##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548120_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	662692
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.523564491498313	31.0	31.0	33.0	30.0	34.0
2	31.911029558226144	31.0	31.0	34.0	30.0	34.0
3	31.93265197105141	33.0	31.0	34.0	30.0	34.0
4	35.65780332341419	37.0	35.0	37.0	33.0	37.0
5	35.56393769654681	37.0	35.0	37.0	33.0	37.0
6	35.636503835869455	37.0	35.0	37.0	33.0	37.0
7	36.04291284639018	37.0	35.0	37.0	35.0	37.0
8	36.07951054185051	37.0	35.0	37.0	35.0	37.0
9	37.93471326045886	39.0	38.0	39.0	35.0	39.0
10	37.353001092513566	39.0	37.0	39.0	34.0	39.0
11	36.94211187097475	39.0	35.0	39.0	33.0	39.0
12	35.687805194570025	37.0	35.0	39.0	32.0	39.0
13	35.06372492802086	37.0	35.0	39.0	30.0	39.0
14	35.9315504035057	37.0	35.0	40.0	30.0	41.0
15	36.35817845997839	37.0	35.0	40.0	31.0	41.0
16	36.638334248791296	37.0	35.0	40.0	32.0	41.0
17	36.616560936302236	37.0	35.0	40.0	32.0	41.0
18	36.594058778437045	37.0	35.0	40.0	32.0	41.0
19	36.50422971757619	37.0	35.0	40.0	32.0	41.0
20	36.31395127751655	36.0	35.0	40.0	32.0	41.0
21	36.10074514253982	36.0	35.0	40.0	31.0	41.0
22	35.99644933091089	36.0	35.0	40.0	31.0	41.0
23	36.0404803438098	35.0	35.0	40.0	31.0	41.0
24	36.05268812661085	35.0	35.0	40.0	31.0	41.0
25	35.99519837269803	35.0	35.0	40.0	31.0	41.0
26	35.85984741025997	35.0	35.0	40.0	31.0	41.0
27	35.77692804500432	35.0	34.0	40.0	31.0	41.0
28	35.811965437941005	36.0	35.0	40.0	31.0	41.0
29	35.83878483518739	36.0	35.0	40.0	31.0	41.0
30	35.80397530074303	36.0	35.0	40.0	31.0	41.0
31	35.59995442830154	36.0	35.0	40.0	30.0	41.0
32	35.388373482703884	35.0	34.0	40.0	30.0	41.0
33	35.25633778587941	35.0	34.0	40.0	30.0	41.0
34	35.17514622177422	35.0	34.0	40.0	29.0	41.0
35	35.07764693100264	35.0	34.0	40.0	29.0	41.0
36	34.85858136208073	35.0	34.0	40.0	27.0	41.0
37	34.756268372034064	35.0	34.0	40.0	27.0	41.0
38	34.711279146270066	35.0	34.0	40.0	27.0	41.0
39	34.737421004026004	35.0	34.0	40.0	27.0	41.0
40	34.631748987463254	35.0	34.0	40.0	27.0	41.0
41	34.59916069606997	35.0	34.0	40.0	26.0	41.0
42	34.50749065931081	35.0	34.0	40.0	26.0	41.0
43	34.33932807397705	35.0	33.0	40.0	25.0	41.0
44	34.175250342542235	35.0	34.0	40.0	24.0	41.0
45	34.14355537715862	35.0	34.0	40.0	24.0	41.0
46	34.05010774235995	35.0	34.0	40.0	24.0	41.0
47	33.962732310032415	35.0	33.0	39.0	24.0	41.0
48	33.893258104820944	35.0	33.0	39.0	23.0	41.0
49	33.843091511592114	35.0	33.0	39.0	24.0	41.0
50	33.653940292021026	35.0	33.0	39.0	24.0	41.0
51	33.20955587210952	35.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	3.0
14	5.0
15	13.0
16	36.0
17	98.0
18	223.0
19	475.0
20	876.0
21	1456.0
22	2358.0
23	3553.0
24	5188.0
25	7782.0
26	10060.0
27	11833.0
28	12968.0
29	14946.0
30	17491.0
31	21722.0
32	27365.0
33	36529.0
34	71177.0
35	135096.0
36	38922.0
37	45474.0
38	68703.0
39	128318.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.40719972475901	26.209611704985118	29.754546606870157	12.628641963385705
