##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548119_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1362952
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.539319799963607	31.0	31.0	33.0	30.0	34.0
2	31.944564445409668	31.0	31.0	34.0	30.0	34.0
3	32.04922623834148	33.0	31.0	34.0	30.0	34.0
4	35.75064492366569	37.0	35.0	37.0	35.0	37.0
5	35.64720401011921	37.0	35.0	37.0	33.0	37.0
6	35.713875470302696	37.0	35.0	37.0	33.0	37.0
7	36.05780174210097	37.0	35.0	37.0	35.0	37.0
8	36.07999254559221	37.0	35.0	37.0	35.0	37.0
9	37.89870296239339	39.0	38.0	39.0	35.0	39.0
10	37.37099692432309	39.0	37.0	39.0	35.0	39.0
11	37.01641363745752	39.0	37.0	39.0	33.0	39.0
12	36.27348945524127	38.0	35.0	39.0	32.0	39.0
13	35.975138522853335	38.0	35.0	39.0	32.0	39.0
14	37.033657091372255	39.0	35.0	41.0	32.0	41.0
15	37.26052568248919	39.0	35.0	41.0	32.0	41.0
16	37.400399280385514	39.0	35.0	41.0	33.0	41.0
17	37.35819309850971	39.0	35.0	41.0	33.0	41.0
18	37.30859927568983	39.0	35.0	41.0	32.0	41.0
19	37.25185479752772	39.0	35.0	41.0	32.0	41.0
20	37.12887981381589	39.0	35.0	41.0	32.0	41.0
21	36.97960823271839	39.0	35.0	41.0	32.0	41.0
22	36.87024634763367	38.0	35.0	41.0	32.0	41.0
23	36.81020314728619	38.0	35.0	41.0	32.0	41.0
24	36.834217199138344	38.0	35.0	41.0	32.0	41.0
25	36.815260552095744	38.0	35.0	41.0	32.0	41.0
26	36.67398118202255	38.0	35.0	41.0	31.0	41.0
27	36.61654335589221	38.0	35.0	41.0	31.0	41.0
28	36.59102154734723	38.0	35.0	41.0	31.0	41.0
29	36.57320067030974	38.0	35.0	40.0	31.0	41.0
30	36.51659339433817	38.0	35.0	40.0	31.0	41.0
31	36.433391638150134	38.0	35.0	41.0	31.0	41.0
32	36.29117092898356	38.0	35.0	41.0	30.0	41.0
33	36.22723911040154	38.0	35.0	41.0	30.0	41.0
34	36.149418321408234	38.0	35.0	41.0	30.0	41.0
35	36.05231071967318	38.0	35.0	41.0	30.0	41.0
36	35.90424167542217	38.0	35.0	40.0	30.0	41.0
37	35.803503718399476	38.0	35.0	40.0	29.0	41.0
38	35.734315661886846	38.0	35.0	40.0	29.0	41.0
39	35.71786974156097	38.0	35.0	40.0	29.0	41.0
40	35.57183451801678	38.0	35.0	40.0	29.0	41.0
41	35.556395969924104	38.0	34.0	40.0	29.0	41.0
42	35.512916082151094	38.0	34.0	40.0	28.0	41.0
43	35.433730608267936	38.0	34.0	40.0	28.0	41.0
44	35.25509115508103	37.0	34.0	40.0	27.0	41.0
45	35.21685503231222	37.0	34.0	40.0	27.0	41.0
46	35.142649190873925	37.0	34.0	40.0	27.0	41.0
47	35.07783106081506	37.0	34.0	40.0	27.0	41.0
48	34.99160498682272	37.0	34.0	40.0	27.0	41.0
49	34.89765523657473	36.0	34.0	40.0	27.0	41.0
50	34.72163363053138	36.0	34.0	40.0	26.0	41.0
51	34.35942498341834	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	7.0
13	6.0
14	19.0
15	38.0
16	87.0
17	197.0
18	450.0
19	913.0
20	1635.0
21	2656.0
22	4029.0
23	6020.0
24	8588.0
25	12510.0
26	16215.0
27	19454.0
28	22147.0
29	25826.0
30	30975.0
31	38481.0
32	48088.0
33	63715.0
34	114169.0
35	183563.0
36	88677.0
37	117039.0
38	188856.0
39	368503.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.23726734323733	24.33064407257189	29.567585652319377	12.864502931871408
2	29.228175313584043	26.732269368253615	30.654564504105792	13.384990814056547
3	30.00362448567521	25.76018817977449	30.162837722825163	14.073349611725138
4	27.502509259313605	27.271173159436284	30.142220709166573	15.084096872083535
5	23.836862926940935	33.51673426503648	28.26042296427167	14.385979843750917
6	22.660739336381617	41.82480380820455	25.386220497860528	10.12823635755331
7	89.7727873028544	4.0885519079175205	4.642716691416866	1.4959440978112215
8	90.53862498459226	3.4631447035552245	4.363983471171399	1.6342468406811097
9	85.30667257540985	6.764581584677963	5.550012032705481	2.37873380720671
10	46.093039226619865	35.39713797697938	10.24328076117134	8.266542035229413
11	40.392545005253304	21.40332161367385	23.40361215948911	14.80052122158374
