##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548125_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1633841
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49121242519927	31.0	31.0	33.0	30.0	34.0
2	31.900604159156245	31.0	31.0	34.0	30.0	34.0
3	32.010865194348774	33.0	31.0	34.0	30.0	34.0
4	35.718897983341094	37.0	35.0	37.0	35.0	37.0
5	35.61046699158609	37.0	35.0	37.0	33.0	37.0
6	35.66867645015641	37.0	35.0	37.0	33.0	37.0
7	36.05145604743669	37.0	35.0	37.0	35.0	37.0
8	36.083490988413196	37.0	35.0	37.0	35.0	37.0
9	37.90588863910258	39.0	38.0	39.0	35.0	39.0
10	37.27064689893325	39.0	37.0	39.0	34.0	39.0
11	36.961049453404584	39.0	37.0	39.0	33.0	39.0
12	36.3892765575108	38.0	35.0	39.0	32.0	39.0
13	36.146710726441555	38.0	35.0	39.0	32.0	39.0
14	37.131415480453725	39.0	35.0	41.0	32.0	41.0
15	37.346911357959556	39.0	35.0	41.0	32.0	41.0
16	37.48098988824494	39.0	35.0	41.0	33.0	41.0
17	37.42139045353863	39.0	35.0	41.0	32.0	41.0
18	37.34537020432221	39.0	35.0	41.0	32.0	41.0
19	37.30491339120514	39.0	35.0	41.0	32.0	41.0
20	37.19072541330521	39.0	35.0	41.0	32.0	41.0
21	37.066028456869425	39.0	35.0	41.0	32.0	41.0
22	37.00689724397907	39.0	35.0	41.0	32.0	41.0
23	36.91372722315084	39.0	35.0	41.0	31.0	41.0
24	36.94790496749684	39.0	35.0	41.0	32.0	41.0
25	36.93950880165206	39.0	35.0	41.0	32.0	41.0
26	36.82545241550432	39.0	35.0	41.0	31.0	41.0
27	36.782776292185105	39.0	35.0	41.0	31.0	41.0
28	36.72886774172028	38.0	35.0	41.0	31.0	41.0
29	36.69747117375559	38.0	35.0	41.0	31.0	41.0
30	36.616913763334374	38.0	35.0	41.0	31.0	41.0
31	36.56183067997437	38.0	35.0	41.0	31.0	41.0
32	36.47622137037815	38.0	35.0	41.0	30.0	41.0
33	36.390564932573	38.0	35.0	41.0	30.0	41.0
34	36.273427463259885	38.0	35.0	41.0	30.0	41.0
35	36.19508140633024	38.0	35.0	41.0	30.0	41.0
36	36.05611072313646	38.0	35.0	41.0	30.0	41.0
37	35.99347366114573	38.0	35.0	41.0	29.0	41.0
38	35.887894844112736	38.0	35.0	40.0	29.0	41.0
39	35.83364660331085	38.0	35.0	40.0	29.0	41.0
40	35.692547806059466	38.0	35.0	40.0	28.0	41.0
41	35.612147693686225	38.0	35.0	40.0	28.0	41.0
42	35.57303556466021	38.0	35.0	40.0	28.0	41.0
43	35.48422949356761	38.0	34.0	40.0	27.0	41.0
44	35.32197013050842	38.0	34.0	40.0	27.0	41.0
45	35.30509517143957	38.0	34.0	40.0	27.0	41.0
46	35.22222603056234	38.0	34.0	40.0	27.0	41.0
47	35.13521817606487	38.0	34.0	40.0	27.0	41.0
48	35.00586164749201	37.0	34.0	40.0	26.0	41.0
49	34.92013849572878	37.0	34.0	40.0	26.0	41.0
50	34.749265687420014	37.0	34.0	40.0	26.0	41.0
51	34.392635513492436	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	3.0
13	10.0
14	14.0
15	41.0
16	128.0
17	295.0
18	611.0
19	1139.0
20	2089.0
21	3309.0
22	5039.0
23	7503.0
24	10496.0
25	14886.0
26	19648.0
27	23778.0
28	26968.0
29	31879.0
30	38022.0
31	46764.0
32	57785.0
33	74533.0
34	124635.0
35	184968.0
36	111797.0
37	148151.0
38	238726.0
