FastQCFastQC Report
Thu 26 May 2016
SRR1548129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548129_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences448805
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76081.695168280210782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCTA253.8869923E-545.024
TAGGACG207.028687E-445.01
CCAGTCG207.028687E-445.033
TCGATAG302.1625347E-644.9999961
CGTTTTT44650.043.8409841
ACGGGAT2250.041.05
CGGGATA1100.040.9090926
TACGGGA1550.040.645164
CTACGGG1000.040.53
TACGGCG850.039.70588315
CGCAGTA403.4519508E-739.37537
ATAGGGA5150.038.88354
CGAGGGA3000.038.2500044
CGGGACC1300.038.076926
TCGTAGG659.094947E-1238.076922
TAGGGAC4050.037.7777755
TAAGGGA5900.037.754244
TAGGGAT7350.037.653065
ACGGGAG1500.037.5000045
CGTATGG301.1388135E-437.4999962
Produced by FastQC (version 0.11.5)