##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548123_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1367108
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.512626654221904	31.0	31.0	33.0	30.0	34.0
2	31.916573526012577	31.0	31.0	34.0	30.0	34.0
3	32.01480351223166	33.0	31.0	34.0	30.0	34.0
4	35.73128677470982	37.0	35.0	37.0	35.0	37.0
5	35.61317320943188	37.0	35.0	37.0	33.0	37.0
6	35.67460434727907	37.0	35.0	37.0	33.0	37.0
7	36.059078726772135	37.0	35.0	37.0	35.0	37.0
8	36.093185030004946	37.0	35.0	37.0	35.0	37.0
9	37.96271691775632	39.0	38.0	39.0	35.0	39.0
10	37.3038728469148	39.0	37.0	39.0	34.0	39.0
11	36.93436144035438	39.0	37.0	39.0	33.0	39.0
12	36.16670592228266	38.0	35.0	39.0	32.0	39.0
13	35.7917728518888	38.0	35.0	39.0	31.0	39.0
14	36.74827665407561	39.0	35.0	41.0	31.0	41.0
15	37.021594489974454	39.0	35.0	41.0	32.0	41.0
16	37.19404465484804	39.0	35.0	41.0	32.0	41.0
17	37.14023764033273	39.0	35.0	41.0	32.0	41.0
18	37.08256699543855	38.0	35.0	41.0	32.0	41.0
19	37.05003847538015	38.0	35.0	41.0	32.0	41.0
20	36.92614701984042	38.0	35.0	41.0	32.0	41.0
21	36.76840015565705	38.0	35.0	41.0	32.0	41.0
22	36.69245224225153	38.0	35.0	41.0	31.0	41.0
23	36.645534954078244	38.0	35.0	40.0	31.0	41.0
24	36.662745006246766	38.0	35.0	41.0	31.0	41.0
25	36.63394698882605	38.0	35.0	40.0	31.0	41.0
26	36.51819826963195	38.0	35.0	40.0	31.0	41.0
27	36.47885390181317	38.0	35.0	40.0	31.0	41.0
28	36.482227446551406	38.0	35.0	40.0	31.0	41.0
29	36.442610240010296	38.0	35.0	40.0	31.0	41.0
30	36.367888272177474	38.0	35.0	40.0	31.0	41.0
31	36.264892020235415	38.0	35.0	40.0	30.0	41.0
32	36.14893702618959	38.0	35.0	40.0	30.0	41.0
33	36.05314064433827	38.0	35.0	40.0	30.0	41.0
34	35.948971844214206	38.0	35.0	40.0	30.0	41.0
35	35.85130509074631	38.0	35.0	40.0	30.0	41.0
36	35.68651562275987	38.0	35.0	40.0	29.0	41.0
37	35.61413436246441	38.0	34.0	40.0	29.0	41.0
38	35.519548565292574	38.0	34.0	40.0	29.0	41.0
39	35.47420028264043	38.0	34.0	40.0	29.0	41.0
40	35.33556090667307	37.0	34.0	40.0	27.0	41.0
41	35.30767942254745	37.0	34.0	40.0	28.0	41.0
42	35.27714562419355	37.0	34.0	40.0	27.0	41.0
43	35.173815821427425	37.0	34.0	40.0	27.0	41.0
44	35.008826661829204	37.0	34.0	40.0	27.0	41.0
45	34.97342346032647	37.0	34.0	40.0	27.0	41.0
46	34.890683837707044	37.0	34.0	40.0	27.0	41.0
47	34.81995131328322	36.0	34.0	40.0	26.0	41.0
48	34.71890370036603	36.0	34.0	40.0	26.0	41.0
49	34.64594018907065	36.0	34.0	40.0	26.0	41.0
50	34.470926949443644	36.0	34.0	40.0	25.0	41.0
51	34.091039625252726	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	4.0
12	1.0
13	4.0
14	20.0
15	27.0
16	90.0
17	225.0
18	457.0
19	1008.0
20	1702.0
21	2887.0
22	4470.0
23	6780.0
24	9567.0
25	13565.0
26	17912.0
27	21033.0
28	23758.0
29	27307.0
30	32878.0
31	40089.0
32	50429.0
33	66626.0
34	118452.0
35	193384.0
36	87077.0
37	114156.0
38	182756.0
