##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548128_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	319228
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.144576916811808	31.0	31.0	33.0	30.0	34.0
2	31.53211810994023	31.0	31.0	34.0	30.0	34.0
3	31.661868633077297	31.0	31.0	34.0	30.0	34.0
4	35.47159083789643	37.0	35.0	37.0	33.0	37.0
5	35.31740010274788	37.0	35.0	37.0	33.0	37.0
6	35.35249727467515	37.0	35.0	37.0	33.0	37.0
7	35.74946120014535	37.0	35.0	37.0	35.0	37.0
8	35.741332213966196	37.0	35.0	37.0	35.0	37.0
9	37.47530918340497	39.0	37.0	39.0	35.0	39.0
10	36.80417444585061	39.0	37.0	39.0	32.0	39.0
11	36.45451213552696	39.0	35.0	39.0	32.0	39.0
12	35.74058040021552	37.0	35.0	39.0	31.0	39.0
13	35.41781109426491	37.0	35.0	39.0	30.0	39.0
14	36.29533436916561	38.0	35.0	40.0	30.0	41.0
15	36.61825403786635	38.0	35.0	40.0	31.0	41.0
16	36.76384903579886	38.0	35.0	40.0	31.0	41.0
17	36.653260365632086	38.0	35.0	40.0	31.0	41.0
18	36.59146127532673	38.0	35.0	40.0	31.0	41.0
19	36.524402621323944	38.0	35.0	40.0	31.0	41.0
20	36.40731076221384	38.0	35.0	40.0	31.0	41.0
21	36.25475522197301	38.0	34.0	40.0	30.0	41.0
22	36.15266831230343	38.0	34.0	40.0	30.0	41.0
23	36.145532346786624	38.0	34.0	40.0	30.0	41.0
24	35.96937611988923	38.0	34.0	40.0	30.0	41.0
25	35.85284498853484	37.0	34.0	40.0	30.0	41.0
26	35.782027265778694	37.0	34.0	40.0	30.0	41.0
27	35.77159898254539	37.0	34.0	40.0	30.0	41.0
28	35.827728144147756	37.0	34.0	40.0	30.0	41.0
29	35.86528750610849	37.0	34.0	40.0	30.0	41.0
30	35.80469758291879	37.0	34.0	40.0	30.0	41.0
31	35.65752690866716	37.0	34.0	40.0	29.0	41.0
32	35.52424286090193	37.0	34.0	40.0	29.0	41.0
33	35.43711391231346	37.0	34.0	40.0	29.0	41.0
34	35.344067562995725	37.0	34.0	40.0	28.0	41.0
35	35.19585061460774	37.0	34.0	40.0	27.0	41.0
36	35.02776385530091	37.0	34.0	40.0	26.0	41.0
37	34.939500920971845	37.0	34.0	40.0	26.0	41.0
38	34.88590599822071	37.0	34.0	40.0	26.0	41.0
39	34.85347463255103	37.0	34.0	40.0	26.0	41.0
40	34.7049444284336	37.0	33.0	40.0	25.0	41.0
41	34.64841429949754	36.0	33.0	40.0	25.0	41.0
42	34.622774318042275	36.0	33.0	40.0	25.0	41.0
43	34.51716014885912	36.0	33.0	40.0	24.0	41.0
44	34.40882378738707	36.0	33.0	40.0	24.0	41.0
45	34.29887102635107	36.0	33.0	40.0	24.0	41.0
46	34.20748179984212	36.0	33.0	40.0	24.0	41.0
47	34.16901399626599	36.0	33.0	40.0	24.0	41.0
48	34.05944027466262	35.0	33.0	40.0	23.0	41.0
49	33.96295124487827	36.0	33.0	40.0	24.0	41.0
50	33.808440988885685	35.0	33.0	39.0	24.0	41.0
51	33.421319558434725	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	3.0
15	18.0
16	32.0
17	90.0
18	167.0
19	332.0
20	565.0
21	891.0
22	1302.0
23	2043.0
24	2686.0
25	3690.0
26	5011.0
27	5932.0
28	6883.0
29	8101.0
30	9649.0
31	11818.0
32	14585.0
33	18825.0
34	29600.0
35	39453.0
36	24402.0
37	30319.0
38	42422.0
39	60395.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.131579936597035	23.962183768341124	30.006453068026612	13.899783227035222
2	29.51307529414713	25.86677860338066	31.314608994198505	13.30553710827371
3	28.6199832094929	25.896851153407596	31.153282293533145	14.329883343566355
4	26.198516420865335	27.97498966256093	30.859135163582142	14.967358752991592
5	23.90454471412282	32.55980051875149	28.94514265665919	14.5905121104665
6	22.916536143446066	40.57037603217763	25.85362186274387	10.659465961632439
7	88.39450173543673	3.9561066071898456	5.735399150450462	1.9139925069229518
8	89.41195634468154	3.0288696480258626	5.618241507637175	1.940932499655419
9	84.9007605849111	5.511734559625095	6.889433257734284	2.6980715977295224
10	46.16825591740073	31.660443319508314	12.353552946483392	9.817747816607564
