FastQCFastQC Report
Thu 26 May 2016
SRR1548127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548127_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4644084
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1045702.251681924788613No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT54100.1164922942823601No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCT49690.1069963420127629No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT480300.043.9178661
CGGTCTA4950.041.36363631
TCCGCGT556.184564E-1140.90909242
CGACGGT5150.039.3203928
CACGACG5200.038.94230726
AGGGCGA25750.038.796126
GACCGAT13300.038.571439
GGGCGAT49050.038.3944937
GTTTTTT560000.038.3303572
TAGGGAC53250.037.7746475
TACGGGA19400.037.461344
TAACGGG11600.037.2413833
GGACCGA16350.037.1559648
TAAGGGA60500.036.8925634
ATAGGGA71850.036.6701434
TTAGGGA66600.036.5878374
ACGGGAC17100.036.578955
CGTAAGG8150.036.4417152
TATGGGA46250.036.2918934
TAGGGAT78700.036.2229965
Produced by FastQC (version 0.11.5)