FastQCFastQC Report
Thu 26 May 2016
SRR1548125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548125_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1633841
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52700.322552806546047No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20230.12381865799670838No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC351.2123564E-745.0000049
CATCGTA302.1660653E-645.00000432
GCGAACT253.8915307E-545.012
TACGCGT207.03416E-445.022
CGTACGA207.03416E-445.012
GACGCGT207.03416E-445.028
TCACGAC4450.042.47191225
GATTACG700.041.7857171
CACGACG4550.041.53846426
TATTGCG1100.040.9090921
TGTAGCG1100.040.9090921
CGTTTTT27600.040.4347841
CGTTAGG3250.040.1538432
CGCAATC451.9292202E-840.020
TATCCGG451.9292202E-840.02
CGGTCTA4650.039.6774231
CTCACGA4850.038.9690724
CGTAAGG1800.038.752
ACGGGTA2150.038.7209325
TCTCACG4950.038.63636423
Produced by FastQC (version 0.11.5)