##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548114_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1671244
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.492495410604317	31.0	31.0	33.0	30.0	34.0
2	31.902445124709498	31.0	31.0	34.0	30.0	34.0
3	31.888269456763943	33.0	31.0	34.0	30.0	34.0
4	35.6606043163057	37.0	35.0	37.0	33.0	37.0
5	35.59547498749435	37.0	35.0	37.0	33.0	37.0
6	35.66647120348674	37.0	35.0	37.0	33.0	37.0
7	36.06378123122656	37.0	35.0	37.0	35.0	37.0
8	36.10500142408888	37.0	35.0	37.0	35.0	37.0
9	37.937237171831285	39.0	38.0	39.0	35.0	39.0
10	37.314746978897155	39.0	37.0	39.0	34.0	39.0
11	36.9907589795386	39.0	37.0	39.0	33.0	39.0
12	36.59548994641118	39.0	35.0	39.0	32.0	39.0
13	36.45592385073634	39.0	35.0	39.0	32.0	39.0
14	37.53978413684656	40.0	36.0	41.0	32.0	41.0
15	37.69766293850569	40.0	36.0	41.0	33.0	41.0
16	37.788903355823564	40.0	36.0	41.0	33.0	41.0
17	37.71872509340348	40.0	36.0	41.0	33.0	41.0
18	37.59768053019188	39.0	36.0	41.0	33.0	41.0
19	37.52291347044477	39.0	36.0	41.0	32.0	41.0
20	37.372691240776334	39.0	35.0	41.0	32.0	41.0
21	37.26522817733377	39.0	35.0	41.0	32.0	41.0
22	37.178572967202875	39.0	35.0	41.0	32.0	41.0
23	37.08430725854513	39.0	35.0	41.0	32.0	41.0
24	37.09311626548846	39.0	35.0	41.0	32.0	41.0
25	37.07735555071552	39.0	35.0	41.0	32.0	41.0
26	36.95932012321361	39.0	35.0	41.0	32.0	41.0
27	36.90917544057002	39.0	35.0	41.0	31.0	41.0
28	36.862547898451695	39.0	35.0	41.0	31.0	41.0
29	36.820464276909895	39.0	35.0	41.0	31.0	41.0
30	36.71999360955073	38.0	35.0	41.0	31.0	41.0
31	36.62112175122244	39.0	35.0	41.0	31.0	41.0
32	36.4701796984761	39.0	35.0	41.0	30.0	41.0
33	36.33708722364897	39.0	35.0	41.0	30.0	41.0
34	36.170936140982406	39.0	35.0	41.0	30.0	41.0
35	36.01610476986006	38.0	35.0	41.0	29.0	41.0
36	35.8450334002695	38.0	35.0	41.0	29.0	41.0
37	35.772949371845165	38.0	35.0	41.0	28.0	41.0
38	35.67347736177363	38.0	35.0	41.0	27.0	41.0
39	35.62204501556924	38.0	35.0	40.0	27.0	41.0
40	35.5322568098973	38.0	35.0	40.0	27.0	41.0
41	35.462658953450244	38.0	35.0	40.0	27.0	41.0
42	35.411091976994385	38.0	35.0	40.0	26.0	41.0
43	35.32901359705704	38.0	34.0	40.0	26.0	41.0
44	35.17242963923879	38.0	34.0	40.0	26.0	41.0
45	35.162204322049924	38.0	34.0	40.0	26.0	41.0
46	35.085681085466874	38.0	34.0	40.0	25.0	41.0
47	35.02490180966992	38.0	34.0	40.0	25.0	41.0
48	34.906694653802795	38.0	34.0	40.0	24.0	41.0
49	34.79461048177286	38.0	34.0	40.0	24.0	41.0
50	34.66452115908868	37.0	34.0	40.0	24.0	41.0
51	34.305058986000844	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	4.0
14	12.0
15	26.0
16	86.0
17	214.0
18	525.0
19	1103.0
20	1930.0
21	3177.0
22	5191.0
23	7820.0
24	11837.0
25	17998.0
26	24026.0
27	27724.0
28	29607.0
29	32658.0
30	37427.0
31	44989.0
32	55987.0
33	72204.0
34	114833.0
35	171194.0
36	118636.0
37	154983.0
38	248291.0
39	488692.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.594960400755365	24.709497835145555	30.94066455885556	14.754877205243519
2	31.35568474740971	25.883653135029956	29.544159919197917	13.216502198362418
3	27.61098917931792	24.790934178372517	34.08233627166351	13.515740370646057
4	25.064323342372507	27.08240089418421	33.68329220628466	14.16998355715862
5	23.390540220338863	31.82629227090718	31.13417310697899	13.648994401774967
6	22.025030456354667	40.305784194288805	27.90119216583575	9.76799318352078
7	89.01465016478743	2.577900055288157	7.186622659527872	1.2208271203965428
8	90.05220063617281	1.9466337650277281	6.771841813643011	1.2293237851564462
9	84.97185330209113	5.071671162319805	7.916797307873656	2.0396782277154024
10	38.762801841023816	37.77240187548916	14.675535110372874	8.78926117311416