39	350386.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.55894925638648	24.423308180480255	30.602264049365523	12.41547851376775
2	28.46885542327307	26.073068111663456	32.4544951825313	13.003581282532178
3	28.544489535574364	25.56652437115429	31.83281789002771	14.056168203243635
4	27.106929372075943	26.103789898091446	31.75513565863121	15.034145071201396
5	22.880928207573945	31.70407897547231	30.44580237991439	14.969190437039357
6	21.739979577326736	40.936634121078946	27.083449149591694	10.239937152002621
7	90.56373015153156	3.3488210148722706	4.736494848980476	1.3509539846156997
8	92.18971727178833	2.178540393297384	4.267109840627076	1.3646324942872106
9	88.73775883105066	3.9126389429364763	5.314869052042706	2.034733173970162
10	48.23517966393291	26.870956793464746	12.995461953261922	11.898401589340418
11	40.63651152652168	23.69878605055343	21.206663994358895	14.458038428565995
12	39.313572885243886	21.978439157696393	22.595288740904156	16.112699216155562
13	24.080760261808138	34.59141486992981	23.76132683006756	17.566498038194496
14	16.904882423334513	37.52593065068744	26.946883494208212	18.622303431769836
15	16.261480438999698	27.27165666501842	40.97042808614974	15.496434809832142
16	20.595007855999672	23.2419823452134	38.78208598003962	17.380923818747313
17	20.646649716042916	24.06408272060437	25.739151552035395	29.550116011317325
18	22.825994727556274	27.246128323438967	30.71586151203855	19.212015436966208
19	27.68281657337972	28.143935958241777	24.393683600710407	19.7795638676681
20	30.421298097882538	26.001164501999842	25.085070089561324	18.4924673105563
21	24.339627886019247	28.831957680007726	27.629419182683446	19.19899525128958
22	24.322877197704937	23.15032901570322	27.613985142358903	24.912808644232936
23	20.768951684870544	28.97693525310363	27.604988047762134	22.649125014263685
24	21.420692439807244	25.40574702218113	34.65212697168036	18.521433566331265
25	19.614543986283454	27.2371312288422	32.522887730888854	20.625437053985493
26	17.72727538716766	35.50502228060987	25.61706902453939	21.15063330768308
27	17.544041875257843	34.90989738923333	29.759024159027668	17.787036576481157
28	15.872337810911793	29.901148994812406	35.78429794866243	18.442215245613365
29	17.82324439619986	27.139479836267512	35.3321025112866	19.70517325624603
30	19.577458401238236	30.846941134131317	30.243916354816154	19.331684109814294
31	26.71164238670244	27.86188069998859	25.846458363201734	19.580018550107233
32	27.694593258177115	27.92164188930209	26.509463773162032	17.874301079358762
33	27.018348221208566	28.188921431225623	25.12581303013368	19.666917317432127
34	20.030824192382752	28.7617364538866	28.037141176849232	23.170298176881417
35	19.960749260482714	27.884410010035783	29.62655474183459	22.528285987646914
36	26.55145021461362	26.62869356334686	27.58765218256348	19.232204039476034
37	19.754401261641362	32.42135954145539	28.586914859689212	19.23732433721403
38	20.657402341292713	32.38851648882166	24.909078141595252	22.045003028290378
