##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548117_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1769500
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57122124894038	31.0	31.0	33.0	30.0	34.0
2	31.99043571630404	31.0	31.0	34.0	30.0	34.0
3	32.08141056795705	33.0	31.0	34.0	30.0	34.0
4	35.782735235942354	37.0	35.0	37.0	35.0	37.0
5	35.67416614862956	37.0	35.0	37.0	33.0	37.0
6	35.74094942074032	37.0	35.0	37.0	33.0	37.0
7	36.10807120655552	37.0	35.0	37.0	35.0	37.0
8	36.1474552133371	37.0	35.0	37.0	35.0	37.0
9	37.9961949703306	39.0	39.0	39.0	35.0	39.0
10	37.39750551003108	39.0	37.0	39.0	35.0	39.0
11	37.059441650183665	39.0	37.0	39.0	33.0	39.0
12	36.42495620231704	38.0	35.0	39.0	33.0	39.0
13	36.183430347555806	38.0	35.0	39.0	32.0	39.0
14	37.17096920033908	39.0	35.0	41.0	32.0	41.0
15	37.420962418762365	39.0	35.0	41.0	32.0	41.0
16	37.55293359706132	39.0	35.0	41.0	33.0	41.0
17	37.50269511161345	39.0	35.0	41.0	33.0	41.0
18	37.42862277479514	39.0	35.0	41.0	33.0	41.0
19	37.39589714608646	39.0	35.0	41.0	32.0	41.0
20	37.29698615428087	39.0	35.0	41.0	32.0	41.0
21	37.16844080248658	39.0	35.0	41.0	32.0	41.0
22	37.08443402090986	39.0	35.0	41.0	32.0	41.0
23	37.0280486012998	39.0	35.0	41.0	32.0	41.0
24	37.04733201469342	39.0	35.0	41.0	32.0	41.0
25	37.03459564848827	39.0	35.0	41.0	32.0	41.0
26	36.926057078270695	39.0	35.0	41.0	32.0	41.0
27	36.871170952246395	39.0	35.0	41.0	32.0	41.0
28	36.85268437411698	38.0	35.0	41.0	32.0	41.0
29	36.81605933879627	38.0	35.0	41.0	31.0	41.0
30	36.75278722803052	38.0	35.0	41.0	31.0	41.0
31	36.66763266459452	38.0	35.0	41.0	31.0	41.0
32	36.57261486295564	38.0	35.0	41.0	31.0	41.0
33	36.50452387680136	38.0	35.0	41.0	31.0	41.0
34	36.4313574456061	38.0	35.0	41.0	30.0	41.0
35	36.302305170952245	38.0	35.0	41.0	30.0	41.0
36	36.139801073749645	38.0	35.0	41.0	30.0	41.0
37	36.04509013845719	38.0	35.0	41.0	30.0	41.0
38	35.904701893190165	38.0	35.0	40.0	29.0	41.0
39	35.80967674484317	38.0	35.0	40.0	29.0	41.0
40	35.62060864651031	38.0	35.0	40.0	28.0	41.0
41	35.53336366205143	38.0	35.0	40.0	28.0	41.0
42	35.419147216727886	38.0	34.0	40.0	27.0	41.0
43	35.2924877083922	38.0	34.0	40.0	27.0	41.0
44	35.081874540830746	38.0	34.0	40.0	26.0	41.0
45	34.96127889234247	38.0	34.0	40.0	25.0	41.0
46	34.81972421588019	37.0	34.0	40.0	24.0	41.0
47	34.72283017801639	37.0	34.0	40.0	24.0	41.0
48	34.616040124328904	37.0	34.0	40.0	24.0	41.0
49	34.50533031929924	37.0	34.0	40.0	24.0	41.0
50	34.33405142695677	37.0	34.0	40.0	23.0	41.0
51	33.937653574456064	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	2.0
13	8.0
14	4.0
15	38.0
16	95.0
17	208.0
18	494.0
19	976.0
20	1752.0
21	2931.0
22	4647.0
23	7029.0
24	10614.0
25	15056.0
26	20466.0
27	25203.0
28	29516.0
29	34710.0
30	42048.0
31	52297.0
32	65105.0
33	83519.0
34	139588.0
35	218476.0
36	117704.0
37	153071.0
38	248543.0
39	495300.0
40	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.074088725628705	24.55478948855609	31.75682396157107	12.614297824244137
2	28.989884148064426	27.019610059338795	30.414184797965525	13.576320994631253
3	28.624639728736934	26.148516530093247	31.5884148064425	13.638428934727322
4	25.980333427521902	27.808137892059904	31.410172365074878	14.801356315343316
5	23.63605538287652	32.07996609211642	30.222661768861258	14.061316756145803
6	21.710087595365923	41.183950268437414	26.658038994066118	10.447923142130545
7	91.44707544504097	2.9055665442215313	4.269850240180842	1.3775077705566545
8	92.46979372704153	2.033342752189884	3.934049166431195	1.5628143543373836
9	87.8519920881605	5.0288782141847985	5.130884430630121	1.9882452670245834
10	42.833794857304326	38.320486012998025	10.713930488838654	8.131788640859
11	35.78768013563153	20.520599039276632	27.068719977394746	16.62300084769709