11	28.708195811024602	22.915923707130737	31.363224041492444	17.012656440352217
12	29.30625330592062	22.37584697387096	29.99855197685078	18.319347743357643
13	23.657945817606528	28.226399017737684	30.448994880460305	17.666660284195483
14	17.34635995701406	31.469073336987297	31.507248492739542	19.677318213259102
15	16.48023867250982	29.720136616795635	37.51139869462508	16.28822601606947
16	21.004832328493027	28.082254895155945	35.39668654008631	15.516226236264721
17	21.23825126672108	27.441474733791114	29.641213371596248	21.67906062789156
18	21.452762134074977	28.339488428978655	32.66076048739741	17.546988949548957
19	24.029285969014698	29.24534059658554	28.77413471641484	17.95123871798493
20	25.723772231942192	29.51089128816618	28.805548441759555	15.959788038132075
21	24.185816074732355	27.47988923221265	32.093398689838224	16.240896003216765
22	23.429612911100953	23.69642015169538	32.10267321827334	20.77129371893033
23	20.16473955927441	27.380203010452092	32.541747345091444	19.913310085182058
24	18.790194609524402	27.349567148782583	35.7104647795295	18.149773462163516
25	18.956358257681106	29.78697305719572	33.03604979284892	18.220618892274256
26	18.86600640002298	34.04900780496444	28.723753084528653	18.361232710483925
27	17.98414833501272	32.93845781944468	31.86494611199801	17.212447733544593
28	16.862648422372793	29.482648853189602	35.50594646861859	18.148756255819016
29	17.470638638044473	27.72707037392505	35.22920650724849	19.57308448078198
30	18.93978377783256	29.45919327159888	32.75637788377998	18.84464506678857
31	22.683402303912533	28.227296552747532	29.81389910749119	19.275402035848746
32	23.045527762552926	28.736677588670474	29.96049649243318	18.257298156343417
33	22.75305102067681	28.467596592717758	29.13434543370088	19.64500695290454
34	19.355761337063885	28.657515000801798	31.002414967533166	20.984308694601147
35	19.418528952085993	28.414283013132728	31.16786058768199	20.999327447099287
36	22.28435823853369	28.98182431769388	30.065448252918188	18.66836919085424
37	19.679771475619358	30.608397098209476	30.422607351170743	19.28922407500042
38	19.23261953371261	31.00510757256271	29.50706180545749	20.255211088267185
39	19.086321327107232	30.78425412447255	30.187752356926932	19.941672191493282
40	21.459882578486443	28.77598962210186	29.54074928616049	20.223378513251205
41	18.975565506891872	27.61152770032383	30.442053943050805	22.970852849733493
42	20.12111935779575	27.911424064948026	29.252520876664327	22.714935700591894
43	19.936705831105453	28.595525249454894	29.803667208378908	21.66410171106074
44	19.63028737874302	30.11337662244412	29.741856964033975	20.514479034778883
45	18.72892288618538	32.69971350682486	27.725095796903382	20.846267810086378
46	20.08384173705336	31.36471993317553	28.043720725399762	20.507717604371354
47	19.724648226111807	29.205490042148245	29.704998192962844	21.3648635387771
48	20.120580836789838	28.49093250297383	30.92468843568025	20.46379822455608
49	19.940654985148786	28.250632463003605	30.981831497973968	20.82688105387364
50	18.666454449499895	30.552091735258287	30.204027658438864	20.577426156802954
51	18.35141966104291	31.510898468446257	29.008211846983446	21.129470023527382
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	866.0
1	1627.5
2	2389.0
3	13440.5
4	24492.0
5	17343.5
6	10195.0
7	9540.0
8	8885.0
9	9255.5
10	9626.0
11	10038.5
12	10451.0
13	10261.5
14	10072.0
15	9457.5
16	8843.0
17	8593.0
18	8343.0
19	8382.5
20	8422.0
21	8830.0
22	9238.0
23	10047.5
24	10857.0
25	13971.0
26	19663.5
27	22242.0
28	28915.5
29	35589.0
30	42024.5
31	48460.0
32	53886.5
33	59313.0
34	63408.0
35	67503.0
