##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548127_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4644084
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19772618238602	31.0	31.0	33.0	30.0	34.0
2	31.573281404901376	31.0	31.0	34.0	30.0	34.0
3	31.485557754769292	31.0	31.0	34.0	30.0	34.0
4	35.39902680485538	37.0	35.0	37.0	33.0	37.0
5	35.30662106886955	37.0	35.0	37.0	33.0	37.0
6	35.37008891312043	37.0	35.0	37.0	33.0	37.0
7	35.81588274458429	37.0	35.0	37.0	35.0	37.0
8	35.830383343625996	37.0	35.0	37.0	35.0	37.0
9	37.57150710452266	39.0	37.0	39.0	35.0	39.0
10	36.91730209875618	39.0	37.0	39.0	32.0	39.0
11	36.57752120762674	39.0	35.0	39.0	32.0	39.0
12	36.23803251620772	38.0	35.0	39.0	32.0	39.0
13	36.10713156781833	38.0	35.0	39.0	31.0	39.0
14	37.09795537720679	39.0	35.0	41.0	31.0	41.0
15	37.27801994968222	39.0	35.0	41.0	32.0	41.0
16	37.32766160129748	39.0	35.0	41.0	32.0	41.0
17	37.17984773746556	39.0	35.0	41.0	32.0	41.0
18	36.99214355295899	39.0	35.0	40.0	31.0	41.0
19	36.81996815733738	38.0	35.0	40.0	31.0	41.0
20	36.593368896858884	38.0	35.0	40.0	31.0	41.0
21	36.4702972642183	38.0	35.0	40.0	31.0	41.0
22	36.411801121599005	38.0	35.0	40.0	31.0	41.0
23	36.333541555234575	38.0	35.0	40.0	31.0	41.0
24	36.17754093164551	38.0	35.0	40.0	30.0	41.0
25	36.06667364328466	38.0	35.0	40.0	30.0	41.0
26	36.01039968269308	38.0	35.0	40.0	30.0	41.0
27	35.96893144051658	38.0	35.0	40.0	30.0	41.0
28	35.94642517232677	38.0	35.0	40.0	30.0	41.0
29	35.913449455263944	38.0	35.0	40.0	30.0	41.0
30	35.76549605907214	38.0	34.0	40.0	29.0	41.0
31	35.56791651486063	38.0	34.0	40.0	29.0	41.0
32	35.29184269707439	38.0	34.0	40.0	27.0	41.0
33	35.000687326069034	38.0	34.0	40.0	25.0	41.0
34	34.65492893754721	38.0	34.0	40.0	23.0	41.0
35	34.35168183865753	37.0	33.0	40.0	22.0	41.0
36	34.14876324373117	37.0	33.0	40.0	21.0	41.0
37	34.06668139508243	37.0	33.0	40.0	21.0	41.0
38	33.9417738783364	37.0	33.0	40.0	20.0	41.0
39	33.88168065004854	37.0	33.0	40.0	19.0	41.0
40	33.77692328562532	37.0	33.0	40.0	18.0	41.0
41	33.68804138770961	37.0	33.0	40.0	18.0	41.0
42	33.63379602952918	37.0	33.0	40.0	18.0	41.0
43	33.53616622782878	37.0	33.0	40.0	18.0	41.0
44	33.38941802086267	37.0	32.0	40.0	18.0	41.0
45	33.31457096813925	36.0	32.0	40.0	18.0	41.0
46	33.21406826405379	36.0	32.0	40.0	18.0	41.0
47	33.18134749500655	36.0	32.0	40.0	18.0	41.0
48	33.062530522703725	36.0	32.0	40.0	18.0	41.0
49	32.96132498895369	36.0	31.0	40.0	18.0	41.0
50	32.836754675410695	35.0	31.0	40.0	17.0	41.0
51	32.512374883830695	35.0	31.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	2.0
11	4.0
12	10.0
13	20.0
14	65.0
15	211.0
16	493.0
17	1289.0
18	3019.0
19	5808.0
20	10132.0
21	15959.0
22	24304.0
23	36404.0
24	53451.0
25	79205.0
26	108625.0
27	124943.0
28	125513.0
29	127717.0
30	139434.0
31	161759.0
32	194276.0
33	242675.0
34	355564.0
35	452502.0
36	377456.0
37	447691.0
38	634482.0
39	920907.0
40	161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.826420021687806	21.93696324183628	27.659469553091633	20.57714718338428
