FastQCFastQC Report
Thu 26 May 2016
SRR1548112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548112_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2197211
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167360.7616928915793704No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG27300.12424842220433086No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG551.8189894E-1245.01
CGTATTA207.0346927E-445.041
GCGCCTA253.891971E-545.020
GAGCGTA351.2126111E-745.09
ACTACGC207.0346927E-445.021
CGTCGAG253.891971E-545.037
TCGACTA207.0346927E-445.030
TCGACCG253.891971E-545.013
TACGCGG1250.044.9999962
TACGTAG1750.043.7142871
CGGTCTA5700.043.42105531
TCTAGCG1050.042.8571431
CGACCCG1000.042.7532
ACGTAGG3850.042.6623342
CGTTTTT59850.042.51881
CGGGTAT1500.042.06
CTATGCG700.041.7857131
CGTTAGG3300.041.590912
TAGTACG650.041.538461
TCACGAC6050.041.28099425
Produced by FastQC (version 0.11.5)