FastQCFastQC Report
Thu 26 May 2016
SRR1548121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548121_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1004101
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37380.3722733071673069No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGCG207.032859E-445.0000041
GTCCGCC207.032859E-445.00000440
TCCGCGA253.890453E-545.037
CGACGGT2050.045.028
GCGCGTC253.890453E-545.038
TAACGTG351.2117198E-745.01
TAACGCG351.2117198E-745.01
CTACCGT253.890453E-545.027
CTATTCG253.890453E-545.015
CGGTCTA2000.045.031
TATCGCG253.890453E-545.01
GACGGTC2150.042.90697529
TAGCGAG950.042.631581
CTCACGA2200.041.9318224
ACGGTCT2200.041.9318230
GCGCGAC650.041.538469
CGCGACC650.041.5384610
TACGTCA556.002665E-1140.90909226
CGTTTTT23900.040.5753141
ACGACGG2350.040.21276527
Produced by FastQC (version 0.11.5)