##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548124_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2262138
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53486922548492	31.0	31.0	33.0	30.0	34.0
2	31.95085799363257	31.0	31.0	34.0	30.0	34.0
3	32.0124806709405	33.0	31.0	34.0	30.0	34.0
4	35.72888347218428	37.0	35.0	37.0	35.0	37.0
5	35.6358586434603	37.0	35.0	37.0	33.0	37.0
6	35.69960409135075	37.0	35.0	37.0	33.0	37.0
7	36.095429191322545	37.0	35.0	37.0	35.0	37.0
8	36.1339591130161	37.0	35.0	37.0	35.0	37.0
9	37.96011693362651	39.0	38.0	39.0	35.0	39.0
10	37.367181400957854	39.0	37.0	39.0	34.0	39.0
11	36.99618414084375	39.0	37.0	39.0	33.0	39.0
12	36.63693063818388	39.0	35.0	39.0	33.0	39.0
13	36.509124553851265	39.0	35.0	39.0	32.0	39.0
14	37.58867186705674	40.0	36.0	41.0	33.0	41.0
15	37.750283581284606	40.0	36.0	41.0	33.0	41.0
16	37.82717102139657	40.0	36.0	41.0	33.0	41.0
17	37.76222936001252	40.0	36.0	41.0	33.0	41.0
18	37.65954817964244	39.0	36.0	41.0	33.0	41.0
19	37.62162255353122	39.0	36.0	41.0	33.0	41.0
20	37.51471218820426	39.0	35.0	41.0	32.0	41.0
21	37.403325968619065	39.0	35.0	41.0	32.0	41.0
22	37.3350299583845	39.0	35.0	41.0	32.0	41.0
23	37.24035271057734	39.0	35.0	41.0	32.0	41.0
24	37.263837573127724	39.0	35.0	41.0	32.0	41.0
25	37.26235844143903	39.0	35.0	41.0	32.0	41.0
26	37.14545620116898	39.0	35.0	41.0	32.0	41.0
27	37.08948083627082	39.0	35.0	41.0	32.0	41.0
28	37.04459895903786	39.0	35.0	41.0	32.0	41.0
29	36.99137187916917	39.0	35.0	41.0	31.0	41.0
30	36.89765390086723	39.0	35.0	41.0	31.0	41.0
31	36.82809404200805	39.0	35.0	41.0	31.0	41.0
32	36.728238065051734	39.0	35.0	41.0	31.0	41.0
33	36.606249928165305	39.0	35.0	41.0	30.0	41.0
34	36.499347519912575	39.0	35.0	41.0	30.0	41.0
35	36.36019155330046	39.0	35.0	41.0	30.0	41.0
36	36.20581149337485	39.0	35.0	41.0	30.0	41.0
37	36.14855680776328	39.0	35.0	41.0	30.0	41.0
38	36.06042425351592	39.0	35.0	41.0	29.0	41.0
39	36.00403202633969	39.0	35.0	41.0	29.0	41.0
40	35.89337432110685	38.0	35.0	41.0	29.0	41.0
41	35.81250834387646	38.0	35.0	40.0	28.0	41.0
42	35.767194574336315	38.0	35.0	40.0	28.0	41.0
43	35.6685065190541	38.0	35.0	40.0	27.0	41.0
44	35.50665520847976	38.0	34.0	40.0	27.0	41.0
45	35.48363185623512	38.0	34.0	40.0	27.0	41.0
46	35.39015524251836	38.0	34.0	40.0	27.0	41.0
47	35.2934051768725	38.0	34.0	40.0	26.0	41.0
48	35.16547310553114	38.0	34.0	40.0	26.0	41.0
49	35.060955167191395	38.0	34.0	40.0	26.0	41.0
50	34.92564423567439	38.0	34.0	40.0	25.0	41.0
51	34.55222227821645	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	3.0
13	4.0
14	16.0
15	32.0
16	91.0
17	252.0
18	596.0
19	1211.0
20	2377.0
21	3860.0
22	6288.0
23	9605.0
24	13942.0
25	20447.0
26	27484.0
27	32573.0
28	36543.0
29	41289.0
30	49586.0
31	59680.0
32	75081.0
33	95778.0
34	153460.0
35	220979.0
36	157550.0
37	212408.0
38	346430.0
39	694463.0
40	108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.822390145959268	24.751628768890317	31.422220925513827	13.00376015963659
2	29.841415510459573	26.148360533265436	30.712670933426693	13.297553022848296
3	29.099329925937322	25.419846180913808	31.764994001250145	13.715829891898727
4	25.995628913885888	27.176547142570435	32.35890118109505	14.46892276244862
5	23.86127636775475	31.45099901067044	30.121018257948894	14.566706363625915
6	22.401993158684395	40.745745838671205	26.401439699965255	10.450821302679147
7	90.42273283062306	2.7476219399523814	5.447766670291556	1.3818785591329972
8	91.21786557672432	2.017295142913474	5.224703355851854	1.5401359245103525
9	86.00072144139747	5.266920055275142	6.570421433175164	2.161937070152219
10	40.17774335606404	39.78992439895356	11.741900803576085	8.290431441406316
11	28.357421165287	23.27364643536336	30.800950251487752	17.56798214786189
12	29.434366957276698	22.278835331885148	29.635062051917256	18.6517356589209
