##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1548122_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1604407 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.51965430218143 31.0 31.0 33.0 30.0 34.0 2 31.929051668311097 31.0 31.0 34.0 30.0 34.0 3 32.02065934641272 33.0 31.0 34.0 30.0 34.0 4 35.743455993398186 37.0 35.0 37.0 35.0 37.0 5 35.63273907431219 37.0 35.0 37.0 33.0 37.0 6 35.69363696368814 37.0 35.0 37.0 33.0 37.0 7 36.074665592957395 37.0 35.0 37.0 35.0 37.0 8 36.097090077517734 37.0 35.0 37.0 35.0 37.0 9 37.93883098241282 39.0 38.0 39.0 35.0 39.0 10 37.32269305731027 39.0 37.0 39.0 34.0 39.0 11 36.989102515758155 39.0 37.0 39.0 33.0 39.0 12 36.396486677009015 38.0 35.0 39.0 32.0 39.0 13 36.15181060666028 38.0 35.0 39.0 32.0 39.0 14 37.15121848757828 39.0 35.0 41.0 32.0 41.0 15 37.37466989361178 39.0 35.0 41.0 32.0 41.0 16 37.49648686399399 39.0 35.0 41.0 33.0 41.0 17 37.43940970090507 39.0 35.0 41.0 33.0 41.0 18 37.370434060684104 39.0 35.0 41.0 32.0 41.0 19 37.331736273900574 39.0 35.0 41.0 32.0 41.0 20 37.212083342942286 39.0 35.0 41.0 32.0 41.0 21 37.08638830421458 39.0 35.0 41.0 32.0 41.0 22 37.0224182517279 39.0 35.0 41.0 32.0 41.0 23 36.92905852442678 39.0 35.0 41.0 32.0 41.0 24 36.94296210375547 39.0 35.0 41.0 32.0 41.0 25 36.9131672948323 39.0 35.0 41.0 32.0 41.0 26 36.80262053207198 38.0 35.0 41.0 31.0 41.0 27 36.754554174844664 38.0 35.0 41.0 31.0 41.0 28 36.71485414860444 38.0 35.0 41.0 31.0 41.0 29 36.6572621535558 38.0 35.0 41.0 31.0 41.0 30 36.58199259913476 38.0 35.0 40.0 31.0 41.0 31 36.499454938802934 38.0 35.0 41.0 31.0 41.0 32 36.39567516222505 38.0 35.0 41.0 30.0 41.0 33 36.3060563809557 38.0 35.0 41.0 30.0 41.0 34 36.20594711940299 38.0 35.0 41.0 30.0 41.0 35 36.088349776584124 38.0 35.0 41.0 30.0 41.0 36 35.932291494614525 38.0 35.0 41.0 29.0 41.0 37 35.86010282926963 38.0 35.0 40.0 29.0 41.0 38 35.744357884252565 38.0 35.0 40.0 29.0 41.0 39 35.710490542611694 38.0 35.0 40.0 29.0 41.0 40 35.55015342117056 38.0 35.0 40.0 27.0 41.0 41 35.48477848825142 38.0 34.0 40.0 27.0 41.0 42 35.44135870760973 38.0 34.0 40.0 27.0 41.0 43 35.356332900567004 38.0 34.0 40.0 27.0 41.0 44 35.18784198772506 38.0 34.0 40.0 27.0 41.0 45 35.168906019482584 38.0 34.0 40.0 27.0 41.0 46 35.113667541964105 37.0 34.0 40.0 27.0 41.0 47 35.02656620171814 37.0 34.0 40.0 26.0 41.0 48 34.910830605949734 37.0 34.0 40.0 26.0 41.0 49 34.8277076826516 37.0 34.0 40.0 26.0 41.0 50 34.65442060524543 37.0 34.0 40.0 25.0 41.0 51 34.281797573807644 36.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 4.0 13 7.0 14 17.0 15 44.0 16 109.0 17 240.0 18 599.0 19 1106.0 20 2045.0 21 3292.0 22 4975.0 23 7267.0 24 10730.0 25 15133.0 26 19935.0 27 23874.0 28 26863.0 29 30970.0 30 37430.0 31 45662.0 32 56852.0 33 73465.0 34 124282.0 35 191069.0 36 109783.0 37 142713.0 38 228796.0 39 447062.0 40 81.