39	20.10821383533708	30.577832914444215	28.21957007054307	21.094383179675635
40	24.01997501294704	27.176492274202186	26.761162980539943	22.042369732310835
41	18.151089745652868	25.533388730078382	28.872408032137916	27.443113492130834
42	21.472334299850488	26.6492479014094	26.146580957759006	25.73183684098111
43	21.87691096826293	26.72502830793178	26.77630443242231	24.62175629138298
44	19.945168925937086	29.788795033018605	28.82083931920521	21.445196721839093
45	19.33417111157275	34.96212442616092	24.37013023111561	21.33357423115072
46	22.559300362517078	31.798950777846375	25.91872770841806	19.723021151218482
47	21.17974585767913	29.267109840627075	25.77236034022184	23.780783961471954
48	21.472846329624286	27.739871319603132	29.683975223610716	21.103307127161862
49	20.9573786416289	25.97717224974179	30.014892751706522	23.050556356922787
50	19.435991889448385	31.333515713462283	28.19791852582239	21.032573871266937
51	18.8831460279656	32.88745292983437	25.84594633342794	22.383454708772092
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	578.0
1	1216.0
2	1854.0
3	4509.0
4	7164.0
5	5423.5
6	3683.0
7	3675.0
8	3667.0
9	3904.0
10	4141.0
11	4132.5
12	4124.0
13	4087.5
14	4051.0
15	3937.5
16	3824.0
17	3489.0
18	3154.0
19	3087.5
20	3021.0
21	3448.0
22	3875.0
23	4570.5
24	5266.0
25	6999.5
26	10115.0
27	11497.0
28	13083.0
29	14669.0
30	17691.0
31	20713.0
32	26106.5
33	31500.0
34	33975.5
35	36451.0
36	42339.0
37	48227.0
38	55548.5
39	62870.0
40	81114.0
41	99358.0
42	118224.0
43	137090.0
44	136237.5
45	135385.0
46	134632.0
47	133879.0
48	129942.5
49	126006.0
50	116079.0
51	106152.0
52	95934.0
53	85716.0
54	76413.0
55	67110.0
56	61224.5
57	55339.0
58	51360.0
59	47381.0
60	41870.0
61	36359.0
62	29286.0
63	22213.0
64	17626.0
65	13039.0
66	10761.0
67	8483.0
68	6596.0
69	4709.0
70	3675.0
71	2641.0
72	2133.0
73	1625.0
74	1150.5
75	534.0
76	392.0
77	266.5
78	141.0
79	133.5
80	126.0
81	109.0
82	92.0
83	75.0
84	58.0
85	49.0
86	40.0
87	27.5
88	15.0
89	15.5
90	16.0
91	8.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1367108.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.642647915169135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.39527991302145	16.802141052011404
2	7.893193118321013	3.2587281293587322
3	2.3851248495368087	1.4770587750812731
4	1.0461875250956436	0.8638432293516617
5	0.6083367948234334	0.627884113469131
6	0.42838776701308867	0.5305834707370018
7	0.2992364333228186	0.43239226355317495
8	0.2500064914900304	0.4128636784268341
9	0.1814130514890802	0.3370361174194979
>10	2.711295385381773	14.264493548776947
>50	1.547807500125656	23.495894952356558
>100	1.2516001825543024	36.786874528777034
>500	0.0010654939125036623	0.1412054327398738
>1k	0.0010654939125036623	0.5690007079408933
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4132	0.30224386076301213	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2360	0.17262718088110082	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.314711054283934E-5	0.0	0.0	0.045790091199817425	0.0