12	39.10335800527091	21.082033703314572	24.052130962792525	15.76247732862199
13	24.04266621274997	35.362947484577596	23.572290146681617	17.02209615599082
14	17.33876174656187	37.582247944168245	26.000255328140682	19.078734981129198
15	15.979652988513168	28.871302877870974	40.039781298240875	15.109262835374981
16	19.64104385187446	24.457574441359636	38.91010101602991	16.99128069073599
17	18.995166374164313	25.745294038234658	26.433285985126403	28.826253602474626
18	22.290586902546824	28.429761282862493	30.16349805422348	19.1161537603672
19	27.868332853981652	28.476498071832317	24.344877882713405	19.31029119147263
20	30.79778304738538	26.579512704776104	25.440734523299426	17.18196972453909
21	24.53835498242051	28.812386643109956	27.8230634681192	18.82619490635033
22	24.502917197377457	24.928464098515573	25.749696247556773	24.818922456550194
23	21.707147427055393	29.247178183824524	26.59403999553909	22.451634393580992
24	20.73051728894341	25.770019780593888	35.720553621844346	17.778909308618353
25	19.36194378085215	29.12420980342668	31.070573285045988	20.443273130675184
26	18.895089482241488	36.27794669217991	24.815033838315657	20.011929987262942
27	19.127892985226186	35.73625483509324	27.516302848522912	17.619549331157664
28	16.20937494497238	31.03917085854821	35.144304421579044	17.607149774900364
29	17.564008123543605	26.701674013464892	34.69454536916927	21.039772493822234
30	19.708984615745823	30.85611232090345	30.02989100129718	19.405012062053544
31	28.065258350991083	28.35910582324249	24.33269843692221	19.242937388844215
32	27.827172196819845	29.219004044162965	25.64103504745582	17.312788711561375
33	25.543452740815525	30.119402590846928	24.57555365119241	19.76159101714514
34	20.111346547787452	29.22083829804718	27.928936602316153	22.738878551849222
35	21.15063479858425	26.872479735163086	29.7678128063204	22.209072659932264
36	27.56443367044474	27.946912290381466	25.935249370484065	18.553404668689726
37	20.989954158326928	33.185468013547066	28.178468500724897	17.64610932740111
38	20.770797504240797	32.02145049862358	25.399500495982252	21.80825150115338
39	20.524714003134374	31.31783070863831	27.958578145085077	20.198877143142237
40	23.884920378707395	27.848963132964332	26.42800333393986	21.838113154388417
41	18.086403629768327	26.0133152157963	29.341091982696383	26.55918917173899
42	20.9221601347663	27.441098439270057	25.42224524414653	26.214496181817115
43	22.30386690066855	27.284820008334847	26.20503143177456	24.206281659222043
44	20.226611061871587	31.084220133944555	27.767742370971245	20.921426433212613
45	19.499659562479092	36.025773468177896	23.69841344376031	20.776153525582703
46	21.287029917414554	33.15663354248719	25.29648879784468	20.259847742253577
47	21.1809366727515	28.79008211587789	27.467511695202766	22.561469516167847
48	22.215089012672493	25.184085719819922	31.54601189183478	21.054813375672804
49	20.680405472826628	26.712826277080925	30.587577552254224	22.019190697838223
50	19.304494949198507	31.81278577675516	28.32080660214006	20.561912671906274
51	18.699117797251848	31.569490341552747	26.531822103786485	23.19956975740892
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	483.0
1	1338.0
2	2193.0
3	5040.5
4	7888.0
5	5988.5
6	4089.0
7	3936.5
8	3784.0
9	3872.0
10	3960.0
11	3978.5
12	3997.0
13	4024.5
14	4052.0
15	4000.5
16	3949.0
17	3759.5
18	3570.0
19	3325.5
20	3081.0
21	4081.0
22	5081.0
23	5056.0
24	5031.0
25	7246.0
26	12122.5
27	14784.0
28	20277.0
29	25770.0
30	28885.5
31	32001.0
32	37133.5
33	42266.0
34	44804.0
35	47342.0
36	48466.0
37	49590.0
38	56276.5
39	62963.0
40	80438.0
41	97913.0
42	112180.0
43	126447.0
44	131044.0
45	135641.0
46	125652.5
47	115664.0
48	109679.5
49	103695.0
50	96801.0
51	89907.0
52	81759.0
53	73611.0
54	66919.5