2	29.672306290101584	26.580070379603193	30.95268993740682	12.7949333928884
3	27.736414503268485	26.79117900925317	31.157158981849786	14.315247505628555
4	25.059907166526834	28.011504590367775	31.95134391240576	14.977244330699632
5	22.718246183747503	31.705075661091424	31.08865053448661	14.488027620674462
6	22.139847772419163	41.21990306205598	26.391898498850146	10.248350666674714
7	87.18680774779234	4.273176679362358	6.552817900321719	1.9871976725235858
8	88.01237980841779	3.094801204782916	6.36117532730137	2.5316436594979264
9	84.70269748239001	5.165295491721644	7.557206062544893	2.5748009633434537
10	55.26685096545605	23.776807325273282	12.339065508562047	8.617276200708625
11	51.510052935602054	18.31363589721922	18.858534583184948	11.317776583993771
12	45.85780422881218	20.595087914144127	20.36587132483869	13.181236532205007
13	21.3316291731302	45.651524388403665	21.147531583299635	11.869314855166504
14	13.37438810186331	48.367416537395954	25.268450501892282	12.989744858848454
15	10.245332673398803	25.10608246364827	52.46705860339343	12.181526259559494
16	12.591520646092	19.760009174699558	51.14351765224268	16.50495252696577
17	14.01918236526169	20.080821859928896	27.92881157460781	37.971184200201606
18	20.36813481979562	23.404839654017252	36.15148515449108	20.07554037169605
19	28.71741321760335	26.734138936338447	26.05388325194811	18.494564594110084
20	33.98728217633531	24.99185141815504	23.996668135423395	17.024198270086256
21	22.060927248254092	29.88039692647565	28.240570280009415	19.818105545260845
22	20.84165796478605	24.86237950661846	26.486965287041343	27.808997241554145
23	15.84114490592915	31.415801005595362	25.94040670477386	26.80264738370163
24	18.398592407936114	24.58170613195874	40.9479818678964	16.07171959220875
25	13.520308076753604	25.821648669366766	39.38315235433655	21.274890899543074
26	13.699275078015127	39.746367845092436	27.182763636802616	19.371593440089814
27	15.768411267979696	39.092821401193916	30.520664199960162	14.618103130866226
28	11.982187803685573	29.790913425844888	43.13678149125084	15.0901172792187
29	12.511996523271746	25.419199266024034	42.116699763992926	19.952104446711296
30	16.540715747285315	33.748558908210754	33.41597604920536	16.29474929529857
31	31.115963373633605	26.772165651614927	24.92651186373157	17.185359111019903
32	31.479329764053286	28.03866652985097	24.889239646774065	15.592764059321675
33	28.711226331387735	28.797390039414992	23.76654614813518	18.724837481062092
34	17.06735557393178	29.020419742504817	27.799037863743642	26.113186819819767
35	16.872242308644136	27.00470203352387	31.626155136926354	24.49690052090564
36	30.428917204372468	22.279429961430047	30.942881459260107	16.348771374937378
37	17.781261883348524	32.316068399799605	32.86790847029993	17.034761246551945
38	15.857894768610455	34.95092742933369	25.102008172725792	24.089169629330065
