FastQCFastQC Report
Thu 26 May 2016
SRR1548113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548113_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2140526
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69070.32267769697728504No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24470.11431769574394332No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGG207.03465E-445.0000042
CGTATAA207.03465E-445.00000439
CGTCGAT207.03465E-445.00000411
ACCGTTC406.8193913E-945.00000415
ACCCGTA406.8193913E-945.00000419
CGCAATA207.03465E-445.00000427
CTATCGT351.2125929E-745.019
CGAACGC302.1663836E-644.99999620
GCGAACG302.1663836E-644.99999611
GCGTAAG2400.044.0624961
CGGTCTA4650.042.58064731
TATAGCG2650.041.6037751
CGTTTTT33300.041.486491
CGTAAGG4400.041.4204562
ACCGGTA603.6379788E-1241.24999621
AGTACGG2950.039.661022
ACACGAT403.4600816E-739.37500444
GCCGAAC403.4600816E-739.37500418
TACGCGG1950.039.230772
ACGGGAT10350.039.1304365
Produced by FastQC (version 0.11.5)