##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548118_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1520465
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4140568839138	31.0	31.0	33.0	30.0	34.0
2	31.77569559312447	31.0	31.0	34.0	30.0	34.0
3	31.614712604367742	31.0	31.0	34.0	30.0	34.0
4	35.47299082846366	37.0	35.0	37.0	33.0	37.0
5	35.43777660123712	37.0	35.0	37.0	33.0	37.0
6	35.53382287655421	37.0	35.0	37.0	33.0	37.0
7	36.00889201658703	37.0	35.0	37.0	35.0	37.0
8	36.06205798883894	37.0	35.0	37.0	35.0	37.0
9	37.87386753394521	39.0	38.0	39.0	35.0	39.0
10	37.26077285567244	39.0	37.0	39.0	34.0	39.0
11	36.91248269443887	39.0	37.0	39.0	33.0	39.0
12	36.50973024699681	39.0	35.0	39.0	32.0	39.0
13	36.34854929248618	39.0	35.0	39.0	32.0	39.0
14	37.32623638163325	40.0	36.0	41.0	32.0	41.0
15	37.52875797864469	40.0	36.0	41.0	32.0	41.0
16	37.628213737244856	40.0	36.0	41.0	32.0	41.0
17	37.56473841883897	40.0	35.0	41.0	32.0	41.0
18	37.422113629711966	39.0	36.0	41.0	32.0	41.0
19	37.29236121844304	39.0	36.0	41.0	32.0	41.0
20	37.06727349856787	39.0	35.0	41.0	32.0	41.0
21	36.95327350514481	39.0	35.0	41.0	32.0	41.0
22	36.905822889708084	38.0	35.0	41.0	32.0	41.0
23	36.81566889076697	38.0	35.0	41.0	31.0	41.0
24	36.80181523415534	38.0	35.0	41.0	31.0	41.0
25	36.76340987789919	38.0	35.0	41.0	31.0	41.0
26	36.650047189511106	38.0	35.0	41.0	31.0	41.0
27	36.60420792323401	38.0	35.0	40.0	31.0	41.0
28	36.52247569000273	38.0	35.0	40.0	31.0	41.0
29	36.409111028534035	38.0	35.0	40.0	31.0	41.0
30	36.25496344868182	38.0	35.0	40.0	30.0	41.0
31	36.071378821610494	38.0	35.0	40.0	30.0	41.0
32	35.85500948722924	38.0	35.0	40.0	29.0	41.0
33	35.634813691864004	38.0	35.0	40.0	28.0	41.0
34	35.37860654470836	38.0	34.0	40.0	26.0	41.0
35	35.15779449050126	38.0	34.0	40.0	25.0	41.0
36	34.95687240416583	38.0	34.0	40.0	24.0	41.0
37	34.84684290661081	38.0	34.0	40.0	23.0	41.0
38	34.634521675934664	38.0	34.0	40.0	23.0	41.0
39	34.56873982630314	38.0	34.0	40.0	23.0	41.0
40	34.422630576830116	38.0	33.0	40.0	22.0	41.0
41	34.33606889997468	37.0	33.0	40.0	22.0	41.0
42	34.31049514457748	37.0	33.0	40.0	22.0	41.0
43	34.205791649265194	37.0	33.0	40.0	22.0	41.0
44	34.01232320375674	37.0	33.0	40.0	21.0	41.0
45	33.9989661057637	37.0	33.0	40.0	21.0	41.0
46	33.93889961294735	37.0	33.0	40.0	21.0	41.0
47	33.83366864742036	37.0	33.0	40.0	20.0	41.0
48	33.64417135547349	36.0	33.0	40.0	20.0	41.0
49	33.519195114652426	36.0	33.0	40.0	20.0	41.0
50	33.337855853308035	36.0	32.0	40.0	19.0	41.0
51	33.00542794474059	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	6.0
14	17.0
15	48.0
16	115.0
17	342.0
18	710.0
19	1399.0
20	2496.0
21	4193.0
22	6522.0
23	9870.0
24	15231.0
25	22639.0
26	30231.0
27	33818.0
28	34036.0
29	35770.0
30	40222.0
31	46779.0
32	56549.0
33	70330.0
34	107800.0
35	156155.0
36	114328.0
37	141457.0
38	212053.0
39	377290.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.82030826095964	23.798245931343374	27.8110314936549	17.570414314042086
2	34.89425932198373	24.464884097956872	28.404994524701326	12.235862055358066
