FastQCFastQC Report
Thu 26 May 2016
SRR1548123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548123_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1367108
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41320.30224386076301213No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23600.17262718088110082No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAT253.8911938E-545.00000441
ACCGTGC253.8911938E-545.00000425
GTACGTA253.8911938E-545.00000420
TATCCGT253.8911938E-545.00000418
GTATTCG406.8175723E-945.09
TCGATAT351.2121563E-745.036
CCGTACT207.0337567E-445.018
TGTTCGG950.045.02
CGGTATG207.0337567E-445.041
ACACGAT302.1658052E-644.99999626
ATCACGC302.1658052E-644.99999615
TCGGTTA302.1658052E-644.99999624
CGACGGT6250.043.5628
TTGCGAG1300.043.2692341
ACGGGTA1250.043.1999975
CGGGTAT1150.043.043486
CGGTCTA6200.042.82258231
CTCACGA6350.042.51968424
TCACGAC6400.042.187525
ACGGTCT6400.042.187530
Produced by FastQC (version 0.11.5)