FastQCFastQC Report
Thu 26 May 2016
SRR1548118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548118_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1520465
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313682.063053079156705No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTT27770.18264149454278789No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26770.1760645591973508No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21930.1442321921254353No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTC20030.13173601496910484No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCT20020.1316702456156505No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18060.1187794523385938No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG16930.11134751539824987No Hit
CGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTG15830.10411288651826908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG302.1659653E-645.0000042
AGCGCGT207.034006E-445.037
ATCGCCG207.034006E-445.026
TAGTACG351.2122837E-745.01
CGTTTTT108750.044.0689661
TACGGGA6650.042.2932324
CGACGGT6350.041.81102428
AACGCCG2050.041.70731713
TCACGAC6450.041.5116325
GACGGTC6450.041.5116329
CGCATCG1900.041.4473721
ACGGTCT6450.041.16279230
CACGACG6400.041.13281226
GCGCGAC2850.041.052639
GGCCGAT2200.040.909098
CGGTCTA6550.040.8778631
CCAATCG1050.040.71428724
CCGCTCG1000.040.519
CGCCGAC1000.040.513
TAACGCC2300.040.10869212