FastQCFastQC Report
Thu 26 May 2016
SRR1548116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548116_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2013901
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65970.32757320245632726No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAG351.2125383E-745.030
CGACGGT5800.042.28448528
CGGTCTA5800.042.28448531
CACGACG5900.041.94915426
CGCATCG700.041.78571321
TACGGGA10850.041.6820264
ATATGCG603.6379788E-1241.2500041
TCTAGCG603.6379788E-1241.2500041
TACGTAG1150.041.086961
TCACGAC6200.041.00806825
CGTAAGG2200.040.9090922
CGTTAGG4100.040.6097562
CGTTTTT33950.040.294551
TAGGGAT34500.039.7826085
ACGGTCT6150.039.51219630
TTAGGGA28800.039.3754
TAGGGAC22150.039.209935
TACGGGT750.039.0000044
GTAGGGT9800.038.571434
CCCCGTA356.2506333E-638.57142623
Produced by FastQC (version 0.11.5)