FastQCFastQC Report
Thu 26 May 2016
SRR1548117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548117_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1769500
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65540.3703871150042385No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA253.8916627E-545.038
TGCGATT207.034321E-445.033
GCGATAC253.8916627E-545.09
CGCGTAC207.034321E-445.017
TCGATCT302.166169E-644.99999621
ATCGGAT302.166169E-644.99999626
CCGTAAG800.042.18751
CGGTCTA6850.041.38686431
CGACGGT6800.041.360328
TTTACGG2300.041.086962
CGTAAGG2850.041.052632
CGTTTTT28700.041.0017431
CTATGCG556.184564E-1140.9090921
CTACGCG556.184564E-1140.9090921
TAGGGAC23450.040.778255
TTAGGGA32250.040.7441864
CTCACGA7300.039.45205724
CGAATAA403.459627E-739.37529
CGTCGAA403.459627E-739.37531
TACGTTC403.459627E-739.37531
Produced by FastQC (version 0.11.5)