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.22891697680202 24.4727802858003 30.713466096819573 12.584836640578107 2 28.963411403714893 25.519896136080185 31.461717631498743 14.054974828706182 3 29.110568577673867 25.33291116281592 31.836373189595907 13.720147069914304 4 26.642740900532097 27.535656476193388 31.356507419875378 14.465095203399137 5 23.828866366202593 32.21981704143649 29.87670834146199 14.07460825089893 6 21.169877718060317 41.40514221142141 27.30610125734929 10.118878813168978 7 90.55850541664303 3.1831698565264297 4.859801783462675 1.3985229433678612 8 91.31504661847025 2.45455174404001 4.547599206435773 1.6828024310539658 9 87.05908164200231 4.668578484137753 6.075204109680399 2.197135764179538 10 43.57167476831004 32.86890421196118 12.644734160347094 10.91468685938169 11 35.64450915509593 24.32693200665417 24.517407366086037 15.511151472163858 12 34.7488511331601 22.128487347661785 25.946845158366923 17.17581636081119 13 24.71710731753227 30.550103558511026 26.198651589029463 18.534137534927233 14 17.930051414634814 33.73620284628526 28.26321500716464 20.07053073191528 15 16.488584255740594 28.871103155246768 37.736871005923064 16.903441583089577 16 21.411462303517748 25.986361316050104 36.072393102248995 16.529783278183157 17 21.3452696229822 25.96647858055967 27.02687036394132 25.661381432516812 18 22.97702515633502 27.631766752451213 30.829272123594574 18.56193596761919 19 26.27070313206063 28.918410353482628 25.474147145954863 19.33673936850188 20 28.31058453372492 27.84767206824702 25.947842411557666 17.893900986470392 21 24.923788041313706 28.26464855862633 29.6565023712811 17.155061028778857 22 24.523328556906073 22.58903133681167 29.537766913258295 23.349873193023964 23 21.083428332087806 28.402705797219785 29.03078832241445 21.48307754827796 24 21.197426837454586 26.059223127298747 34.18565239368814 18.557697641558534 25 20.278707335482828 28.19882984803731 31.229232981406835 20.29322983507302 26 19.427551737183894 33.42319000104088 26.390622828247444 20.75863543352778 27 18.39620495298263 33.24711248455037 29.72294436511434 18.633738197352667 28 17.038694047084064 29.340186124842386 34.686585137063105 18.934534691010448 29 17.98259419212207 27.065451596758177 34.18864415326036 20.763310057859382 30 19.806694934639403 30.02025047260452 30.359877512376848 19.81317708037923 31 25.155961049783503 27.65501521746041 26.902276043422894 20.286747689333193 32 25.536724783673968 28.011657889799785 27.6238510552497 18.827766271276552 33 24.705327264216624 27.60434228970579 27.485793816656244 20.204536629421337 34 19.158854330603145 28.198954504686156 29.483354286038395 23.158836878672307 35 20.008202407493858 27.45930427877714 30.434235203411603 22.098258110317396 36 25.1620692255768 26.46959281528939 28.695087967080674 19.673249992053137 37 19.966941056726878 30.45330767068456 28.835139712055607 20.74461156053296 38 20.496607157660122 30.797235364841963 