FastQCFastQC Report
Thu 26 May 2016
SRR1548122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548122_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1604407
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57760.360008401858132No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGT207.0341234E-445.041
ACGCATT253.8914994E-545.036
ACTTACG253.8914994E-545.01
CGCTACG207.0341234E-445.012
AACGCGT207.0341234E-445.013
ACTCGAT207.0341234E-445.010
ACGTAAC207.0341234E-445.031
CGACGGT6150.042.43902628
TCTAGCG800.042.18751
CGTAAGG2400.042.1874962
TAGTGCG750.042.01
CGTTTTT28350.041.269841
GATCGTA603.6379788E-1241.2499969
TCGCGAG603.6379788E-1241.2499961
GTAGGGT9900.040.909094
TATTGCG2050.040.6097561
AGGGATT23050.040.3145336
CACGACG6650.039.9248126
AGTACGG2200.039.8863642
GGCGATA3750.039.6000028
Produced by FastQC (version 0.11.5)