39	16.659473782692412	33.2145249980383	31.06556288592589	19.060438333343395
40	24.416471000102614	27.34226458143451	24.709065448202182	23.532198970260694
41	15.730837251694604	22.516040634261465	29.850971492035516	31.902150622008413
42	21.78055567292196	22.04598818153833	26.269820670839543	29.903635474700163
43	21.328912979181883	23.578977866037317	26.926234208350188	28.16587494643062
44	17.53831342463769	30.788360203533465	29.733420653938786	21.93990571789006
45	15.65870721240033	41.04697204734628	22.99937225739861	20.294948482854778
46	23.170341576478968	34.14783941861377	24.29288417545406	18.3889348294532
47	17.582979725121174	29.887338311010243	26.81849184840016	25.711190115468423
48	21.970538349640556	23.162343894297805	33.814200261961815	21.052917494099823
49	19.709910486319437	21.66768272440289	35.53430552956728	23.088101259710395
50	17.992974111653677	33.09682326027777	28.865445787786786	20.04475684028176
51	15.166019810107864	36.998334067711696	24.470945778732805	23.364700343447634
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	636.0
1	747.5
2	859.0
3	2924.0
4	4989.0
5	3622.0
6	2255.0
7	2497.5
8	2740.0
9	3068.0
10	3396.0
11	3628.5
12	3861.0
13	3839.5
14	3818.0
15	3535.5
16	3253.0
17	2914.5
18	2576.0
19	2606.5
20	2637.0
21	2378.5
22	2120.0
23	2077.5
24	2035.0
25	2595.0
26	3698.0
27	4241.0
28	4937.0
29	5633.0
30	7869.0
31	10105.0
32	10953.5
33	11802.0
34	13244.5
35	14687.0
36	16670.0
37	18653.0
38	22235.0
39	25817.0
40	41301.5
41	56786.0
42	70438.5
43	84091.0
44	87827.5
45	91564.0
46	84038.0
47	76512.0
48	72177.5
49	67843.0
50	59484.5
51	51126.0
52	44022.0
53	36918.0
54	30570.0
55	24222.0
56	19965.5
57	15709.0
58	13433.5
59	11158.0
60	9082.0
61	7006.0
62	5550.0
63	4094.0
64	3447.0
65	2800.0
66	2159.5
67	1519.0
68	1282.0
69	1045.0
70	727.5
71	410.0
72	349.0
73	288.0
74	222.0
75	128.0
76	100.0
77	67.5
78	35.0
79	25.5
80	16.0
81	13.0
82	10.0
83	9.5
84	9.0
85	5.0
86	1.0
87	2.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	662692.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.608129143753757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.28136886094228	18.716847947627034
2	9.268979803924445	4.376465444837875
3	3.403610201235896	2.4105860755732427
4	1.5846880138732853	1.4964607713631668
5	0.9187432912469201	1.0844905134857332
6	0.5404016996841575	0.7654723869388577
7	0.4022782412651992	0.6647925670057669
8	0.30157962495459256	0.5695784586442276
9	0.24046882724735466	0.5109317215832305
>10	2.03377486688744	11.126348530697266
>50	0.8870688698967533	15.511481918304046
>100	1.1312691874044578	41.277752504023134
>500	0.004486620006703232	0.6122216722250117
>1k	0.0012818914304866375	0.8765694876914217
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3662	0.5525945688193007	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2131	0.32156718354831504	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5089966379554907E-4	0.0	0.0	0.15044696480416242	0.0
