FastQCFastQC Report
Thu 26 May 2016
SRR1548128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548128_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences319228
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13190.4131843071409776No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5300.16602553660706454No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC551.8189894E-1245.025
GAATGCG207.025623E-445.01
CGACGGT551.8189894E-1245.028
GTAGGGT1400.045.04
CACGACG551.8189894E-1245.026
TATTGCG207.025623E-445.01
TACGCGG207.025623E-445.02
GCGATCA351.2082455E-745.09
GATTGCG207.025623E-445.01
TTGCACG351.2082455E-745.01
GGACACG207.025623E-445.08
TTCGCAT351.2082455E-745.025
TAATGCG207.025623E-445.01
ATAGCGG600.044.9999962
TAACGTA253.8844555E-544.99999618
TCGATCA302.1605592E-644.99999617
GCGATAT302.1605592E-644.9999969
CCCCACG302.1605592E-644.99999638
CGTTTTT7550.042.9139061
GGATGTA900.042.5000048
Produced by FastQC (version 0.11.5)