FastQCFastQC Report
Thu 26 May 2016
SRR1548115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548115_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1188095
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50470.42479768032017645No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23960.2016673750836423No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA253.8908845E-545.00000423
CGCGGAC253.8908845E-545.00000424
CTATCCG253.8908845E-545.00000445
CTATACG302.1655633E-645.0000041
CGACCTT253.8908845E-545.00000429
TCCTCGA302.1655633E-645.00000423
TAACGTG253.8908845E-545.00000420
CGTTACG253.8908845E-545.00000444
TACGAGA253.8908845E-545.00000434
TCGCTCC302.1655633E-645.00000423
TCTCGAT302.1655633E-645.00000414
CCGCGGA253.8908845E-545.00000423
CGCGTTC253.8908845E-545.00000432
ACGACAG600.045.0000041
ACGCGTT302.1655633E-645.00000410
CGGAATT207.0333836E-445.029
GTACCGT207.0333836E-445.010
CGAACAA351.2119744E-745.039
GTCGAGC207.0333836E-445.025
ACGTTAT207.0333836E-445.020