3	27.771635650935732	24.412071307133015	35.23007764072175	12.5862154012095
4	25.106003755430084	25.966135359906346	35.274932339777635	13.65292854488594
5	22.239972639948963	29.95570434044848	33.90094477676237	13.903378242840184
6	20.222168875968865	39.248913983551084	30.571502796841756	9.957414343638295
7	84.83963787393988	2.6472822458918817	10.841880608892675	1.6711992712755637
8	85.57217693271465	1.9691344424238637	10.52342539946661	1.9352632253948627
9	80.84270272581085	4.624966704264813	11.92839032795888	2.6039402419654514
10	38.24448441759593	33.87358472572535	17.787979335269142	10.093951521409569
11	27.98847720927479	23.379623996606302	31.641044022716734	16.99085477140217
12	28.320349366805548	21.309467827276524	32.49946562400318	17.870717181914745
13	23.190339797364622	25.644720529574833	33.075539390910016	18.089400282150525
14	16.953695086700453	30.258506443752403	33.830242721798925	18.957555747748223
15	15.754982850641087	28.30923434607176	39.303831393685485	16.631951409601665
16	21.151818687046397	25.973369988786327	37.52687500205529	15.347936322111986
17	20.77430259821831	26.1769261377276	30.668578362540405	22.38019290151368
18	21.099005896222536	28.50220162910688	33.17051033729813	17.22828213737245
19	23.079452667440552	28.96508633871875	29.964846280578637	17.99061471326206
20	23.647305265165592	29.72472237111673	29.034275698552747	17.59369666516493
21	22.69332079330994	27.58623184354786	33.18267766768719	16.537769695455008
22	21.3632671584022	23.6135655868435	33.66253087049028	21.360636384264026
23	18.671524829575162	28.420318784056192	33.163867632599235	19.744288753769407
24	19.523961419697265	28.189928738905536	34.955687898110114	17.330421943287085
25	20.75963603239798	27.97229794832502	32.95202454512271	18.31604147415429
26	17.91353303101354	31.90221412528404	29.42994412893424	20.75430871476818
27	18.74472611996988	30.889760698207457	32.43665589145426	17.92885729036841
28	16.69502421956441	29.331618945519956	35.241850354990085	18.73150647992555
29	18.23817055966431	28.746732085250237	33.914361724867064	19.100735630218388
30	21.21265533899169	28.386118720259923	32.87744209830545	17.523783842442935
31	24.112820748915627	27.867856215039478	29.866981482638533	18.15234155340636
32	24.53854577382577	29.43145682406369	28.65241883239667	17.37757856971387
33	23.402445962254966	29.21329987865554	29.000272942816835	18.383981216272655
34	18.84042052924599	29.78628248595002	31.818818585103898	19.55447839970009
35	19.773753424116965	29.514260440062746	30.6910714814218	20.02091465439849
36	24.097167642793487	29.76760398956898	28.85327843784632	17.281949929791214
37	20.423094250771967	31.76482194591786	29.832452572074992	17.97963123123518
38	20.562854126862508	32.26078864031727	28.38881526375155	18.787541969068673
39	19.860108585202553	30.122429651455313	29.007376032989907	21.010085730352227
40	22.330273962241815	27.76177024791758	30.495473424248505	19.412482365592105
41	19.33296721726577	27.37747991568369	30.451605265494436	22.837947601556102
42	20.85269966753592	28.8492665072856	28.82999608672347	21.468037738455013
