574	0.0
4	1.1882708164252299E-4	0.0	0.0	0.21905772500799112	0.0
5	1.1882708164252299E-4	0.0	0.0	0.39795189642080947	0.0
6	1.1882708164252299E-4	0.0	0.0	0.6984061723539289	0.0
7	1.1882708164252299E-4	0.0	0.0	0.9207316421070894	0.0
8	1.1882708164252299E-4	0.0	0.0	1.2829165869534993	0.0
9	1.1882708164252299E-4	0.0	0.0	1.4625831343969942	0.0
10	1.1882708164252299E-4	0.0	0.0	1.670411700189767	0.0
11	1.1882708164252299E-4	0.0	0.0	1.8752695889414766	0.0
12	1.1882708164252299E-4	0.0	0.0	2.034676118964921	0.0
13	1.1882708164252299E-4	0.0	0.0	2.1068041575219327	0.0
14	1.1882708164252299E-4	0.0	0.0	2.1424522820146894	0.0
15	1.1882708164252299E-4	0.0	0.0	2.184220001212036	0.0
16	1.1882708164252299E-4	0.0	0.0	2.2673989583618024	0.0
17	1.7824062246378448E-4	0.0	0.0	2.373452128727754	0.0
18	1.7824062246378448E-4	0.0	0.0	2.508617934096124	0.0
19	1.7824062246378448E-4	0.0	0.0	2.5831225142859857	0.0
20	1.7824062246378448E-4	0.0	0.0	2.663033726690583	0.0
21	1.7824062246378448E-4	0.0	0.0	2.7606501742599154	0.0
22	1.7824062246378448E-4	0.0	0.0	2.8808437673413274	0.0
23	1.7824062246378448E-4	0.0	0.0	3.0079293311580058	0.0
24	1.7824062246378448E-4	0.0	0.0	3.102515688145454	0.0
25	1.7824062246378448E-4	0.0	0.0	3.1867046754891812	0.0
26	1.7824062246378448E-4	0.0	0.0	3.2688141889041646	0.0
27	1.7824062246378448E-4	0.0	0.0	3.3568650564012743	0.0
28	1.7824062246378448E-4	0.0	0.0	3.449966074868191	0.0
29	1.7824062246378448E-4	0.0	0.0	3.553345635897186	0.0
30	1.7824062246378448E-4	0.0	0.0	3.6860754860918843	0.0
31	1.7824062246378448E-4	0.0	0.0	3.803714296917982	0.0
32	1.7824062246378448E-4	0.0	0.0	3.9096486402022914	0.0
33	1.7824062246378448E-4	0.0	0.0	4.022534367762688	0.0
34	1.7824062246378448E-4	0.0	0.0	4.143262682711492	0.0
35	1.7824062246378448E-4	0.0	0.0	4.278428488079861	0.0
36	1.7824062246378448E-4	0.0	0.0	4.4134160528257675	0.0
37	1.7824062246378448E-4	0.0	0.0	4.5452546999081465	0.0
38	1.7824062246378448E-4	0.0	0.0	4.689332536399705	0.0
39	1.7824062246378448E-4	0.0	0.0	4.87042500882291	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	60	0.0	45.000004	1
CGTTTTT	11645	0.0	43.164448	1
GCGTTAG	135	0.0	41.666664	1
GCGCGAC	125	0.0	41.399998	9
TACGGGA	775	0.0	40.935486	4
CGTTAGG	325	0.0	40.84615	2
TCGTTAG	95	0.0	40.26316	1
TACGCGG	140	0.0	40.17857	2
TCATACG	45	1.9292202E-8	40.0	1
TAATCGG	45	1.9292202E-8	40.0	2
ACGGGAT	730	0.0	38.835617	5
CGACAGG	105	0.0	38.57143	2
ACGTCGT	35	6.2500185E-6	38.571426	29
CGTCGTA	35	6.2500185E-6	38.571426	30
TAAGGGA	2360	0.0	38.32627	4
GTTTGCG	100	0.0	38.25	1
TAGGGAC	1790	0.0	38.21229	5
CTACGCG	65	9.094947E-12	38.076927	1
CGGTCTA	125	0.0	37.8	31
CGAGGGA	1175	0.0	37.723404	4
>>END_MODULE