27.2776172130887 21.428540264409218 39 20.296533236267354 29.042194405783572 28.893354367065214 21.76791799088386 40 23.238928775553834 27.064392015243016 27.95880347069042 21.737875738512734 41 19.45672139301312 25.902654376352135 29.58401452997899 25.05660970065576 42 21.317969816885615 26.213174088619656 27.8493549330064 24.619501161488326 43 21.241742276118217 26.78273031718261 27.679073950687076 24.296453456012095 44 20.283755929760964 28.30802907242364 28.880764045532086 22.527450952283303 45 18.899630829334452 32.332631308639264 26.558597662563177 22.209140199463103 46 21.61483962610485 30.34890772727868 27.153209877543542 20.883042769072937 47 20.770664800141112 27.951074758462163 28.409873554528247 22.868386886868482 48 20.578506575949866 26.827232740819507 30.816183175466076 21.77807750776455 49 21.367832476422752 25.682635391144515 30.834009076250602 22.11552305618213 50 20.18976481653346 29.375152314842808 29.289263883790085 21.145818984833646 51 18.632990257459607 30.44283651218176 27.745765257818 23.178407972540633 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 978.0 1 1854.0 2 2730.0 3 6357.0 4 9984.0 5 7418.0 6 4852.0 7 4762.0 8 4672.0 9 4840.0 10 5008.0 11 5043.0 12 5078.0 13 5057.5 14 5037.0 15 4645.0 16 4253.0 17 4243.5 18 4234.0 19 4486.0 20 4738.0 21 5774.5 22 6811.0 23 7813.0 24 8815.0 25 10754.0 26 14425.5 27 16158.0 28 21507.0 29 26856.0 30 29113.0 31 31370.0 32 38540.5 33 45711.0 34 50496.0 35 55281.0 36 61729.0 37 68177.0 38 72196.5 39 76216.0 40 90292.5 41 104369.0 42 121034.0 43 137699.0 44 141415.5 45 145132.0 46 142756.5 47 140381.0 48 139874.0 49 139367.0 50 129633.5 51 119900.0 52 109589.0 53 99278.0 54 89628.5 55 79979.0 56 73011.5 57 66044.0 58 58991.0 59 51938.0 60 46147.0 61 40356.0 62 35421.5 63 30487.0 64 25116.0 65 19745.0 66 15810.0 67 11875.0 68 9600.5 69 7326.0 70 5732.5 71 4139.0 72 3602.5 73 3066.0 74 2411.0 75 1290.5 76 825.0 77 686.0 78 547.0 79 420.0 80 293.0 81 194.5 82 96.0 83 74.0 84 52.0 85 57.5 86 63.0 87 40.5 88 18.0 89 15.0 90 12.0 91 9.0 92 6.0 93 3.5 94 1.0 95 2.5 96 4.0 97 2.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1604407.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.456979114728 #Duplication Level Percentage of deduplicated Percentage of total 1 80.38399785329635 17.247977630965202 2 7.827622271821974 3.3591425520892773 3 2.481836512239538 1.5975814262787942 4 1.1598655983042125 0.9954884767481995 5 0.6842667586528418 0.7341148774658327 6 0.5024323719620722 0.6468408547052061 7 0.3546495592386156 0.5326795729941326 8 0.2940496725335786 0.5047534145796484 9 0.22873554730039816 0.44171764750984693 >10 3.3380211721936415 18.421859705513402 >50 1.8052555773430532 28.02139941633867 >100 0.9349051835729457 26.460362615256493 >500 0.003198742463209727 0.4363960358945765 >1k 8.723843081481073E-4 0.23928778919869897 >5k 2.9079476938270244E-4 0.3603979844619779 