12	34.338287651879064	21.441989262503533	27.350777055665443	16.868946029951964
13	24.1988697372139	31.52308561740605	26.29262503532071	17.98541961005934
14	16.77620796835264	35.04792314213054	28.709635490251483	19.466233399265327
15	15.130036733540548	29.238654987284544	38.66137326928511	16.9699350098898
16	20.284882735235943	26.217066968070075	36.5792031647358	16.91884713195818
17	21.003899406612035	25.834981633229724	26.60406894602995	26.557050014128286
18	21.67295846284261	28.186549872845433	31.30347555806725	18.837016106244704
19	25.34636903079966	27.94083074314778	26.56106244701893	20.151737779033628
20	26.88522181407177	28.031195252896296	26.667250635772817	18.416332297259114
21	24.030008476970895	28.872845436564	29.887369313365358	17.209776773099748
22	23.64543656400113	23.377846849392483	29.506075162475277	23.47064142413111
23	20.192540265611754	28.304436281435436	29.394970330601865	22.108053122350945
24	19.53580107374965	26.810680983328623	35.32647640576434	18.327041537157392
25	19.260299519638316	28.702006216445326	31.947329754167843	20.090364509748518
26	18.61339361401526	34.70912687199774	26.107318451539985	20.570161062447017
27	17.951737779033625	33.8172930206273	30.243854196100596	17.987115004238486
28	16.129076010172366	29.263577281718	36.04950551003108	18.557841198078552
29	17.25617406046906	26.55851935575021	34.793048883865495	21.39225769991523
30	19.724950551003108	29.883187341056793	31.63978525007064	18.752076857869454
31	25.2959593105397	27.570217575586327	27.49872845436564	19.635094659508336
32	25.942413111048317	27.452557219553547	27.657360836394464	18.94766883300367
33	24.077140435151172	27.617010454930774	27.859508335688048	20.44634077423001
34	19.50901384571913	26.923481209381183	31.092059903927666	22.475445040972026
35	20.663972873693133	26.289516812658942	30.992314213054534	22.054196100593387
36	25.16631816897429	26.127606668550442	29.719355750211925	18.98671941226335
37	20.014975981915796	29.809607233681827	30.38705849109918	19.788358293303194
38	20.305283978525008	30.464142413111052	27.988132240745973	21.24244136761797
39	19.842271828200055	29.458038994066122	29.337892059903925	21.361797117829894
40	22.97389092964114	26.67131958180277	28.82599604408025	21.52879344447584
41	19.462277479513986	24.651879061881885	29.699067533201468	26.186775925402657
42	20.786945464820572	25.69816332297259	28.011302627860978	25.50358858434586
43	20.893246679853068	26.817462560045215	28.089177733823114	24.20011302627861
44	20.585871715173777	28.52415936705284	29.066459451822546	21.82350946595083
45	18.701102006216445	32.615823679005366	26.394857304323256	22.28821701045493
46	21.41825374399548	29.99090138457191	28.252331166996324	20.33851370443628
47	20.877649053404916	26.663803334275222	29.851483469906753	22.607064142413112
48	21.05611754732975	26.34738626730715	31.8970895733258	20.6994066120373
49	20.827182820005653	25.690703588584345	31.36196665724781	22.120146934162193
50	19.940661203729867	28.85493077140435	30.038937552981064	21.165470471884714
51	18.705283978525006	30.329584628426108	27.915117264764056	23.050014128284825
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	770.0
1	1756.0
2	2742.0
3	6480.5
4	10219.0
5	7319.0
6	4419.0
7	4226.0
8	4033.0
9	4267.5
10	4502.0
11	4579.0
12	4656.0
13	4472.5
14	4289.0
15	4210.0
16	4131.0
17	3913.5
18	3696.0
19	3792.5
20	3889.0
21	5367.0
22	6845.0
23	8300.5
24	9756.0
25	11934.5
26	17812.0
27	21511.0
28	28398.5
29	35286.0
30	39048.5
31	42811.0
32	50531.0
33	58251.0
34	61398.0
35	64545.0
36	70182.0
37	75819.0
38	82097.5
39	88376.0
40	110004.0
41	131632.0
42	146210.5
43	160789.0
44	159899.5
45	159010.0
46	156948.0
47	154886.0
48	149329.0
49	143772.0
50	137080.5
51	130389.0
52	118929.0
53	107469.0
54	96922.0
55	86375.0
56	77007.0
