FastQCFastQC Report
Thu 26 May 2016
SRR1548119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548119_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1362952
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44220.3244428270401305No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG30620.2246594157387788No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG18270.13404727385850712No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT18170.13331357230482072No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC16580.12164771760120678No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC207.0337474E-445.00000417
TAGACCC207.0337474E-445.00000422
CGGCACA253.891188E-545.00000419
CCTAACG253.891188E-545.00000433
TTATCCG302.1657997E-645.0000041
CCGTCAG253.891188E-545.00000425
ATATACG406.8175723E-945.0000041
TCCGTCG302.1657997E-645.0000044
TATAGCG900.045.0000041
CCGCGAC207.0337474E-445.00000424
ATCGGGT302.1657997E-645.00000422
CGCAATA253.891188E-545.00000428
TGAATCG207.0337474E-445.00000441
GCGCTAA207.0337474E-445.00000443
TATCGTT207.0337474E-445.00000415
CAATACG207.0337474E-445.0000041
AGTACGG1250.045.0000042
GCGTAAT253.891188E-545.00000426
GATTACG1050.045.01
CGGTCTA2150.043.9534931