43	20.710703633427933	28.36277059978362	29.178179043910905	21.74834672287754
44	21.0209376736722	30.04909682235369	29.278740385342644	19.651225118631473
45	19.85136126119312	31.271683333716986	27.380307997882227	21.496647407207664
46	21.741375171411377	30.447001410752634	27.84983541219298	19.96178800564301
47	20.710900941488294	29.44165107384912	29.093665424722044	20.753782559940547
48	19.932520643355815	30.220952142929956	29.87776765660505	19.968759557109173
49	21.008244188455507	28.520880125487924	29.734522004781432	20.736353681275137
50	19.171240377121475	29.3427997356072	30.77354625065358	20.712413636617747
51	17.841515588981004	31.08937068594147	29.519061602864916	21.550052122212612
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	940.0
1	1775.0
2	2610.0
3	21981.0
4	41352.0
5	27540.5
6	13729.0
7	12481.5
8	11234.0
9	11977.0
10	12720.0
11	13431.5
12	14143.0
13	14547.0
14	14951.0
15	14546.5
16	14142.0
17	13468.5
18	12795.0
19	12004.5
20	11214.0
21	11242.5
22	11271.0
23	11876.5
24	12482.0
25	13395.0
26	15903.0
27	17498.0
28	20603.0
29	23708.0
30	27378.5
31	31049.0
32	34535.5
33	38022.0
34	42125.0
35	46228.0
36	48489.0
37	50750.0
38	57307.5
39	63865.0
40	71996.5
41	80128.0
42	91427.5
43	102727.0
44	109635.5
45	116544.0
46	123761.0
47	130978.0
48	135238.5
49	139499.0
50	130379.0
51	121259.0
52	106940.0
53	92621.0
54	84229.5
55	75838.0
56	68286.5
57	60735.0
58	55217.5
59	49700.0
60	42386.0
61	35072.0
62	29337.0
63	23602.0
64	18641.0
65	13680.0
66	10432.0
67	7184.0
68	5661.0
69	4138.0
70	3720.0
71	3302.0
72	2715.0
73	2128.0
74	1658.5
75	859.5
76	530.0
77	378.0
78	226.0
79	207.0
80	188.0
81	141.5
82	95.0
83	66.0
84	37.0
85	30.0
86	23.0
87	16.5
88	10.0
89	10.5
90	11.0
91	7.5
92	4.0
93	3.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1520465.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.045265006988885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.21573872373578	24.423535451055383
2	9.2340835149182	5.918173066644424
3	3.787640838352117	3.641278632488615
4	1.995622325521941	2.558009851008562
5	1.2302769206097206	1.9712274976460367
6	0.8677413959070026	1.668420179362462
7	0.6476949352800742	1.4528889091314148
8	0.5291294591791966	1.3564874993921652
9	0.44220041377040037	1.2753386500925357
>10	4.2077590046786115	29.025012707577012
>50	0.6492598847485872	14.671428218674198
>100	0.18625228295126056	7.82964537387665
>500	0.004537706488655127	0.9320909645680029
>1k	0.0018563344726316427	1.2031521648015429
>5k	0.0	0.0
>10k+	2.062593858479603E-4	2.0733108336809756
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31368	2.063053079156705	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTT	2777	0.18264149454278789	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2677	0.1760645591973508	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	2193	0.1442321921254353	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTC	2003	0.13173601496910484	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCT	2002	0.1316702456156505	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1806	0.1187794523385938	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1693	0.11134751539824987	No Hit
CGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTG	1583	0.10411288651826908	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.576935345437086E-5	0.0	0.0	0.07122820979108364	0.0
2	6.576935345437086E-5	0.0	0.0	0.2404527562291799	0.0
3	6.576935345437086E-5	0.0	0.0	0.4656470224569457	0.0
4	6.576935345437086E-5	0.0	0.0	0.635397723722677	0.0
5	6.576935345437086E-5	0.0	0.0	1.1939110732571943	0.0
6	6.576935345437086E-5	0.0	0.0	1.9667009763460521	0.0
7	6.576935345437086E-5	0.0	0.0	2.5087719875169765	0.0
8	6.576935345437086E-5	0.0	0.0	3.4170467587218383	0.0
9	6.576935345437086E-5	0.0	0.0	3.904463437172181	0.0
10	6.576935345437086E-5	0.0	0.0	4.451072533731457	0.0
11	6.576935345437086E-5	0.0	0.0	5.094954504049748	0.0
12	6.576935345437086E-5	0.0	0.0	5.539949949522021	0.0
13	6.576935345437086E-5	0.0	0.0	5.759422281999257	0.0
14	6.576935345437086E-5	0.0	0.0	5.899576774210521	0.0
15	6.576935345437086E-5	0.0	0.0	6.0303920182312645	0.0
16	6.576935345437086E-5	0.0	0.0	6.25302127967431	0.0
17	6.576935345437086E-5	0.0	0.0	6.548654523451707	0.0
18	6.576935345437086E-5	0.0	0.0	6.893088627492247	0.0
19	6.576935345437086E-5	0.0	0.0	7.109075184236401	0.0
20	6.576935345437086E-5	0.0	0.0	7.336900224602342	0.0
21	6.576935345437086E-5	0.0	0.0	7.607343806006715	0.0
22	6.576935345437086E-5	0.0	0.0	7.934743647502573	0.0
23	6.576935345437086E-5	0.0	0.0	8.25280424080791	0.0
24	6.576935345437086E-5	0.0	0.0	8.497203158244353	0.0
25	6.576935345437086E-5	0.0	0.0	8.706349702229252	0.0
26	6.576935345437086E-5	0.0	0.0	8.91003738987744	0.0
27	6.576935345437086E-5	0.0	0.0	9.136875889941564	0.0
28	6.576935345437086E-5	0.0	0.0	9.377262876817289	0.0
29	6.576935345437086E-5	0.0	0.0	9.632053352099522	0.0
30	6.576935345437086E-5	0.0	0.0	9.960110887129924	0.0
31	6.576935345437086E-5	0.0	0.0	10.224569457369949	0.0
32	6.576935345437086E-5	0.0	0.0	10.470546839289296	0.0
33	6.576935345437086E-5	0.0	0.0	10.727573472588977	0.0
34	6.576935345437086E-5	0.0	0.0	10.997753976579533	0.0
35	6.576935345437086E-5	0.0	0.0	11.303186854021632	0.0
36	6.576935345437086E-5	0.0	0.0	11.57823429016781	0.0
37	6.576935345437086E-5	0.0	0.0	11.860713663254334	0.0
38	6.576935345437086E-5	0.0	0.0	12.178445409792399	0.0
39	6.576935345437086E-5	0.0	0.0	12.651458599836234	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACGG	30	2.1659653E-6	45.000004	2
AGCGCGT	20	7.034006E-4	45.0	37
ATCGCCG	20	7.034006E-4	45.0	26
TAGTACG	35	1.2122837E-7	45.0	1
CGTTTTT	10875	0.0	44.068966	1
TACGGGA	665	0.0	42.293232	4
CGACGGT	635	0.0	41.811024	28
AACGCCG	205	0.0	41.707317	13
TCACGAC	645	0.0	41.51163	25
GACGGTC	645	0.0	41.51163	29
CGCATCG	190	0.0	41.44737	21
ACGGTCT	645	0.0	41.162792	30
CACGACG	640	0.0	41.132812	26
GCGCGAC	285	0.0	41.05263	9
GGCCGAT	220	0.0	40.90909	8
CGGTCTA	655	0.0	40.87786	31
CCAATCG	105	0.0	40.714287	24
CCGCTCG	100	0.0	40.5	19
CGCCGAC	100	0.0	40.5	13
TAACGCC	230	0.0	40.108692	12
>>END_MODULE