2	1.5089966379554907E-4	0.0	0.0	0.5461058832760921	0.0
3	1.5089966379554907E-4	0.0	0.0	0.7901106396334949	0.0
4	1.5089966379554907E-4	0.0	0.0	1.2222872767439474	0.0
5	1.5089966379554907E-4	0.0	0.0	2.025224387800064	0.0
6	1.5089966379554907E-4	0.0	0.0	2.6703204505260363	0.0
7	1.5089966379554907E-4	0.0	0.0	3.04922950631666	0.0
8	1.5089966379554907E-4	0.0	0.0	3.940442920693173	0.0
9	1.5089966379554907E-4	0.0	0.0	4.183089580076415	0.0
10	1.5089966379554907E-4	0.0	0.0	4.703089821515878	0.0
11	1.5089966379554907E-4	0.0	0.0	5.611807596892674	0.0
12	1.5089966379554907E-4	0.0	0.0	6.230798017782017	0.0
13	1.5089966379554907E-4	0.0	0.0	6.4874783458982455	0.0
14	1.5089966379554907E-4	0.0	0.0	6.576660047201415	0.0
15	1.5089966379554907E-4	0.0	0.0	6.729068707634919	0.0
16	1.5089966379554907E-4	0.0	0.0	7.079005027976797	0.0
17	1.5089966379554907E-4	0.0	0.0	7.50137318694054	0.0
18	1.5089966379554907E-4	0.0	0.0	7.9770089272241105	0.0
19	1.5089966379554907E-4	0.0	0.0	8.241536037857708	0.0
20	1.5089966379554907E-4	0.0	0.0	8.514815328991448	0.0
21	1.5089966379554907E-4	0.0	0.0	8.889951893187183	0.0
22	1.5089966379554907E-4	0.0	0.0	9.28123472140904	0.0
23	1.5089966379554907E-4	0.0	0.0	9.696963295165778	0.0
24	1.5089966379554907E-4	0.0	0.0	9.97899476679966	0.0
25	1.5089966379554907E-4	0.0	0.0	10.205193362829188	0.0
26	1.5089966379554907E-4	0.0	0.0	10.431391958858717	0.0
27	1.5089966379554907E-4	0.0	0.0	10.649140173715693	0.0
28	1.5089966379554907E-4	0.0	0.0	10.873829773107266	0.0
29	1.5089966379554907E-4	0.0	0.0	11.135640689792544	0.0
30	1.5089966379554907E-4	0.0	0.0	11.462489361573702	0.0
31	1.5089966379554907E-4	0.0	0.0	11.780133153863334	0.0
32	1.5089966379554907E-4	0.0	0.0	12.04269856886759	0.0
33	1.5089966379554907E-4	0.0	0.0	12.305263983871845	0.0
34	1.5089966379554907E-4	0.0	0.0	12.586239157859156	0.0
35	1.5089966379554907E-4	0.0	0.0	12.952170842563362	0.0
36	1.5089966379554907E-4	0.0	0.0	13.239182003102497	0.0
37	1.5089966379554907E-4	0.0	0.0	13.53268184918484	0.0
38	1.5089966379554907E-4	0.0	0.0	13.790267575283842	0.0
39	1.5089966379554907E-4	0.0	0.0	14.080447628762682	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAACC	30	2.1641044E-6	45.000004	6
TACCCTA	30	2.1641044E-6	45.000004	11
GATACCA	125	0.0	45.000004	9
GGTACCG	30	2.1641044E-6	45.000004	1
CACGGGT	30	2.1641044E-6	45.000004	4
GCGCGAG	85	0.0	45.000004	1
GCGCGAC	30	2.1641044E-6	45.000004	9
TTTCGCG	30	2.1641044E-6	45.000004	2
TTCACGC	30	2.1641044E-6	45.000004	40
TAACGTG	30	2.1641044E-6	45.000004	15
CGCGACC	30	2.1641044E-6	45.000004	10
ATGTCGC	30	2.1641044E-6	45.000004	33
TCTCGCG	215	0.0	45.000004	32
AACGACG	30	2.1641044E-6	45.000004	1
CTCGCGC	215	0.0	45.000004	33
ACGCTCG	30	2.1641044E-6	45.000004	1
ACTCGCG	30	2.1641044E-6	45.000004	1
GTTCACG	30	2.1641044E-6	45.000004	39
CATCGGT	30	2.1641044E-6	45.000004	37
GGCGCGA	30	2.1641044E-6	45.000004	8
>>END_MODULE