2	36.41785979754027	23.384137754614258	28.062240045615024	12.13576240223045
3	26.681774059211676	23.87495575015439	37.047047383294526	12.396222807339402
4	23.982555009771573	25.67332976750636	36.8434980934884	13.500617129233666
5	22.04236615875165	29.593801490240057	35.396280515167255	12.96755183584104
6	20.529839684208984	37.59787721324593	31.695507660929472	10.176775441615613
7	81.54387388341813	3.1422773576016283	13.649946900185267	1.6639018587949743
8	82.63603759105132	2.4203050590816186	13.321529067949674	1.6221282819173812
9	78.0448630989448	5.036450675741437	14.581669926728285	2.3370162985854694
10	37.76945033724627	33.383870748246586	19.494823952366065	9.351854962141081
11	27.24675092009533	23.499338082601433	33.90300433842282	15.350906658880417
12	27.044472063812798	21.556995954422874	34.82680330502205	16.571728676742282
13	22.407971087516938	25.437287525376373	34.97596081380095	17.178780573305737
14	16.845194875889412	28.918512240519334	36.61621968939407	17.62007319419718
15	16.769614847621188	26.30703923529376	41.38773114353659	15.535614773548453
16	21.365569615019883	25.206542345056636	38.46093223119995	14.966955808723528
17	20.882374220621333	24.765960305627548	34.398774871427825	19.9528906023233
18	20.723785357887582	25.626517522077553	36.584717244563194	17.064979875471675
19	22.79426900977674	26.890555812513295	33.53991874393314	16.77525643377682
20	23.55405285520245	27.707336904328173	32.9000939690152	15.838516271454179
21	22.80507846111311	25.98654546300196	35.33454605902907	15.873830016855853
22	21.459430966364952	23.328820064408827	36.20130471369596	19.010444255530263
23	18.60409932292353	26.616874285650304	36.03005458126942	18.74897181015675
24	19.09476658906256	25.650052841421473	37.79942395529452	17.45575661422145
25	19.46103903374702	26.992642682604362	35.25353977232108	18.29277851132753
26	18.167307051293648	29.922606912364202	33.473899266249276	18.436186770092874
27	17.294023966836086	28.931690296730206	35.848727111740445	17.925558624693267
28	16.490700857262702	27.873548368203505	37.66682514786554	17.96892562666825
29	18.17103222077809	26.367352528507237	37.42139461732389	18.04022063339078
30	19.048449597380237	27.071086569493573	36.31495468212892	17.56550915099727
31	21.781733491470007	26.366943405847092	33.96336069717947	17.887962405503433
32	22.6502147678638	27.103902513391233	33.19011886951226	17.0557638492327
33	21.60798555753944	27.731927329479827	32.76467436850841	17.89541274447232
34	19.20671546854019	28.498321735782557	33.84641621469379	18.448546580983464
35	19.167095168821234	29.097686432889674	33.250884350929056	18.48433404736004
36	21.580746601482662	29.341028284587445	31.2927156356345	17.785509478295396
37	20.348985935654913	30.690164088332594	31.203591494038434	17.757258481974056
38	19.996214538755115	31.171098541714574	29.72917802520368	19.10350889432663
39	19.753195678631137	30.100876728327915	30.305287328997494	19.840640264043458
