FastQCFastQC Report
Thu 26 May 2016
SRR1548124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548124_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2262138
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125290.5538565728527614No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTG207.034738E-445.035
TCGGTAC207.034738E-445.036
CGACGGT3350.042.98507728
CGTAAGG2850.042.631582
CGTTTTT59000.042.1779631
CGGTCTA3600.041.87500431
TAGCACG650.041.5384641
AAACGCG451.9297659E-840.0000041
CGGGCGA3450.039.7826086
TTTCGCG850.039.7058831
TATCGCG850.039.7058831
CCAATCG403.460209E-739.37524
GGGCGAT22400.038.8727657
TACGGGA11000.038.8636364
TAGGGAC32250.038.5813985
AGGGATC37300.038.243976
GTTACGG2650.038.2075462
AGGGCGA11450.038.1222726
TGGGCGA6150.038.048786
GACCGAT8200.037.8658569
Produced by FastQC (version 0.11.5)