2	7.314711054283934E-5	0.0	0.0	0.16853094269070182	0.0
3	7.314711054283934E-5	0.0	0.0	0.2443113492130834	0.0
4	7.314711054283934E-5	0.0	0.0	0.3659549940458252	0.0
5	7.314711054283934E-5	0.0	0.0	0.6340391541853314	0.0
6	7.314711054283934E-5	0.0	0.0	0.8633553457371327	0.0
7	7.314711054283934E-5	0.0	0.0	1.000213589562785	0.0
8	7.314711054283934E-5	0.0	0.0	1.3152581946707942	0.0
9	1.4629422108567867E-4	0.0	0.0	1.3987921949107167	0.0
10	1.4629422108567867E-4	0.0	0.0	1.6197696158606343	0.0
11	1.4629422108567867E-4	0.0	0.0	1.969485951365949	0.0
12	1.4629422108567867E-4	0.0	0.0	2.2264517507029438	0.0
13	1.4629422108567867E-4	0.0	0.0	2.3328076494322323	0.0
14	1.4629422108567867E-4	0.0	0.0	2.3723070891253655	0.0
15	1.4629422108567867E-4	0.0	0.0	2.4472828774317756	0.0
16	1.4629422108567867E-4	0.0	0.0	2.6212998534131904	0.0
17	1.4629422108567867E-4	0.0	0.0	2.831817237555482	0.0
18	1.4629422108567867E-4	0.0	0.0	3.0898070964400763	0.0
19	1.4629422108567867E-4	0.0	0.0	3.2390272019474686	0.0
20	1.4629422108567867E-4	0.0	0.0	3.3912463389871172	0.0
21	1.4629422108567867E-4	0.0	0.0	3.6123700541581205	0.0
22	1.4629422108567867E-4	0.0	0.0	3.8417593928204647	0.0
23	1.4629422108567867E-4	0.0	0.0	4.086655918917891	0.0
24	1.4629422108567867E-4	0.0	0.0	4.25072488786548	0.0
25	1.4629422108567867E-4	0.0	0.0	4.404114378673814	0.0
26	1.4629422108567867E-4	0.0	0.0	4.554139102397177	0.0
27	1.4629422108567867E-4	0.0	0.0	4.713526656270024	0.0
28	1.4629422108567867E-4	0.0	0.0	4.875181770569699	0.0
29	1.4629422108567867E-4	0.0	0.0	5.057464370042455	0.0
30	1.4629422108567867E-4	0.0	0.0	5.285537060715027	0.0
31	1.4629422108567867E-4	0.0	0.0	5.49839515239469	0.0
32	1.4629422108567867E-4	0.0	0.0	5.68492028427893	0.0
33	1.4629422108567867E-4	0.0	0.0	5.8716648574948	0.0
34	1.4629422108567867E-4	0.0	0.0	6.067187083975809	0.0
35	1.4629422108567867E-4	0.0	0.0	6.31376599361572	0.0
36	1.4629422108567867E-4	0.0	0.0	6.51031227964433	0.0
37	1.4629422108567867E-4	0.0	0.0	6.742554355617845	0.0
38	1.4629422108567867E-4	0.0	0.0	6.972163135611817	0.0
39	1.4629422108567867E-4	0.0	0.0	7.285891092730055	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAAT	25	3.8911938E-5	45.000004	41
ACCGTGC	25	3.8911938E-5	45.000004	25
GTACGTA	25	3.8911938E-5	45.000004	20
TATCCGT	25	3.8911938E-5	45.000004	18
GTATTCG	40	6.8175723E-9	45.0	9
TCGATAT	35	1.2121563E-7	45.0	36
CCGTACT	20	7.0337567E-4	45.0	18
TGTTCGG	95	0.0	45.0	2
CGGTATG	20	7.0337567E-4	45.0	41
ACACGAT	30	2.1658052E-6	44.999996	26
ATCACGC	30	2.1658052E-6	44.999996	15
TCGGTTA	30	2.1658052E-6	44.999996	24
CGACGGT	625	0.0	43.56	28
TTGCGAG	130	0.0	43.269234	1
ACGGGTA	125	0.0	43.199997	5
CGGGTAT	115	0.0	43.04348	6
CGGTCTA	620	0.0	42.822582	31
CTCACGA	635	0.0	42.519684	24
TCACGAC	640	0.0	42.1875	25
ACGGTCT	640	0.0	42.1875	30
>>END_MODULE