13	24.318896548309606	26.758447097391937	30.28714428562714	18.63551206867132
14	17.688266586742277	30.86235234101545	31.134528485883706	20.31485258635857
15	16.62820747452189	29.185266327695302	37.03156040878143	17.154965789001377
16	21.985705558193178	27.714047507269672	33.6989608945166	16.601286040020547
17	22.23807742940528	27.452215558909316	28.222150903260545	22.08755610842486
18	21.72882467824686	28.682732883670226	31.298842068874666	18.289600369208245
19	23.990799853943482	29.150476230893073	28.221620431644755	18.63710348351869
20	25.47223025297307	29.542406343025934	28.55462398845694	16.430739415544057
21	25.107752046957348	27.414552074188226	30.812709038971096	16.66498683988333
22	23.60359978038475	23.91962824549165	31.508599386951637	20.96817258717196
23	20.326390344002	27.565515454848466	31.861584041291913	20.24651015985762
24	19.99214902008631	27.012189353611493	34.75535091139444	18.240310714907757
25	20.09280600918246	29.718920773180063	31.104733663463502	19.083539554173974
26	19.70339563722461	32.93119164259652	27.77942813391579	19.585984586263084
27	18.720829586877546	31.450645362926576	31.508864622759532	18.319660427436347
28	17.17357650152201	29.893887994454804	34.08059101610954	18.851944487913645
29	18.291191784055616	27.599288814387098	33.88926758668127	20.22025181487602
30	20.047450686032416	28.131484462928434	32.880001131672785	18.94106371936637
31	22.818722818855434	27.669399479607343	29.998346696797455	19.513531004739765
32	23.953755252774144	27.805244419217573	29.61972258102733	18.62127774698095
33	22.59791400878284	27.94515630788219	29.466151048256116	19.99077863507885
34	20.032067009174508	27.76285089592235	31.173385531740326	21.031696563162814
35	19.88954696839892	27.57692059458795	31.15366967002013	21.379862766992996
36	22.418879838453712	27.44806019791896	30.773940405050443	19.359119558576886
37	20.009256729695537	29.19441696306768	31.304146785032565	19.492179522204214
38	19.444304458879166	29.781162776099425	29.27637482770724	21.49815793731417
39	19.87575470638838	28.709786936075517	30.822478557895227	20.591979799640868
40	22.367645121561992	26.99211984414744	30.08096765095675	20.55926738333382
41	19.230922251427632	26.217100813478222	31.11631562707492	23.435661308019228
42	20.368872279233187	27.022224108343522	29.659950011891407	22.948953600531887
43	20.39632418535032	27.520115925730437	29.909625319056577	22.17393456986267
44	20.087678116896495	28.85946834366427	29.583473687281675	21.469379852157562
45	19.80056035485015	30.23449497775998	28.279618661637794	21.68532600575208
46	21.478309457690024	29.396261412875784	28.807172683540973	20.31825644589322
47	20.288505829440997	27.609986658638864	30.635045253649423	21.466462258270717
48	20.181217945147466	27.00224301081543	31.797883241429126	21.018655802607974
49	20.627654015802747	26.570836969274197	31.492773650413902	21.30873536450915
50	19.497307414490187	28.242485648532494	31.135766252987217	21.1244406839901
51	18.455991632694378	29.237429369914658	30.009088747017206	22.297490250373762
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1024.0
1	1928.5
2	2833.0
3	11646.0
4	20459.0
5	14543.5
6	8628.0
7	8056.0
8	7484.0
9	7753.0
10	8022.0
11	8028.0
12	8034.0
13	7868.5
14	7703.0
15	7584.5
16	7466.0
17	7461.0
18	7456.0
19	8049.0
20	8642.0
21	9756.0
22	10870.0
23	12482.5
24	14095.0
25	17429.5
26	27349.0
27	33934.0
28	39658.0
29	45382.0
30	53716.5
31	62051.0
32	70287.5
33	78524.0
34	86078.0
35	93632.0
36	101132.0
37	108632.0
38	116678.0
39	124724.0
40	138814.0
41	152904.0
42	170722.0
43	188540.0
44	192342.5
45	196145.0
46	194181.5
47	192218.0
48	187143.5
49	182069.0
50	172027.0
51	161985.0
52	144605.0
53	127225.0
54	115708.0
55	104191.0
56	94758.0
57	85325.0
58	75336.5
59	65348.0
60	57502.0
61	49656.0
62	40105.5
63	30555.0
64	24727.0
