FastQCFastQC Report
Thu 26 May 2016
SRR1548126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548126_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2745834
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT518221.8872954446627146No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC48040.1749559514522728No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA40500.1474961705623865No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT270450.043.9850271
TAATGCG1100.042.9545441
TCGTTAG700.041.7857131
TCACGAC5000.040.9525
CGACGGT5000.040.9528
ATCCGTT556.184564E-1140.90909210
GGGCGAT25350.040.295867
CGGTCTA5100.040.1470631
CTCACGA5100.039.70588324
ATTAGCG1700.039.7058831
CGTTAGG3200.039.3752
ACGGGAT10550.039.241715
TACGGGA11550.039.1558464
TAATACG1150.039.1304321
TTACGGG8400.039.107143
TGCACGG2750.038.4545442
ACGGTCT5350.038.27102730
GTTTTTT320750.037.971162
ACGTAGG3200.037.968752
TAGGGAT32100.037.920565
Produced by FastQC (version 0.11.5)