55	60228.0
56	56512.0
57	52796.0
58	50643.0
59	48490.0
60	45926.0
61	43362.0
62	36595.5
63	29829.0
64	24390.5
65	18952.0
66	15210.0
67	11468.0
68	9485.5
69	7503.0
70	6175.0
71	4847.0
72	4030.0
73	3213.0
74	2643.0
75	1602.0
76	1131.0
77	791.5
78	452.0
79	333.0
80	214.0
81	159.0
82	104.0
83	67.5
84	31.0
85	27.5
86	24.0
87	15.0
88	6.0
89	7.0
90	8.0
91	6.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1362952.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.21402863251074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.53248116668671	15.665674276163614
2	7.546500435518334	2.899973508866081
3	2.192564018207303	1.2638396347334375
4	0.9869527571482533	0.7585335413912783
5	0.5229325637097918	0.5023820625996094
6	0.36659223579316397	0.4226228228991589
7	0.25359436583799316	0.3410798584378223
8	0.2210992930641626	0.3398566518050163
9	0.18013417982758145	0.311499296001089
>10	2.983154805618176	15.212400620283997
>50	1.958725155412607	27.523657307109772
>100	1.2472273363282809	32.95170605198148
>500	0.005361124565120029	0.679710930488189
>1k	0.0026805622825600145	1.1270634372394541
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4422	0.3244428270401305	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3062	0.2246594157387788	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1827	0.13404727385850712	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1817	0.13331357230482072	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1658	0.12164771760120678	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03154916680851563	0.0
2	0.0	0.0	0.0	0.10968838227611831	0.0
3	0.0	0.0	0.0	0.1628083747630144	0.0
4	0.0	0.0	0.0	0.24571665032957873	0.0
5	0.0	0.0	0.0	0.4220251336804231	0.0
6	0.0	0.0	0.0	0.5789638960139462	0.0
7	0.0	0.0	0.0	0.6800679701119335	0.0
8	0.0	0.0	0.0	0.901939319946704	0.0
9	0.0	0.0	0.0	0.9651110237191038	0.0
10	0.0	0.0	0.0	1.110383931349013	0.0
11	0.0	0.0	0.0	1.3381248936132748	0.0
12	0.0	0.0	0.0	1.5082702839131532	0.0
13	0.0	0.0	0.0	1.5754039760754597	0.0
14	0.0	0.0	0.0	1.6048987785336535	0.0
15	0.0	0.0	0.0	1.6609535772352952	0.0
16	0.0	0.0	0.0	1.7749707986781633	0.0
17	0.0	0.0	0.0	1.9268470202912502	0.0
18	0.0	0.0	0.0	2.1056500889246283	0.0
19	0.0	0.0	0.0	2.2059470913135604	0.0
20	0.0	0.0	0.0	2.3159289542111536	0.0
21	0.0	0.0	0.0	2.45701976298505	0.0
22	0.0	0.0	0.0	2.621735761787649	0.0
23	0.0	0.0	0.0	2.785204467948981	0.0
24	0.0	0.0	0.0	2.9049445615106033	0.0
25	0.0	0.0	0.0	3.0048747131226925	0.0
26	0.0	0.0	0.0	3.1064190081528915	0.0
27	0.0	0.0	0.0	3.2037078341717096	0.0
28	0.0	0.0	0.0	3.31545058079815	0.0
29	0.0	0.0	0.0	3.4393727732157844	0.0
30	0.0	0.0	0.0	3.597045237102994	0.0
31	0.0	0.0	0.0	3.7510491932217715	0.0
32	0.0	0.0	0.0	3.888031273295024	0.0
33	0.0	0.0	0.0	4.015181752548879	0.0
34	0.0	0.0	0.0	4.156125821012038	0.0
35	0.0	0.0	0.0	4.32773861441929	0.0
36	0.0	0.0	0.0	4.483723564733021	0.0
37	0.0	0.0	0.0	4.641689509241705	0.0
38	0.0	0.0	0.0	4.803470701829558	0.0
39	0.0	0.0	0.0	4.967966590166052	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	20	7.0337474E-4	45.000004	17
TAGACCC	20	7.0337474E-4	45.000004	22
CGGCACA	25	3.891188E-5	45.000004	19
CCTAACG	25	3.891188E-5	45.000004	33
TTATCCG	30	2.1657997E-6	45.000004	1
CCGTCAG	25	3.891188E-5	45.000004	25
ATATACG	40	6.8175723E-9	45.000004	1
TCCGTCG	30	2.1657997E-6	45.000004	4
TATAGCG	90	0.0	45.000004	1
CCGCGAC	20	7.0337474E-4	45.000004	24
ATCGGGT	30	2.1657997E-6	45.000004	22
CGCAATA	25	3.891188E-5	45.000004	28
TGAATCG	20	7.0337474E-4	45.000004	41
GCGCTAA	20	7.0337474E-4	45.000004	43
TATCGTT	20	7.0337474E-4	45.000004	15
CAATACG	20	7.0337474E-4	45.000004	1
AGTACGG	125	0.0	45.000004	2
GCGTAAT	25	3.891188E-5	45.000004	26
GATTACG	105	0.0	45.0	1
CGGTCTA	215	0.0	43.95349	31
>>END_MODULE