57	67639.0
58	59689.0
59	51739.0
60	44855.5
61	37972.0
62	32460.0
63	26948.0
64	22812.0
65	18676.0
66	14817.0
67	10958.0
68	8734.0
69	6510.0
70	5231.5
71	3953.0
72	3336.5
73	2720.0
74	2268.5
75	1243.0
76	669.0
77	576.5
78	484.0
79	360.0
80	236.0
81	152.5
82	69.0
83	59.5
84	50.0
85	43.5
86	37.0
87	26.0
88	15.0
89	15.0
90	15.0
91	12.5
92	10.0
93	5.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1769500.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.980536223869983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.2330457175025	18.897594859102227
2	7.18722798657986	3.3033270618962116
3	2.2187936715964045	1.5296720503024395
4	1.05042194636946	0.9655703835556554
5	0.6228137320170376	0.7156296764670593
6	0.4073187046528119	0.5616241348160246
7	0.310895920769385	0.5001188478365984
8	0.26613637578725957	0.4892765299414874
9	0.20233105226760625	0.4184708468264511
>10	3.016244802956151	17.690383599759528
>50	1.6004910799099152	26.709324677743453
>100	0.8810751001386431	27.250804954005485
>500	0.001971636586474463	0.3115012037210133
>1k	9.858182932372314E-4	0.2855050123559324
>5k	2.4645457330930786E-4	0.3711961616704584
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6554	0.3703871150042385	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05481774512574174	0.0
2	0.0	0.0	0.0	0.1947442780446454	0.0
3	0.0	0.0	0.0	0.28912122068380897	0.0
4	0.0	0.0	0.0	0.43684656682678724	0.0
5	0.0	0.0	0.0	0.7491381746256005	0.0
6	0.0	0.0	0.0	1.013111048318734	0.0
7	0.0	0.0	0.0	1.1870584910991806	0.0
8	0.0	0.0	0.0	1.635885843458604	0.0
9	0.0	0.0	0.0	1.771065272675897	0.0
10	0.0	0.0	0.0	2.0504097202599603	0.0
11	0.0	0.0	0.0	2.502232269002543	0.0
12	0.0	0.0	0.0	2.866289912404634	0.0
13	0.0	0.0	0.0	3.0073467081096354	0.0
14	0.0	0.0	0.0	3.058604125459169	0.0
15	0.0	0.0	0.0	3.1500988979937836	0.0
16	0.0	0.0	0.0	3.3551850805312236	0.0
17	0.0	0.0	0.0	3.6313082791749083	0.0
18	0.0	0.0	0.0	3.9467646227747952	0.0
19	0.0	0.0	0.0	4.143147781859282	0.0
20	5.6513139304888384E-5	0.0	0.0	4.370048036168409	0.0
21	5.6513139304888384E-5	0.0	0.0	4.64023735518508	0.0
22	1.1302627860977677E-4	0.0	0.0	4.95478948855609	0.0
23	1.1302627860977677E-4	0.0	0.0	5.2599604408024865	0.0
24	1.1302627860977677E-4	0.0	0.0	5.487821418479797	0.0
25	1.1302627860977677E-4	0.0	0.0	5.692568522181407	0.0
26	1.1302627860977677E-4	0.0	0.0	5.885786945464821	0.0
27	1.1302627860977677E-4	0.0	0.0	6.085730432325516	0.0
28	1.1302627860977677E-4	0.0	0.0	6.3081096354902515	0.0
29	1.1302627860977677E-4	0.0	0.0	6.551568239615711	0.0
30	1.1302627860977677E-4	0.0	0.0	6.875897146086465	0.0
31	1.1302627860977677E-4	0.0	0.0	7.130827917490817	0.0
32	1.1302627860977677E-4	0.0	0.0	7.354111330884431	0.0
33	1.1302627860977677E-4	0.0	0.0	7.580276914382594	0.0
34	1.1302627860977677E-4	0.0	0.0	7.842271828200056	0.0
35	1.1302627860977677E-4	0.0	0.0	8.14184797965527	0.0
36	1.1302627860977677E-4	0.0	0.0	8.397004803616841	0.0
37	1.1302627860977677E-4	0.0	0.0	8.656626165583498	0.0
38	1.6953941791466516E-4	0.0	0.0	8.943430347555807	0.0
39	1.6953941791466516E-4	0.0	0.0	9.356484882735236	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAA	25	3.8916627E-5	45.0	38
TGCGATT	20	7.034321E-4	45.0	33
GCGATAC	25	3.8916627E-5	45.0	9
CGCGTAC	20	7.034321E-4	45.0	17
TCGATCT	30	2.166169E-6	44.999996	21
ATCGGAT	30	2.166169E-6	44.999996	26
CCGTAAG	80	0.0	42.1875	1
CGGTCTA	685	0.0	41.386864	31
CGACGGT	680	0.0	41.3603	28
TTTACGG	230	0.0	41.08696	2
CGTAAGG	285	0.0	41.05263	2
CGTTTTT	2870	0.0	41.001743	1
CTATGCG	55	6.184564E-11	40.909092	1
CTACGCG	55	6.184564E-11	40.909092	1
TAGGGAC	2345	0.0	40.77825	5
TTAGGGA	3225	0.0	40.744186	4
CTCACGA	730	0.0	39.452057	24
CGAATAA	40	3.459627E-7	39.375	29
CGTCGAA	40	3.459627E-7	39.375	31
TACGTTC	40	3.459627E-7	39.375	31
>>END_MODULE