40	21.40882895313694	28.21964460591152	30.29213080555821	20.07939563539333
41	18.59436651016648	28.294578651032154	30.977282064665495	22.133772774135867
42	19.85511028654951	28.682340801759832	30.18829978096865	21.274249130722012
43	19.956852632295195	28.649632521720108	30.172559324938998	21.2209555210457
44	19.436879264027095	29.732300277083706	30.55483061891215	20.275989839977054
45	18.95923932469783	30.68930277746914	29.97854905294564	20.372908844887387
46	20.384665738173556	29.742377614186132	30.295597581783618	19.57735906585669
47	19.496546574092974	29.032528266069264	31.1965287449581	20.27439641487966
48	19.732653414537722	28.06549149412457	32.213177022637836	19.988678068699876
49	20.330812276436	27.16613222327589	32.3274514414468	20.175604058841312
50	18.869253872238314	29.144240285059443	31.64443192672656	20.342073915975682
51	18.209640480232487	29.72736927239042	31.365819395170284	20.69717085220681
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3597.0
1	5689.0
2	7781.0
3	81690.5
4	155600.0
5	104919.0
6	54238.0
7	53064.0
8	51890.0
9	53445.5
10	55001.0
11	55615.0
12	56229.0
13	55441.0
14	54653.0
15	51454.0
16	48255.0
17	44910.5
18	41566.0
19	38578.0
20	35590.0
21	35720.0
22	35850.0
23	37051.0
24	38252.0
25	42632.0
26	54489.5
27	61967.0
28	70944.0
29	79921.0
30	90534.5
31	101148.0
32	117159.5
33	133171.0
34	144039.5
35	154908.0
36	167598.0
37	180288.0
38	197357.0
39	214426.0
40	241722.0
41	269018.0
42	302358.5
43	335699.0
44	351714.0
45	367729.0
46	364175.5
47	360622.0
48	341453.5
49	322285.0
50	304826.5
51	287368.0
52	265509.5
53	243651.0
54	223640.0
55	203629.0
56	185322.5
57	167016.0
58	154997.5
59	142979.0
60	127884.5
61	112790.0
62	99291.5
63	85793.0
64	69486.5
65	53180.0
66	42285.5
67	31391.0
68	26256.5
69	21122.0
70	16775.0
71	12428.0
72	9716.5
73	7005.0
74	5587.5
75	3380.5
76	2591.0
77	1780.5
78	970.0
79	724.5
80	479.0
81	421.5
82	364.0
83	265.5
84	167.0
85	145.5
86	124.0
87	121.5
88	119.0
89	70.0
90	21.0
91	18.0
92	15.0
93	13.0
94	11.0
95	6.0
96	1.0
97	2.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4644084.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.975412587297818
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.48010144591574	18.490635357874975
2	6.691755929406093	3.074917068232031
3	2.349263319068367	1.6192588209540122
4	1.3537425631959614	1.244111757056532
5	0.9302361819794951	1.0686280042305776
6	0.6985962672048152	0.9630322482586067
7	0.5556959574387699	0.8937140726674456
8	0.433112172666616	0.7960744690877178
9	0.3356312315623662	0.6940139420095431
>10	4.075368388974372	22.456472462257512
>50	1.4178858914575483	23.04359169045679
>100	0.6743291655667053	22.14675340003151
>500	0.0023786030907389307	0.35904647515534655
>1k	0.0017125942252266368	0.7456143817639914
>5k	9.514412362370205E-5	0.11826127430716801
>10k+	9.514412362370205E-5	2.2858745756562953