11	39.53631886927212	20.737216033681253	24.654792186149084	15.07167291089754
12	36.10867467765985	21.633753931359404	26.23328780683399	16.024283584146755
13	24.34216296816069	32.61712631724034	26.48733820341574	16.553372511183227
14	17.45742854636811	36.69352312453795	28.6199832094929	17.229065119601035
15	16.121706116004862	26.185359680228554	42.05677446840503	15.63615973536156
16	20.345019860413245	23.759820567118172	39.11498991316551	16.78016965930307
17	21.196135677321536	22.128697983886127	28.43986116506071	28.23530517373163
18	22.94378939190798	25.6067763479394	32.373100104000905	19.076334156151717
19	27.004210157003772	26.972258072600148	26.649917926998885	19.373613843397195
20	29.181024220933	26.580375155061585	26.499868432593633	17.73873219141178
21	24.559562444397105	26.749219993233673	29.61864247497087	19.07257508739835
22	23.549939228388485	22.99547658726678	29.28439861165061	24.170185572694123
23	19.96535391632313	28.05549638502888	28.75593619607303	23.223213502574964
24	20.72656533888005	25.431979650907817	35.13288308043154	18.708571929780597
25	19.752026764569525	26.902402044933403	32.9917801696593	20.35379102083777
26	18.920959314345858	32.672885837081964	27.593757439823573	20.812397408748605
27	18.158181613141704	31.862493265001817	31.19431879409074	18.785006327765736
28	16.834049644768005	28.20053378776298	36.17602465949103	18.789391907977997
29	18.06263861566028	26.310975227736915	35.37753580512988	20.248850351472928
30	20.531093763704938	28.539789742754394	31.128535090906812	19.800581402633853
31	26.65524327439949	26.653363740022805	27.43618980791159	19.255203177666118
32	27.502600022554414	26.915872041299636	27.308694726026538	18.272833210119412
33	25.513112884834666	27.313080306238803	26.536832608668416	20.636974200258123
34	20.891024596839877	27.62978184871001	29.031287982257197	22.447905572192916
35	20.683022792486874	26.809678349017002	31.209041813374768	21.298257045121353
36	26.691580939015374	25.893405340383673	28.518488353151977	18.896525367448973
37	20.521069580362624	30.569060358113948	29.661871765634594	19.24799829588883
38	20.657022566942747	31.59935845226609	26.05410552959014	21.689513451201023
39	21.164183592917915	29.74582430112647	28.280100743042592	20.809891362913028
40	23.672735474331827	26.77647324169559	26.9838485345897	22.566942749382886
41	19.536820078439234	24.638816143947274	29.199506308970392	26.624857468643103
42	22.22612051574423	26.237986642775695	26.909293670981242	24.626599170498828
43	22.09110729635245	26.018394376433147	27.779518087385817	24.110980239828585
44	20.217838034257646	29.746764068314807	28.616850652198426	21.418547245229117
45	19.291540842282007	33.294385204305385	25.79911536582004	21.614958587592568
46	22.024070570250732	30.999786986103977	26.86543786885862	20.110704574786674
47	20.921410402596262	27.97686919693761	28.610585537609484	22.49113486285664
48	22.444146503439548	25.83827233200095	30.657398473818088	21.060182690741414
49	21.13003871840816	24.754720763842773	31.772902126379893	22.34233839136918
50	20.49037051887679	29.925946345558664	28.241883544050022	21.34179959151453
51	18.788138885060206	31.906975578583328	27.092548272707905	22.21233726364855
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	241.0
1	376.0
2	511.0
3	1376.5
4	2242.0
5	1632.5
6	1023.0
7	1056.5
8	1090.0
9	1185.5
10	1281.0
11	1316.0
12	1351.0
13	1317.0
14	1283.0
15	1201.0
16	1119.0
17	1019.0
18	919.0
19	1102.5
20	1286.0
21	1280.5
22	1275.0
23	1480.5
24	1686.0
25	2039.5
26	2933.0
27	3473.0
28	4126.5
29	4780.0
30	5659.5
31	6539.0
32	7288.0
33	8037.0
34	9176.0
35	10315.0
36	11196.0
37	12077.0