39	460547.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.735498741921646	24.151187294234873	30.399347304909107	12.713966658934375
2	29.653619905486515	25.742345797418476	31.027560209347175	13.576474087747828
3	29.418223682720658	24.97990930574028	31.65240681314767	13.949460198391398
4	25.99273735938809	27.632248180820536	31.498413860345035	14.876600599446336
5	24.127868011636384	31.593771976587686	29.87402078904863	14.404339222727302
6	21.94448541810372	40.93409334200819	26.408750912726514	10.712670327161577
7	90.68373238277164	3.178644678398938	4.757929321151813	1.3796936176776076
8	91.74729976784766	2.3005298557203546	4.414383039720511	1.537787336711467
9	86.67483555621386	4.920368628281454	6.118832860725125	2.2859629547795657
10	42.509154807597554	32.60292770226723	13.908697357943645	10.979220132191566
11	34.81213900251004	24.648726528468803	24.248748807258476	16.29038566176268
12	34.52361643513659	21.998101406440405	25.82068879407482	17.657593364348184
13	25.507745245712403	29.27347275530483	26.317248740850545	18.901533258132215
14	18.345787625601268	33.05615417901742	28.22563517502621	20.3724230203551
15	17.168133251644438	28.323992359109607	37.099326066612356	17.4085483226336
16	23.130402529989148	26.01538338185907	34.390433340820806	16.463780747330983
17	23.557494272698506	25.561483644981365	25.942426466222845	24.93859561609728
18	23.88586159852764	27.856321392350907	29.916803409878927	18.34101359924252
19	26.96002854622941	28.184199074450937	25.9031325569624	18.952639822357256
20	28.65945951901072	28.213822520061623	26.11410779873929	17.012610162188366
21	26.02438058538132	27.050429019714894	29.25951790902542	17.66567248587837
22	24.261724366079687	23.644528445546413	29.533595986390353	22.560151201983548
23	21.274346769361276	27.2275576387176	29.715743453616355	21.782352138304766
24	21.272020961648042	26.104804567886347	33.19631469647291	19.426859773992696
25	20.46961730058188	28.644647796205387	30.77655659271618	20.109178310496553
26	19.918584488943537	33.51476673678773	26.1275730012896	20.439075772979134
27	19.65913451798553	32.797010235390104	29.05962085661946	18.484234390004904
28	16.647029912947463	29.903950261989998	34.01744723017723	19.431572594885303
29	19.002950715522502	27.14584834142368	33.07947346161591	20.771727481437914
30	20.20949406949636	29.055152857591406	30.519187607606863	20.216165465305373
31	24.894527680478088	27.69486137267947	27.422252226501843	19.988358720340596
32	25.976823938192272	26.69972169874547	28.104754379404117	19.21869998365814
33	24.58678659673738	27.718915120871614	27.80723460850842	19.887063673882587
34	19.808414649895553	28.51960502888592	29.471350027328242	22.200630293890285
35	20.44948070222255	27.261832699754752	29.544062121099913	22.744624476922784
36	24.666904551911724	26.65149179142891	28.57615887959722	20.10544477706215
37	20.332700672831688	30.29695056006062	29.32586463431876	20.04448413278893
38	20.42683467975158	30.275467441446263	26.855305993667685	22.442391885134477