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	104570	2.251681924788613	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT	5410	0.1164922942823601	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCT	4969	0.1069963420127629	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.306554317277638E-5	0.0	0.0	0.028315594636100467	0.0
2	4.306554317277638E-5	0.0	0.0	0.09274164722257393	0.0
3	4.306554317277638E-5	0.0	0.0	0.18074608469614245	0.0
4	4.306554317277638E-5	0.0	0.0	0.26190310080523954	0.0
5	4.306554317277638E-5	0.0	0.0	0.486748301710305	0.0
6	4.306554317277638E-5	0.0	0.0	0.9222916725881789	0.0
7	4.306554317277638E-5	0.0	0.0	1.2805754590140919	0.0
8	4.306554317277638E-5	0.0	0.0	1.8215002140357495	0.0
9	4.306554317277638E-5	0.0	0.0	2.1694482701001965	0.0
10	4.306554317277638E-5	0.0	0.0	2.5364958945617695	0.0
11	4.306554317277638E-5	0.0	0.0	2.817584694850481	0.0
12	4.306554317277638E-5	0.0	0.0	3.0392861111039333	0.0
13	4.306554317277638E-5	0.0	0.0	3.1650590299400267	0.0
14	6.459831475916457E-5	0.0	0.0	3.253537188388496	0.0
15	6.459831475916457E-5	0.0	0.0	3.3191690761838073	0.0
16	6.459831475916457E-5	0.0	0.0	3.41430086105247	0.0
17	8.613108634555276E-5	0.0	0.0	3.528338419373982	0.0
18	1.0766385793194093E-4	0.0	0.0	3.6721342680278823	0.0
19	1.0766385793194093E-4	0.0	0.0	3.76950546114153	0.0
20	1.0766385793194093E-4	0.0	0.0	3.872367511009706	0.0
21	1.0766385793194093E-4	0.0	0.0	3.989742648927108	0.0
22	1.0766385793194093E-4	0.0	0.0	4.130523909558914	0.0
23	1.0766385793194093E-4	0.0	0.0	4.275030339675165	0.0
24	1.0766385793194093E-4	0.0	0.0	4.394321894263756	0.0
25	1.0766385793194093E-4	0.0	0.0	4.502437940399011	0.0
26	1.0766385793194093E-4	0.0	0.0	4.602716057676821	0.0
27	1.0766385793194093E-4	0.0	0.0	4.714169683407966	0.0
28	1.0766385793194093E-4	0.0	0.0	4.83087730540619	0.0
29	1.0766385793194093E-4	0.0	0.0	4.967244347862787	0.0
30	1.0766385793194093E-4	0.0	0.0	5.136427334217038	0.0
31	1.0766385793194093E-4	0.0	0.0	5.284379007787112	0.0
32	1.0766385793194093E-4	0.0	0.0	5.432610607387808	0.0
33	1.0766385793194093E-4	0.0	0.0	5.577913750052756	0.0
34	1.0766385793194093E-4	0.0	0.0	5.734887654917525	0.0
35	1.0766385793194093E-4	0.0	0.0	5.902821740519767	0.0
36	1.0766385793194093E-4	0.0	0.0	6.068494885105437	0.0
37	1.0766385793194093E-4	0.0	0.0	6.233823505345726	0.0
38	1.0766385793194093E-4	0.0	0.0	6.435391780165905	0.0
39	1.0766385793194093E-4	0.0	0.0	6.728969587974722	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	48030	0.0	43.917866	1
CGGTCTA	495	0.0	41.363636	31
TCCGCGT	55	6.184564E-11	40.909092	42
CGACGGT	515	0.0	39.32039	28
CACGACG	520	0.0	38.942307	26
AGGGCGA	2575	0.0	38.79612	6
GACCGAT	1330	0.0	38.57143	9
GGGCGAT	4905	0.0	38.394493	7
GTTTTTT	56000	0.0	38.330357	2
TAGGGAC	5325	0.0	37.774647	5
TACGGGA	1940	0.0	37.46134	4
TAACGGG	1160	0.0	37.241383	3
GGACCGA	1635	0.0	37.155964	8
TAAGGGA	6050	0.0	36.892563	4
ATAGGGA	7185	0.0	36.670143	4
TTAGGGA	6660	0.0	36.587837	4
ACGGGAC	1710	0.0	36.57895	5
CGTAAGG	815	0.0	36.441715	2
TATGGGA	4625	0.0	36.291893	4
TAGGGAT	7870	0.0	36.222996	5
>>END_MODULE