FastQCFastQC Report
Thu 26 May 2016
SRR1548120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548120_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences662692
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36620.5525945688193007No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21310.32156718354831504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACC302.1641044E-645.0000046
TACCCTA302.1641044E-645.00000411
GATACCA1250.045.0000049
GGTACCG302.1641044E-645.0000041
CACGGGT302.1641044E-645.0000044
GCGCGAG850.045.0000041
GCGCGAC302.1641044E-645.0000049
TTTCGCG302.1641044E-645.0000042
TTCACGC302.1641044E-645.00000440
TAACGTG302.1641044E-645.00000415
CGCGACC302.1641044E-645.00000410
ATGTCGC302.1641044E-645.00000433
TCTCGCG2150.045.00000432
AACGACG302.1641044E-645.0000041
CTCGCGC2150.045.00000433
ACGCTCG302.1641044E-645.0000041
ACTCGCG302.1641044E-645.0000041
GTTCACG302.1641044E-645.00000439
CATCGGT302.1641044E-645.00000437
GGCGCGA302.1641044E-645.0000048
Produced by FastQC (version 0.11.5)