38	13355.5
39	14634.0
40	18499.0
41	22364.0
42	25417.0
43	28470.0
44	28788.0
45	29106.0
46	28033.0
47	26960.0
48	25834.0
49	24708.0
50	23505.0
51	22302.0
52	21095.5
53	19889.0
54	17638.0
55	15387.0
56	13779.0
57	12171.0
58	11265.0
59	10359.0
60	9748.0
61	9137.0
62	7976.0
63	6815.0
64	5793.5
65	4772.0
66	4026.5
67	3281.0
68	2639.5
69	1998.0
70	1806.5
71	1615.0
72	1327.0
73	1039.0
74	835.5
75	488.5
76	345.0
77	250.5
78	156.0
79	130.0
80	104.0
81	69.5
82	35.0
83	25.0
84	15.0
85	9.0
86	3.0
87	5.0
88	7.0
89	4.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	319228.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.357925996466474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.32821867486663	21.957347099878458
2	5.456552919689199	3.312992594634556
3	2.938778879590552	2.6764569523976594
4	2.1782872945279688	2.64513137945293
5	1.73561308829751	2.6344806846517224
6	1.5220150447317642	2.7723132056085302
7	1.3383413647573548	2.8440487676519606
8	1.2444407755569544	3.0222912777074695
9	1.0906914591738812	2.980001754232085
>10	10.118562392298088	53.63877855325973
>50	0.043338733477107864	0.8194769882341147
>100	0.003095623819793419	0.11747089854273435
>500	0.0010318746065978062	0.16602553660706454
>1k	0.0010318746065978062	0.4131843071409776
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1319	0.4131843071409776	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	530	0.16602553660706454	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.034144874509754786	0.0
2	0.0	0.0	0.0	0.1005550891525806	0.0
3	0.0	0.0	0.0	0.16132670066535518	0.0
4	0.0	0.0	0.0	0.25154435074617515	0.0
5	0.0	0.0	0.0	0.44513639154460133	0.0
6	0.0	0.0	0.0	0.6916686506196198	0.0
7	0.0	0.0	0.0	0.8442241908604509	0.0
8	0.0	0.0	0.0	1.1888054932524716	0.0
9	0.0	0.0	0.0	1.3253849912914908	0.0
10	0.0	0.0	0.0	1.566278647236458	0.0
11	0.0	0.0	0.0	1.8256543912188155	0.0
12	0.0	0.0	0.0	2.0439936346435776	0.0
13	0.0	0.0	0.0	2.1282594258648992	0.0
14	0.0	0.0	0.0	2.1674163920458107	0.0
15	0.0	0.0	0.0	2.2234891676168758	0.0
16	0.0	0.0	0.0	2.3447191349129777	0.0
17	0.0	0.0	0.0	2.4787925871164185	0.0
18	0.0	0.0	0.0	2.6476374252885084	0.0
19	0.0	0.0	0.0	2.7409876326638014	0.0
20	0.0	0.0	0.0	2.849060859323117	0.0
21	0.0	0.0	0.0	2.9781222198554014	0.0
22	0.0	0.0	0.0	3.1168945080005512	0.0
23	0.0	0.0	0.0	3.2710163268886188	0.0
24	0.0	0.0	0.0	3.3837883894896437	0.0
25	0.0	0.0	0.0	3.4837169671833297	0.0
26	0.0	0.0	0.0	3.5823925219592265	0.0
27	0.0	0.0	0.0	3.6788752866289927	0.0
28	0.0	0.0	0.0	3.780370142969915	0.0
29	0.0	0.0	0.0	3.9034796446427005	0.0
30	0.0	0.0	0.0	4.052589371859611	0.0
31	0.0	0.0	0.0	4.180397709474106	0.0
32	0.0	0.0	0.0	4.301940932499655	0.0
33	0.0	0.0	0.0	4.417532296665706	0.0
34	0.0	0.0	0.0	4.554111794704725	0.0
35	0.0	0.0	0.0	4.706040823486662	0.0
36	0.0	0.0	0.0	4.847945668926284	0.0
37	0.0	0.0	0.0	4.987657724259777	0.0
38	0.0	0.0	0.0	5.155249539514077	0.0
39	0.0	0.0	0.0	5.348841580312504	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	55	1.8189894E-12	45.0	25
GAATGCG	20	7.025623E-4	45.0	1
CGACGGT	55	1.8189894E-12	45.0	28
GTAGGGT	140	0.0	45.0	4
CACGACG	55	1.8189894E-12	45.0	26
TATTGCG	20	7.025623E-4	45.0	1
TACGCGG	20	7.025623E-4	45.0	2
GCGATCA	35	1.2082455E-7	45.0	9
GATTGCG	20	7.025623E-4	45.0	1
TTGCACG	35	1.2082455E-7	45.0	1
GGACACG	20	7.025623E-4	45.0	8
TTCGCAT	35	1.2082455E-7	45.0	25
TAATGCG	20	7.025623E-4	45.0	1
ATAGCGG	60	0.0	44.999996	2
TAACGTA	25	3.8844555E-5	44.999996	18
TCGATCA	30	2.1605592E-6	44.999996	17
GCGATAT	30	2.1605592E-6	44.999996	9
CCCCACG	30	2.1605592E-6	44.999996	38
CGTTTTT	755	0.0	42.913906	1
GGATGTA	90	0.0	42.500004	8
>>END_MODULE