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5776 0.360008401858132 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.232832442142175E-5 0.0 0.0 0.044190782014788016 0.0 2 6.232832442142175E-5 0.0 0.0 0.16523238804118906 0.0 3 6.232832442142175E-5 0.0 0.0 0.23940309410268093 0.0 4 6.232832442142175E-5 0.0 0.0 0.3950992485073925 0.0 5 6.232832442142175E-5 0.0 0.0 0.6785061396515971 0.0 6 6.232832442142175E-5 0.0 0.0 0.9505069474266816 0.0 7 6.232832442142175E-5 0.0 0.0 1.105143520316229 0.0 8 6.232832442142175E-5 0.0 0.0 1.4765580055434813 0.0 9 6.232832442142175E-5 0.0 0.0 1.5876894079868762 0.0 10 6.232832442142175E-5 0.0 0.0 1.8214206245672078 0.0 11 6.232832442142175E-5 0.0 0.0 2.2269287032529776 0.0 12 6.232832442142175E-5 0.0 0.0 2.5259176755025377 0.0 13 6.232832442142175E-5 0.0 0.0 2.653815397215295 0.0 14 6.232832442142175E-5 0.0 0.0 2.7110327990341605 0.0 15 6.232832442142175E-5 0.0 0.0 2.804213644044186 0.0 16 6.232832442142175E-5 0.0 0.0 3.009523144688349 0.0 17 6.232832442142175E-5 0.0 0.0 3.2401379450476093 0.0 18 6.232832442142175E-5 0.0 0.0 3.5076511134643518 0.0 19 6.232832442142175E-5 0.0 0.0 3.6822950784931754 0.0 20 6.232832442142175E-5 0.0 0.0 3.8658519939142626 0.0 21 6.232832442142175E-5 0.0 0.0 4.111986547054457 0.0 22 6.232832442142175E-5 0.0 0.0 4.374264136219799 0.0 23 6.232832442142175E-5 0.0 0.0 4.652934074707976 0.0 24 6.232832442142175E-5 0.0 0.0 4.848582685066819 0.0 25 6.232832442142175E-5 0.0 0.0 5.029397154213363 0.0 26 6.232832442142175E-5 0.0 0.0 5.203043866051445 0.0 27 6.232832442142175E-5 0.0 0.0 5.3925843006169885 0.0 28 6.232832442142175E-5 0.0 0.0 5.582561033453482 0.0 29 6.232832442142175E-5 0.0 0.0 5.797656081031808 0.0 30 6.232832442142175E-5 0.0 0.0 6.0565056123539724 0.0 31 6.232832442142175E-5 0.0 0.0 6.28786835260629 0.0 32 6.232832442142175E-5 0.0 0.0 6.492118271735289 0.0 33 6.232832442142175E-5 0.0 0.0 6.717310507869886 0.0 34 6.232832442142175E-5 0.0 0.0 6.964629299174088 0.0 35 6.232832442142175E-5 0.0 0.0 7.234697928892108 0.0 36 6.232832442142175E-5 0.0 0.0 7.461822343083768 0.0 37 6.232832442142175E-5 0.0 0.0 7.708393194494913 0.0 38 6.232832442142175E-5 0.0 0.0 7.9701721570648845 0.0 39 1.246566488428435E-4 0.0 0.0 8.39300750993981 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGCGT 20 7.0341234E-4 45.0 41 ACGCATT 25 3.8914994E-5 45.0 36 ACTTACG 25 3.8914994E-5 45.0 1 CGCTACG 20 7.0341234E-4 45.0 12 AACGCGT 20 7.0341234E-4 45.0 13 ACTCGAT 20 7.0341234E-4 45.0 10 ACGTAAC 20 7.0341234E-4 45.0 31 CGACGGT 615 0.0 42.439026 28 TCTAGCG 80 0.0 42.1875 1 CGTAAGG 240 0.0 42.187496 2 TAGTGCG 75 0.0 42.0 1 CGTTTTT 2835 0.0 41.26984 1 GATCGTA 60 3.6379788E-12 41.249996 9 TCGCGAG 60 3.6379788E-12 41.249996 1 GTAGGGT 990 0.0 40.90909 4 TATTGCG 205 0.0 40.609756 1 AGGGATT 2305 0.0 40.314533 6 CACGACG 665 0.0 39.92481 26 AGTACGG 220 0.0 39.886364 2 GGCGATA 375 0.0 39.600002 8 >>END_MODULE