36	73466.0
37	79429.0
38	86446.5
39	93464.0
40	104863.0
41	116262.0
42	125485.5
43	134709.0
44	135829.0
45	136949.0
46	136857.0
47	136765.0
48	133994.0
49	131223.0
50	121368.0
51	111513.0
52	100768.0
53	90023.0
54	81919.0
55	73815.0
56	66201.0
57	58587.0
58	52396.0
59	46205.0
60	40184.5
61	34164.0
62	28255.5
63	22347.0
64	18403.0
65	14459.0
66	11369.5
67	8280.0
68	6632.0
69	4984.0
70	3737.5
71	2491.0
72	1945.5
73	1400.0
74	1043.5
75	520.0
76	353.0
77	235.5
78	118.0
79	83.5
80	49.0
81	46.5
82	44.0
83	34.5
84	25.0
85	20.5
86	16.0
87	10.5
88	5.0
89	5.5
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1671244.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.10900826598626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.83205843848118	19.005627485180547
2	7.228487781232806	3.485433433366448
3	2.6404882897963096	1.9097866201481746
4	1.4045973900939561	1.3545380035263166
5	0.9202770975003703	1.1093484075317137
6	0.6663251076043555	0.9638662516240553
7	0.5097232212746969	0.8602244948553769
8	0.4511457003322738	0.8701340334779959
9	0.3596618559212526	0.7803981591628893
>10	4.962633522925286	29.060119777685156
>50	1.6419687393441635	27.788343905744604
>100	0.38089248514283114	11.54209250979651
>500	0.0012431216789901276	0.20577697810898837
>1k	2.4862433579802553E-4	0.09015105594987431
>5k	0.0	0.0
>10k+	2.4862433579802553E-4	0.9741588838413281
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16252	0.9724492653376765	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019027742208797757	0.0
2	0.0	0.0	0.0	0.07054625177412753	0.0
3	0.0	0.0	0.0	0.1304417547647142	0.0
4	0.0	0.0	0.0	0.21104039864914997	0.0
5	0.0	0.0	0.0	0.4190291782648135	0.0
6	0.0	0.0	0.0	0.6741684637312086	0.0
7	0.0	0.0	0.0	0.8573852770750411	0.0
8	0.0	0.0	0.0	1.1553669003448928	0.0
9	0.0	0.0	0.0	1.2809619660564227	0.0
10	0.0	0.0	0.0	1.470521360136521	0.0
11	0.0	0.0	0.0	1.760604675319702	0.0
12	0.0	0.0	0.0	1.9730811299846103	0.0
13	0.0	0.0	0.0	2.067980498359306	0.0
14	0.0	0.0	0.0	2.1157293608832703	0.0
15	0.0	0.0	0.0	2.1799330319211316	0.0
16	0.0	0.0	0.0	2.307383003319683	0.0
17	0.0	0.0	0.0	2.4795302182087116	0.0
18	0.0	0.0	0.0	2.66322571689113	0.0
19	0.0	0.0	0.0	2.7978559683684727	0.0
20	0.0	0.0	0.0	2.93440096120016	0.0
21	0.0	0.0	0.0	3.1124719071541915	0.0
22	0.0	0.0	0.0	3.3291966941990516	0.0
23	0.0	0.0	0.0	3.547596879928963	0.0
24	0.0	0.0	0.0	3.7032892863040945	0.0
25	0.0	0.0	0.0	3.8444416255196727	0.0
26	0.0	0.0	0.0	3.978772698660399	0.0
27	0.0	0.0	0.0	4.123694684917343	0.0
28	0.0	0.0	0.0	4.28004528363303	0.0
29	0.0	0.0	0.0	4.455603131559485	0.0
30	0.0	0.0	0.0	4.669096792568888	0.0
31	0.0	0.0	0.0	4.865537288391162	0.0
32	0.0	0.0	0.0	5.052523748776361	0.0
33	0.0	0.0	0.0	5.22963732405322	0.0
34	0.0	0.0	0.0	5.424342585523119	0.0
35	0.0	0.0	0.0	5.639750987886868	0.0
36	0.0	0.0	0.0	5.846662725490712	0.0
37	0.0	0.0	0.0	6.062489977525724	0.0
38	0.0	0.0	0.0	6.288848307009629	0.0
39	0.0	0.0	0.0	6.5990962420807495	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	35	1.2123746E-7	45.000004	1
ACTCGCG	20	7.034207E-4	45.0	1
TACTCGC	25	3.8915696E-5	45.0	45
TACTCCG	20	7.034207E-4	45.0	43
TATCACG	30	2.1660962E-6	44.999996	1
CGGTCTA	320	0.0	44.296875	31
CGTTTTT	6945	0.0	43.056156	1
CGACGGT	335	0.0	42.985073	28
GATCGAC	55	6.184564E-11	40.909092	9
CGCCGGT	55	6.184564E-11	40.909092	28
CTCGCGC	200	0.0	40.5	33
TGCGCGG	195	0.0	40.384617	2
TCGCGAG	85	0.0	39.705883	1
ATGCGAG	125	0.0	39.600002	1
GACGGTC	365	0.0	39.452053	29
ACGGTCT	365	0.0	39.452053	30
TAGTTCG	40	3.4594814E-7	39.375	1
TCGCGCG	40	3.4594814E-7	39.375	1
TATAGCG	120	0.0	39.374996	1
CGGAATC	35	6.249991E-6	38.57143	45
>>END_MODULE