65	18899.0
66	15407.5
67	11916.0
68	9075.5
69	6235.0
70	5070.5
71	3906.0
72	3038.5
73	2171.0
74	1591.5
75	845.5
76	679.0
77	541.5
78	404.0
79	336.0
80	268.0
81	163.0
82	58.0
83	41.0
84	24.0
85	23.5
86	23.0
87	15.0
88	7.0
89	5.5
90	4.0
91	3.0
92	2.0
93	4.5
94	7.0
95	5.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2262138.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.124576807712682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.31657468327663	17.54845648704833
2	7.832542065476177	3.465833570545364
3	2.519996145047577	1.6726154479873494
4	1.2096397009562487	1.0705106589386049
5	0.7213141674705769	0.7979385350347054
6	0.5097631416185934	0.6766976268288893
7	0.38984798589156905	0.6037655195033076
8	0.3375165490663552	0.5973928650954162
9	0.27499918122948164	0.5475816456452789
>10	4.34013984864567	24.537050931572757
>50	1.957138153769908	31.05023167194545
>100	0.587511375251312	16.384218623353462
>500	0.0022124683533034837	0.32467150929290495
>1k	6.034004599918592E-4	0.16549524987120526
>5k	0.0	0.0
>10k+	2.0113348666395307E-4	0.5575396573370074
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12529	0.5538565728527614	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.048140299132944146	0.0
2	0.0	0.0	0.0	0.16661229332604818	0.0
3	0.0	0.0	0.0	0.2722645568042268	0.0
4	0.0	0.0	0.0	0.4132373887004241	0.0
5	0.0	0.0	0.0	0.7691396369275437	0.0
6	0.0	0.0	0.0	1.111249623144123	0.0
7	0.0	0.0	0.0	1.345718077323311	0.0
8	0.0	0.0	0.0	1.800376458023339	0.0
9	0.0	0.0	0.0	1.9619050650313996	0.0
10	4.420596798250151E-5	0.0	0.0	2.2291743474536037	0.0
11	4.420596798250151E-5	0.0	0.0	2.6644263082093134	0.0
12	4.420596798250151E-5	0.0	0.0	2.998446602285095	0.0
13	4.420596798250151E-5	0.0	0.0	3.1293404734812817	0.0
14	4.420596798250151E-5	0.0	0.0	3.187250291538359	0.0
15	4.420596798250151E-5	0.0	0.0	3.2718605142568666	0.0
16	4.420596798250151E-5	0.0	0.0	3.4598242901184633	0.0
17	4.420596798250151E-5	0.0	0.0	3.710383716643282	0.0
18	4.420596798250151E-5	0.0	0.0	3.9730113724273233	0.0
19	4.420596798250151E-5	0.0	0.0	4.160046822961287	0.0
20	4.420596798250151E-5	0.0	0.0	4.355348789507978	0.0
21	4.420596798250151E-5	0.0	0.0	4.599542556643317	0.0
22	4.420596798250151E-5	0.0	0.0	4.895855160030025	0.0
23	4.420596798250151E-5	0.0	0.0	5.178861767053999	0.0
24	4.420596798250151E-5	0.0	0.0	5.394719508712554	0.0
25	4.420596798250151E-5	0.0	0.0	5.579721484719323	0.0
26	4.420596798250151E-5	0.0	0.0	5.762203720551089	0.0
27	4.420596798250151E-5	0.0	0.0	5.954057621595146	0.0
28	4.420596798250151E-5	0.0	0.0	6.152011946220788	0.0
29	4.420596798250151E-5	0.0	0.0	6.38002632907453	0.0
30	4.420596798250151E-5	0.0	0.0	6.654722214117795	0.0
31	8.841193596500302E-5	0.0	0.0	6.898606539477256	0.0
32	8.841193596500302E-5	0.0	0.0	7.105888323347205	0.0
33	8.841193596500302E-5	0.0	0.0	7.32280700823734	0.0
34	8.841193596500302E-5	0.0	0.0	7.560060438399426	0.0
35	8.841193596500302E-5	0.0	0.0	7.824058479190925	0.0
36	8.841193596500302E-5	0.0	0.0	8.070418338757406	0.0
37	8.841193596500302E-5	0.0	0.0	8.324691066592754	0.0
38	8.841193596500302E-5	0.0	0.0	8.597397683076807	0.0
39	8.841193596500302E-5	0.0	0.0	8.96134541747674	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTG	20	7.034738E-4	45.0	35
TCGGTAC	20	7.034738E-4	45.0	36
CGACGGT	335	0.0	42.985077	28
CGTAAGG	285	0.0	42.63158	2
CGTTTTT	5900	0.0	42.177963	1
CGGTCTA	360	0.0	41.875004	31
TAGCACG	65	0.0	41.538464	1
AAACGCG	45	1.9297659E-8	40.000004	1
CGGGCGA	345	0.0	39.782608	6
TTTCGCG	85	0.0	39.705883	1
TATCGCG	85	0.0	39.705883	1
CCAATCG	40	3.460209E-7	39.375	24
GGGCGAT	2240	0.0	38.872765	7
TACGGGA	1100	0.0	38.863636	4
TAGGGAC	3225	0.0	38.581398	5
AGGGATC	3730	0.0	38.24397	6
GTTACGG	265	0.0	38.207546	2
AGGGCGA	1145	0.0	38.122272	6
TGGGCGA	615	0.0	38.04878	6
GACCGAT	820	0.0	37.865856	9
>>END_MODULE