FastQCFastQC Report
Thu 26 May 2016
SRR1548114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548114_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1671244
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162520.9724492653376765No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCG351.2123746E-745.0000041
ACTCGCG207.034207E-445.01
TACTCGC253.8915696E-545.045
TACTCCG207.034207E-445.043
TATCACG302.1660962E-644.9999961
CGGTCTA3200.044.29687531
CGTTTTT69450.043.0561561
CGACGGT3350.042.98507328
GATCGAC556.184564E-1140.9090929
CGCCGGT556.184564E-1140.90909228
CTCGCGC2000.040.533
TGCGCGG1950.040.3846172
TCGCGAG850.039.7058831
ATGCGAG1250.039.6000021
GACGGTC3650.039.45205329
ACGGTCT3650.039.45205330
TAGTTCG403.4594814E-739.3751
TCGCGCG403.4594814E-739.3751
TATAGCG1200.039.3749961
CGGAATC356.249991E-638.5714345
Produced by FastQC (version 0.11.5)