39	20.7770523569919	29.096344136302125	28.854154106794972	21.272449399911007
40	22.754233735106418	27.401136340684314	28.00921264676306	21.835417277446215
41	19.540518324610535	26.155911132111388	28.56018425293526	25.74338629034282
42	21.43696969288933	26.125430809974777	27.71481435464038	24.722785142495507
43	21.38402696468016	26.263449136115447	28.5915214515978	23.76100244760659
44	20.31293130726919	28.381709113677523	29.22842553222743	22.076934046825855
45	19.476313790632013	31.040229740837695	26.416585212392146	23.066871256138143
46	21.586923084926866	29.539349300207302	27.570491865487522	21.303235749378306
47	20.756364909437334	27.62502593581628	28.862110817392878	22.756498337353513
48	20.90411490469391	27.02631406605661	30.918002424960566	21.15156860428891
49	21.35721897051182	25.198167997987564	30.897192566473723	22.54742046502689
50	20.29775235166702	29.01004442904787	28.762284702122177	21.929918517162932
51	19.230145405825905	29.999430789164922	27.720567668457335	23.049856136551846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	792.0
1	1717.5
2	2643.0
3	5918.0
4	9193.0
5	6879.0
6	4565.0
7	4332.0
8	4099.0
9	4296.0
10	4493.0
11	4468.0
12	4443.0
13	4381.5
14	4320.0
15	4354.0
16	4388.0
17	4265.5
18	4143.0
19	4189.0
20	4235.0
21	5237.0
22	6239.0
23	6608.5
24	6978.0
25	9574.5
26	15015.5
27	17860.0
28	20031.5
29	22203.0
30	26792.0
31	31381.0
32	36972.5
33	42564.0
34	48874.5
35	55185.0
36	58328.5
37	61472.0
38	69015.5
39	76559.0
40	92477.0
41	108395.0
42	124584.0
43	140773.0
44	143735.0
45	146697.0
46	146475.0
47	146253.0
48	144127.5
49	142002.0
50	136665.0
51	131328.0
52	118699.5
53	106071.0
54	95359.0
55	84647.0
56	77376.0
57	70105.0
58	63379.5
59	56654.0
60	49776.0
61	42898.0
62	36808.0
63	30718.0
64	24505.0
65	18292.0
66	15452.0
67	12612.0
68	10122.0
69	7632.0
70	5764.0
71	3896.0
72	3092.0
73	2288.0
74	1793.5
75	980.5
76	662.0
77	523.0
78	384.0
79	259.5
80	135.0
81	120.0
82	105.0
83	70.0
84	35.0
85	26.0
86	17.0
87	13.0
88	9.0
89	5.5
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1633841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.469643208119642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.83999379644091	17.356058237561914
2	7.399209274779035	3.1771676630343118
3	2.3001104363042666	1.4814765122017501
4	1.1219029804037262	0.9634742681357616
5	0.6650347507735221	0.7139029410054144
6	0.4413802875212136	0.568576637530663
7	0.3453599262373457	0.5190328076288832
8	0.2730409623857914	0.46896736348996443
9	0.2263868981369158	0.4374401336993257
>10	3.580445332174542	19.996292748668058
>50	1.975910887874453	30.764081065814963
>100	0.8280813012286862	22.752878688822996
>500	0.0025716810596710694	0.35324098297401874
>1k	2.8574233996345215E-4	0.12410672256971864
>5k	2.8574233996345215E-4	0.3233032268622923
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5270	0.322552806546047	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2023	0.12381865799670838	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.120546613776983E-5	0.0	0.0	0.05894086389067235	0.0
2	6.120546613776983E-5	0.0	0.0	0.20607880448587101	0.0
3	6.120546613776983E-5	0.0	0.0	0.29243971720626427	0.0
4	6.120546613776983E-5	0.0	0.0	0.44269913657448917	0.0
5	6.120546613776983E-5	0.0	0.0	0.7579072871840038	0.0
6	6.120546613776983E-5	0.0	0.0	1.0158883269547037	0.0
7	6.120546613776983E-5	0.0	0.0	1.18175514018806	0.0
8	6.120546613776983E-5	0.0	0.0	1.5246281614918464	0.0
9	6.120546613776983E-5	0.0	0.0	1.6311868780377037	0.0
10	6.120546613776983E-5	0.0	0.0	1.878701783098845	0.0
11	6.120546613776983E-5	0.0	0.0	2.2788631207075842	0.0
12	6.120546613776983E-5	0.0	0.0	2.5840947803366423	0.0
13	6.120546613776983E-5	0.0	0.0	2.6997119058708896	0.0
14	6.120546613776983E-5	0.0	0.0	2.752226195817096	0.0
15	6.120546613776983E-5	0.0	0.0	2.8406680943861735	0.0
16	6.120546613776983E-5	0.0	0.0	3.0377496953497922	0.0
17	6.120546613776983E-5	0.0	0.0	3.2706364940040067	0.0
18	6.120546613776983E-5	0.0	0.0	3.5232314527545827	0.0
19	6.120546613776983E-5	0.0	0.0	3.692280950227103	0.0
20	1.2241093227553966E-4	0.0	0.0	3.8724086370705595	0.0
21	1.2241093227553966E-4	0.0	0.0	4.109885845685106	0.0
22	1.2241093227553966E-4	0.0	0.0	4.368846172914011	0.0
23	1.2241093227553966E-4	0.0	0.0	4.613851653863503	0.0
24	1.2241093227553966E-4	0.0	0.0	4.796121532021782	0.0
25	1.2241093227553966E-4	0.0	0.0	4.9701898777175995	0.0
26	1.2241093227553966E-4	0.0	0.0	5.13923937519012	0.0
27	1.2241093227553966E-4	0.0	0.0	5.323039390001842	0.0
28	1.2241093227553966E-4	0.0	0.0	5.504391186168054	0.0
29	1.2241093227553966E-4	0.0	0.0	5.711816510908957	0.0
30	1.2241093227553966E-4	0.0	0.0	5.956699580926173	0.0
31	1.2241093227553966E-4	0.0	0.0	6.1681032609660305	0.0
32	1.2241093227553966E-4	0.0	0.0	6.356432480271948	0.0
33	1.2241093227553966E-4	0.0	0.0	6.555839888948802	0.0
34	1.2241093227553966E-4	0.0	0.0	6.756838639745238	0.0
35	1.2241093227553966E-4	0.0	0.0	6.989603027467177	0.0
36	1.2241093227553966E-4	0.0	0.0	7.202598049626616	0.0
37	1.2241093227553966E-4	0.0	0.0	7.427895370479747	0.0
38	1.2241093227553966E-4	0.0	0.0	7.690099587413953	0.0
39	1.2241093227553966E-4	0.0	0.0	8.067064053356477	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	35	1.2123564E-7	45.000004	9
CATCGTA	30	2.1660653E-6	45.000004	32
GCGAACT	25	3.8915307E-5	45.0	12
TACGCGT	20	7.03416E-4	45.0	22
CGTACGA	20	7.03416E-4	45.0	12
GACGCGT	20	7.03416E-4	45.0	28
TCACGAC	445	0.0	42.471912	25
GATTACG	70	0.0	41.785717	1
CACGACG	455	0.0	41.538464	26
TATTGCG	110	0.0	40.909092	1
TGTAGCG	110	0.0	40.909092	1
CGTTTTT	2760	0.0	40.434784	1
CGTTAGG	325	0.0	40.153843	2
CGCAATC	45	1.9292202E-8	40.0	20
TATCCGG	45	1.9292202E-8	40.0	2
CGGTCTA	465	0.0	39.67742	31
CTCACGA	485	0.0	38.96907	24
CGTAAGG	180	0.0	38.75	2
ACGGGTA	215	0.0	38.720932	5
TCTCACG	495	0.0	38.636364	23
>>END_MODULE